Studies on microperoxisomes. VII. Pigment epithelial cells and other cell types in the retina of rodents.

Abstract

The pigment epithelial cell of the retina actively participates in two aspects of lipid metabolism: (a) the fatty acid esterification of vitamin A and its storage and transport to the photoreceptors, and (b) the phagocytosis and degradation of the lipoprotein membrane disks shed from the photoreceptor cells. Study of the pigment epithelial cells of adult albino and pigmented rodents has revealed the abundance of an organeUe, microperoxisomes, not previously known to exist in this cell type. The metabolism, transport, and storage of lipids are major functions of other cell types which possess large numbers of microperoxisomes associated with a highly developed smooth endoplasmic reticulum. Microperoxisomes were encountered, but relatively rarely, in MUller cells and vascular endothelial cells. A tubular system in photoreceptor terminals is reactive in the cytochemical procedure used to visualize microperoxisomes.

Changes in intestinal villi, cell area and intracellular autophagic vacuoles related to intestinal function in chickens

“…Nonfrozen transverse sections were cut at 30-µm using a Microslicer (Model DTK-1000, Dosaka EM Co., Japan). Thick sections were treated with an acid phosphatase staining method using a modification of the Gomori method (Leuenberger and Novikoff, 1975). Ultrathin sections were then obtained to demonstrate lysosomes in the epithelial cells using the conventional method, as described above, except without electron staining.…”

The endoplasmic reticulum: a cytochemist's view (a review).

“…(48) proposed the term melanolysosome. Maul (41) and Maul and Romsdahl (42) suggest that AcPase may be involved in degrading enzymes after melanization is completed or in degrading old melanin granules but they present no evidence for the proposals.…”

Biliary copper excretion by hepatocyte lysosomes in the rat. Major excretory pathway in experimental copper overload.

“…To have a specific morphologic marker for lysosomes, we used acid phosphatase histochemistry (27) in thin slices of liver immersed for 3 h in 2% formaldehyde (prepared from paraformaldehyde), 2.5% glutaraldehyde, in 0.1 M cacodylate buffer, pH 7.5, 0.025% CaCI2, 5% sucrose. After fixation, tissue was rinsed twice with buffer and then kept overnight in cold buffer before nonfrozen sections were prepared with a vibratome.…”