A Zonal Rotor Method for the Preparation of Microperoxisomes From Epithelial Cells of Guinea Pig Small Intestine

Connock, Martin; Kirk, Paul R.; Sturdee, A. P.

doi:10.1083/jcb.61.1.123

Cited by 36 publications

(2 citation statements)

References 27 publications

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“…Many microperoxisomes in the pigment epithelial cells appear circular or only slightly elongate (Figs. [1][2][3][4][5][6][7][8][9][10]. However, more elongate forms are encountered (Figs.…”

Section: Resultsmentioning

confidence: 99%

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Studies on microperoxisomes. VII. Pigment epithelial cells and other cell types in the retina of rodents.

Leuenberger¹,

Novikoff²

1975

The Journal of Cell Biology

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The pigment epithelial cell of the retina actively participates in two aspects of lipid metabolism: (a) the fatty acid esterification of vitamin A and its storage and transport to the photoreceptors, and (b) the phagocytosis and degradation of the lipoprotein membrane disks shed from the photoreceptor cells. Study of the pigment epithelial cells of adult albino and pigmented rodents has revealed the abundance of an organeUe, microperoxisomes, not previously known to exist in this cell type. The metabolism, transport, and storage of lipids are major functions of other cell types which possess large numbers of microperoxisomes associated with a highly developed smooth endoplasmic reticulum. Microperoxisomes were encountered, but relatively rarely, in MUller cells and vascular endothelial cells. A tubular system in photoreceptor terminals is reactive in the cytochemical procedure used to visualize microperoxisomes.

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“…Many microperoxisomes in the pigment epithelial cells appear circular or only slightly elongate (Figs. [1][2][3][4][5][6][7][8][9][10]. However, more elongate forms are encountered (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…Connock et al (5) have recently isolated microperoxisomes from homogenates of the absorptive cells of the small intestine. The isolated fraction was rich in D-amino acid oxidase activity as well as catalase activity.…”

Section: P M Leuenberger and A B Novikoff Studies On Microperoxisomesmentioning

confidence: 99%

Studies on microperoxisomes. VII. Pigment epithelial cells and other cell types in the retina of rodents.

Leuenberger¹,

Novikoff²

1975

The Journal of Cell Biology

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Peroxisomal Oxidases and Suggestions for the Mechanism of Action of Insulin and Other Hormones

Hamilton

1985

Advances in Enzymology - And Related Areas of Molecular Biology

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Stereological analysis of peroxisomes and mitochondria in intestinal epithelium of patients with peroxisomal deficiency disorders: Zellweger's syndrome and neonatal‐onset adrenoleukodystrophy

Black

Cornacchia

1986

Am. J. Anat.

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Peroxisomes, participants in lipid metabolism, have been shown to be altered in liver in two metabolic diseases in which long-chain fatty acids accumulate in tissues: Zellweger's syndrome and neonatal adrenoleukodystrophy (ALD). The intestine also plays a role in lipid metabolism, and we have had the opportunity to compare peroxisomes in normal intestinal epithelium with those from patients with Zellweger's syndrome and neonatal ALD at the electron microscopic level by using the combined techniques of cytochemistry and stereological analysis. Peroxisomes were numerous in intestinal epithelium of the normal individuals. They were ellipsoidal in shape with average diameters of 0.37 by 0.56 micron and filled with coarsely granular, DAB+ content. Peroxisomes in the intestinal epithelium of the ALD patient were similar in appearance and number but smaller in size (0.28 by 0.44 micron). Peroxisomes of normal appearance were absent from the intestinal epithelium of patients with Zellweger's syndrome; DAB+ content, however, was observed in rare, membrane-bound structures of much smaller size (0.12 by 0.19 micron). In liver of patients with Zellweger's syndrome, peroxisomes are lacking; in neonatal ALD they are abnormal in appearance and greatly reduced in number. The presence of rare minute peroxisomes in the intestinal epithelium in Zellweger's syndrome and of small peroxisomes in this epithelium in neonatal ALD indicate that peroxisomes in the intestinal epithelium are affected in these diseases, but to a lesser extent than in the liver. In the ALD intestinal epithelium, DAB+ material was also seen in long, sinuous, tubular or cisternal elements intermingled and occasionally in continuity with peroxisomes. It is suggested that these represent the early stages of peroxisome formation, the peroxisomal reticulum as originally envisioned by Lazarow, while the rare structures seen in Zellweger's represent rudiments of such a reticulum. Lamellar inclusions and clear spaces occurred in the cytoplasm adjacent to these structures indicating either that material accumulated there had been extracted during fixation or that these regions are more susceptible to autolysis. Mitochondria are also involved in lipid metabolism and have been reported to be abnormal in Zellweger's tissue. No qualitative differences were observed in the mitochondria of the intestinal epithelia examined in this study. Although quantitation revealed a greater mean volume, number, and surface density of mitochondria in the intestinal epithelia of neonatal ALD, it was not a statistically significant difference in all cases.

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A Zonal Rotor Method for the Preparation of Microperoxisomes From Epithelial Cells of Guinea Pig Small Intestine

Cited by 36 publications

References 27 publications

Studies on microperoxisomes. VII. Pigment epithelial cells and other cell types in the retina of rodents.

Studies on microperoxisomes. VII. Pigment epithelial cells and other cell types in the retina of rodents.

Peroxisomal Oxidases and Suggestions for the Mechanism of Action of Insulin and Other Hormones

Stereological analysis of peroxisomes and mitochondria in intestinal epithelium of patients with peroxisomal deficiency disorders: Zellweger's syndrome and neonatal‐onset adrenoleukodystrophy

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