Identification of Peroxisomes in the Epithelial Cells of the Small Intestine of the Guinea Pig

Connock, Martin; Kirk, Paul R.

doi:10.1177/21.5.502

Cited by 11 publications

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“…Preliminary experiments and previous work (8,9) showed that intestinal catalase particles are denser than other subcellular particles of their approximate size, but smaller than those organelles approaching their densi W. It can be expected, therefore, that separation on the basis of size differences combined with a second separation based GI G' IT on density should give considerable resolution between catalase particles and other organelles.…”

Section: R E S U L T Smentioning

confidence: 84%

“…Subsequently, fractionation data have extended this list by including subcellular particles in the goldfish intestine (7). In addition, peroxisome-like catalase particles have been identified in fractions isolated from homogenates of epithelial cells from the guinea pig small intestine (8,9). Like hepatic peroxisomes, the intestinal particles showed latency of catalase activity and had a relatively high density in sucrose density gradients.…”

Section: Introductionmentioning

confidence: 99%

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A Zonal Rotor Method for the Preparation of Microperoxisomes From Epithelial Cells of Guinea Pig Small Intestine

Connock

Kirk

Sturdee

1974

The Journal of Cell Biology

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A method is described for the preparation of catalase particles from homogenates made from suspensions of epithelial cells of the small intestine of the guinea pig. Electron microscope examination of the preparations revealed the presence of small diaminobenzidine-positive particles measuring 0.1–0.3 nm in diameter and resembling the microperoxisomes observed by Novikoff and Novikoff (1972. J. Cell Biol. 53:532.). Analytical data upon which the method is based are presented. The technique consisted of a rate zonal separation of microperoxisomes from large particles followed by an isopycnic separation from less dense organelles. Application of the method yielded microperoxisomes purified between 20- and 30-fold.

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Section: R E S U L T Smentioning

confidence: 84%