Identification of PEG-induced water stress responsive transcripts using co-expression network in Eucalyptus grandis

Dasgupta, Modhumita Ghosh; Dharanishanthi, Veeramuthu

doi:10.1016/j.gene.2017.06.050

Cited by 7 publications

(5 citation statements)

References 95 publications

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“…Contrarily, after heat stress SOD and CAT activity decreased, partially confirming the data found in Corylus avellana pollen genotypes, in which HS reduced SOD and CAT activities [39]. Interestingly, also osmotin, a multifunctional protein that is overexpressed under stress conditions [40,41] and acts as an osmoprotectant, participates in the regulation of ROS level; the protein accumulates when pollen was stressed and continues to accumulate during recovery [42,43]. In our experimental setup, ROS content in S and PRS samples was lower than in P and PR samples, indicating that probably osmotin contributes to limit their concentration.…”

Section: Discussionsupporting

confidence: 79%

Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Mareri

Faleri

Aloisi

et al. 2021

IJMS

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Global warming leads to a progressive rise in environmental temperature. Plants, as sessile organisms, are threatened by these changes; the male gametophyte is extremely sensitive to high temperature and its ability to preserve its physiological status under heat stress is known as acquired thermotolerance. This latter can be achieved by exposing plant to a sub-lethal temperature (priming) or to a progressive increase in temperature. The present research aims to investigate the effects of heat priming on the functioning of tobacco pollen grains. In addition to evaluating basic physiological parameters (e.g., pollen viability, germination and pollen tube length), several aspects related to a correct pollen functioning were considered. Calcium (Ca2+) level, reactive oxygen species (ROS) and related antioxidant systems were investigated, also to the organization of actin filaments and cytoskeletal protein such as tubulin (including tyrosinated and acetylated isoforms) and actin. We also focused on sucrose synthase (Sus), a key metabolic enzyme and on the content of main soluble sugars, including UDP-glucose. Results here obtained showed that a pre-exposure to sub-lethal temperatures can positively enhance pollen performance by altering its metabolism. This can have a considerable impact, especially from the point of view of breeding strategies aimed at improving crop species.

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Section: Discussionsupporting

confidence: 79%

Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Mareri

Faleri

Aloisi

et al. 2021

IJMS

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“…For Amrutha et al (2019), a high expression of water responsive transcripts like osmotin (OSM34) has registered in E. tereticornis and E. camaldulensis, indicating that level of osmotin can act as an indicator in screening water-responsive genotypes, added to that, dehydration responsive element binding proteins DREB/CBF, C-repeat/DRE-binding factor (CBF1c and CBF2), glutathione peroxidase (GPX6), and raffinose synthase family protein (SIP) showed also significant up-regulation. Expression of osmotin during water deficiency condition reported also in E. cladocalyx (Spokevicius et al, 2017) and E. grandis (Ghosh Dasgupta and Dharanishanthi, 2017). For instance, E. camaldulensis revealed a high genetic variation related to considerable variation in growth, wood quality and tolerance to three environmental factors: salinity, drought and frost (Yang et al, 2017).…”

Section: Molecular Molecular Molecular Molecular R R R Response Espon...mentioning

confidence: 90%

“…Moreover, other authors Bartels and Sunkar (2005); Wang et al (2006) and; Morran et al (2011) studies indicated that other elements play a pivotal role in cellular protection and stress alleviation such as dehydrins, molecular chaperones, water channel proteins, transporters and biosynthetic enzymes, growth regulators, protein phosphates, phospholipid metabolic proteins and protein kinases . In Eucalyptus, extensive studies on morpho-physiological and biochemical response to water stress condition is reported, while molecular response is limited to transcriptome-wide response in E. globulus and E. cladocalyx (Spokevicius et al, 2017), E. camaldulensis (Thumma et al, 2012), E. alba hybrid and E. urophylla × E.grandis (Villar et al, 2011), and E. grandis (Ghosh Dasgupta and Dharanishanthi, 2017). In this genus, the major transcription factors which regulate water stress include zinc finger transcription factor family protein (Villar et al, 2011), such as MYB, NAC, ERF, HB12 (Thumma et al, 2012) and HSFs, DREB2A, DEAR3 (Ghosh Dasgupta and Dharanishanthi, 2017).…”

Section: Molecular Molecular Molecular Molecular R R R Response Espon...mentioning

confidence: 99%

“…In Eucalyptus, extensive studies on morpho-physiological and biochemical response to water stress condition is reported, while molecular response is limited to transcriptome-wide response in E. globulus and E. cladocalyx (Spokevicius et al, 2017), E. camaldulensis (Thumma et al, 2012), E. alba hybrid and E. urophylla × E.grandis (Villar et al, 2011), and E. grandis (Ghosh Dasgupta and Dharanishanthi, 2017). In this genus, the major transcription factors which regulate water stress include zinc finger transcription factor family protein (Villar et al, 2011), such as MYB, NAC, ERF, HB12 (Thumma et al, 2012) and HSFs, DREB2A, DEAR3 (Ghosh Dasgupta and Dharanishanthi, 2017). For Amrutha et al (2019), a high expression of water responsive transcripts like osmotin (OSM34) has registered in E. tereticornis and E. camaldulensis, indicating that level of osmotin can act as an indicator in screening water-responsive genotypes, added to that, dehydration responsive element binding proteins DREB/CBF, C-repeat/DRE-binding factor (CBF1c and CBF2), glutathione peroxidase (GPX6), and raffinose synthase family protein (SIP) showed also significant up-regulation.…”

Section: Molecular Molecular Molecular Molecular R R R Response Espon...mentioning

confidence: 99%

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Anatomical, physiological, biochemical and molecular responses of Eucalyptus spp. under water deficit conditions and characteristics of Tunisian arid species: an overview

et al. 2022

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The genus Eucalyptus occurs in a large range of environmental conditions, mainly arid/semi-arid areas. It includes species with high capacity to survive with extremely low water potential. For that, our review shows an important effect of drought on Eucalyptus spp. plants’ resistance mechanisms and management strategies. In fact, water stress acts directly on growth, productivity, yield, it affects also response to pests and diseases, disturbs wood formation and essential oil yield. However, the general patterns of response to water stress varied among species, genotypes, hybrids and clones. To assume, reducing water loss in eucalyptus species is manifested by reducing leaves area, reducing gas exchange, increasing water uptake thanks to a prolific and deep root systems. A greater accumulation of osmolytes that gives rise to osmotic adjustment including carbohydrates especially cyclitols and quercitol, other amino acids and organic acids, also some proteins which play a vital role in sustaining cellular functions under drought conditions. More than that, water stress increases mainly the levels of pigments, chlorophyll fluorescence parameters, malondialdehyde (MDA), abscisic acid (ABA) and the biosynthesis of triacylglycerols (TAGs) in Eucalyptus species.

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“…Eucalyptus plantations are located all over the world [1]. In Brazil, most tree species used to supply the forest industry are of the genus Eucalyptus; they have favorable characteristics for the industry and can adapt to different edaphic and climatic conditions [2,3]. In the Amazon region, forest plantations with Eucalyptus are expanding, especially in pasture areas [4], where volumetric timber stock should be constantly evaluated.…”

Section: Introductionmentioning

confidence: 99%

Artificial Neural Networks and Linear Regression Reduce Sample Intensity to Predict the Commercial Volume of Eucalyptus Clones

Júnior

Rocha

Ebling

et al. 2019

Forests

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Equations to predict Eucalyptus timber volume are continuously updated, but most of them cannot be used for certain locations. Thus, equations of similar strata are applied to clonal plantations where trees cannot be felled to fit volumetric models. The objective of this study was to use linear regression and artificial neural networks (ANN) to reduce the number of trees sampled while maintaining the accuracy of commercial volume predictions with bark up to 4 cm in diameter at the top (v) of Eucalyptus clones. Two methods were evaluated in two scenarios: (a) regression model fit and ANN training with 80% of the data (533 trees) and per clone group with 80% of the trees in each group; and (b) model fit and ANN training with trees of only one clone group at ages two and three, with sample intensities of six, five, four, three, two, and one tree per diameter class. The real and predicted v averages did not differ in sample intensities from six to two trees per diameter class with different methods. The frequency distribution of individuals by volume class by the two methods (regression and ANN) compared to the real values were similar in scenarios (a) and (b) by the Kolmogorov–Smirnov test (p-value > 0.01). The application of ANN was more effective for total data analysis with non-linear behavior, without sampled environment stratification. The Prodan model also generates estimates with accuracy, and, among the regression models, is the best fit to the data. The volume with bark up to 4 cm in diameter at the top of Eucalyptus clones can be predicted with at least three trees per diameter class with regression (root mean square error in percentage, RMSE = 12.32%), and at least four trees per class with ANN (RMSE = 11.73%).

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Identification of PEG-induced water stress responsive transcripts using co-expression network in Eucalyptus grandis

Cited by 7 publications

References 95 publications

Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Anatomical, physiological, biochemical and molecular responses of Eucalyptus spp. under water deficit conditions and characteristics of Tunisian arid species: an overview

Artificial Neural Networks and Linear Regression Reduce Sample Intensity to Predict the Commercial Volume of Eucalyptus Clones

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