Identification of plasma miRNA biomarkers for pregnancy detection in dairy cattle

Lim, Hyun-Joo; Kim, Hyun-Jong; Lee, Jihwan; Lim, Dong-Hyun; Son, Jun-Kyu; 김은태,; Jang, Gul Won; Kim, Dong‐Hyeon

doi:10.12750/jarb.36.1.35

Cited by 11 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these, 22 were differentially expressed in microarray results upon pregnancy groups were compared with cyclic ( Table 1 ). The results may indicate that circulating plasma miRNAs may function as potential biomarkers in the regulation of pregnancy during the estrous cycle and early pregnancy ( Ioannidis and Donadeu, 2017 ; Lim et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

View full text Add to dashboard Cite

MicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

View full text Add to dashboard Cite

show abstract

“…There were 208 miRNAs found in cattle, and comparing plasma samples taken from pregnant and non-pregnant animals, sixteen showed differential expression ( Ioannidis and Donadeu, 2016 ). According to these findings, it may be inferred that plasma miRNAs are circulating and may function as biomarkers for early pregnancy and the estrous cycle ( Lim et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Expression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma

Ucar,

Hitit,

Kose

et al. 2024

Anim. Reprod.

View full text Add to dashboard Cite

In the current study, we aimed to assess the expression levels of two circulating microRNAs (miRNA) (oar-miR-485-5p and oar-miR-493-5p) in the ovine plasma during the peri-implantation. After mating, we collected the plasma samples from a total of 8 ewes on day 22 of pregnancy (P22; n = 4) and day 22 of the estrous cycle (C22; n=4). We used mature miRNA sequences for oar-miR-485-5p and oar-miR-493-5p out of one hundred fifty, which were retrieved from our microarray results of previous study. We showed that the miRNA expression of oar-miR-485-5p and oar-miR-493-5p were upregulated in P22 (P<0.05) when compared to C22. Those two miRNAs targeted 311 target genes in the peri-implantation period of pregnancy. Furthermore, we revealed 151 GO/pathway terms in biological process (BP) and 25 GO/pathway terms in molecular function (MF), while we demonstrated 13 GO/pathway terms in cellular component (CC). We revealed three hub genes as interleukin 2 (IL2), interleukin 18 (IL18), and C-X-C Motif Chemokine Ligand 10 (CXCL10). In conclusion, both miR-485-5p and oar-miR-493-5p have the potential to be a biomarker to understand peri-implantation of the ovine pregnancy in the aspect of pregnancy-reflected changes in maternal plasma.

show abstract

“…[ 29 ] For instance, the increase of bta‐mir‐140 in Exos correlates with early pregnancy in cows. [ 30 ] Regarding the fact that the metabolic status of parent cells can affect the exosomal content, thus FF Exos can be used as a prognostic and diagnostic tool for real‐time health monitoring of source cells in terms of fertility in females. [ 15 ] However, small volumes of FF samples and a lack of standard isolation protocol are the main challenges associated with the application of Exos for monitoring the physiology of the female reproductive system.…”

Section: Exos and The Female Reproductive Systemmentioning

confidence: 99%

Exosomes as Theranostic Agents in Reproduction System

Izadpanah,

Yalameha,

Sani

et al. 2023

Advanced Biology

View full text Add to dashboard Cite

Exosomes (Exos), belonging to extracellular vesicles, are cell‐derived nano‐sized vesicles with the potential to carry different kinds of biological molecules. Many studies have proved the impacts of exosomal cargo on several biological processes in female and male reproductive systems. It is also hypothesized that changes in exosomal cargo are integral to the promotion of certain pathological conditions, thus Exos can be used as valid biomarkers for the diagnosis of infertility and other abnormal conditions. Here, efforts are made to collect some recent data related to the physiological significance of Exos in the reproductive system, and their potential therapeutic effects. It is anticipated that the current review article will lay the groundwork for elucidating the source and mechanisms by which Exos control the reproductive system additionally supplying fresh methods and concepts for the detection and treatment of disorders associated with fertility for future studies.

show abstract

Identification of plasma miRNA biomarkers for pregnancy detection in dairy cattle

Cited by 11 publications

References 34 publications

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

Expression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma

Exosomes as Theranostic Agents in Reproduction System

Contact Info

Product

Resources

About