Identification of Petroleum Degrading Bacteria and Construction of Petroleum Degrading Agent

Abstract

The aim of this study is to construct petroleum degrading agent (PDA) which can effectively degrade oil. By enrichment, domestication, and separation of culture from the soil sample of Qianjiang Guanghua Oilfield, the pure culture of three petroleum degrading bacteria G-40, G-53, and G-94 was identified from the medium supplemented with oil, which served as the sole source of carbon. The species of G-40 and G-53 were preliminarily identified and classified by morphological observation, physiological and biochemical determination, and sequence analyses of 16S rDNA. The species of G-94 was preliminarily identified and classified by morphological observation, physiological and biochemical determination, and sequence analyses of ITS rDNA. The optimal inoculation proportion of these three bacteria strains and bran proportion in composition of PDA were determined through orthogonal test. G-40, G-53, and G-94 were isolated and identified as Brevibacillus laterosporus, Tsukamurella inchonensis, and Candida tropicalis, respectively.