Identification of PDGFRα Positive Populations of Interstitial Cells in Human and Guinea Pig Bladders

Monaghan, Kevin; Johnston, Louise; McCloskey, Karen

doi:10.1016/j.juro.2012.03.117

Cited by 50 publications

(67 citation statements)

References 30 publications

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“…the area between the urothelium and detrusor smooth muscle) and IC in the detrusor [4] (inter-and intramuscular). Having been reported in bladders from different species including rat [5], guinea pig [4,6], mouse [7] and human beings [8], these two IC populations appear to be a constant, species-independent finding. Functional data on bladder IC are still limited, and the general hypothesis is that LP IC might be involved in non-neural sensory signal transduction from the urothelium towards the detrusor muscle, while detrusor IC might have a role in pacemaking and/or transduction of pacemaking signals in the detrusor area [3].…”

Section: Introductionmentioning

confidence: 79%

“…Acute administration of IM caused decreased contractility in guinea pig and human bladder [29], while chronic administration of IM in neonatal rats resulted in impaired development of networks of intermuscular IC [5]. Considering that IM also blocks PDGFRa [30], a receptor widely expressed on bladder IC [7,20], we think that the effects of IM on bladder contractility and IC development might be explained via the PDGFRa pathway. Fig.…”

mentioning

confidence: 90%

“…In guinea pig bladder, we were unable to perform decent MCT/KIT double stains, due to the absence of reliable commercially available MCT antibodies. Most publications reporting on KIT + ICC in bladder did not perform MCT/KIT double stains, with no reports of MCT/KIT double stains in guinea pig bladder [7,12,13,15,16,27] (for an overview, see also Table 2). …”

Section: Kitmentioning

confidence: 99%

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The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Gevaert¹,

Vanstreels²,

Daelemans³

et al. 2017

European Urology Supplements

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The mast/stem cell growth factor receptor KIT has long been assumed to be a specific marker for interstitial cells of Cajal (ICC) in the bladder, with possible druggable perspectives. However, several authors have challenged the presence of KIT + ICC in recent years. The aim of this study was therefore to attempt to clarify the conflicting reports on KIT expression in the bladder of human beings, rat, mouse and guinea pig and to elucidate the possible role of antibody-related issues and interspecies differences in this matter. Fresh samples were obtained from human, rat, mouse and guinea pig cystectomies and processed for single/double immunohistochemistry/immunofluorescence. Specific antibodies against KIT, mast cell tryptase (MCT), anoctamin-1 (ANO1) and vimentin were used to characterize the cell types expressing KIT. Gut (jejunum) tissue was used as an external antibody control. Our results revealed KIT expression on mast cells but not on ICC in human, rat, mouse and guinea pig bladder. Parallel immunohistochemistry showed KIT expression on ICC in human, rat, mouse and guinea pig gut, which confirmed the selectivity of the KIT antibody clones. In conclusion, we have shown that KIT + cells in human, rat, mouse and guinea pig bladder are mast cells and not ICC. The present report is important as it opposes the idea that KIT + ICC are present in bladder. In this perspective, functional concepts of KIT + ICC being involved in sensory and/or motor aspects of bladder physiology should be revised.

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Section: Introductionmentioning

confidence: 79%

mentioning

confidence: 90%

Section: Kitmentioning

confidence: 99%

See 1 more Smart Citation

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Gevaert¹,

Vanstreels²,

Daelemans³

et al. 2017

European Urology Supplements

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show abstract

“…Many of the PDGFR␣ϩ cells in the LP as well as the detrusor muscle colabeled with vimentin antibodies. PDGFR␣ϩ cells were found to be present also in human and guinea pig bladder (207). In guinea pig bladder, PDGFR␣ϩ cells had a branched stellate morphology and formed cellular networks in the lamina propria.…”

Section: Interstitial Cells/myofibroblastsmentioning

confidence: 91%

Urothelial Signaling

Birder

Andersson

2013

Physiological Reviews

388

362

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The urothelium, which lines the inner surface of the renal pelvis, the ureters, and the urinary bladder, not only forms a high-resistance barrier to ion, solute and water flux, and pathogens, but also functions as an integral part of a sensory web which receives, amplifies, and transmits information about its external milieu. Urothelial cells have the ability to sense changes in their extracellular environment, and respond to chemical, mechanical and thermal stimuli by releasing various factors such as ATP, nitric oxide, and acetylcholine. They express a variety of receptors and ion channels, including P2X3 purinergic receptors, nicotinic and muscarinic receptors, and TRP channels, which all have been implicated in urothelial-neuronal interactions, and involved in signals that via components in the underlying lamina propria, such as interstitial cells, can be amplified and conveyed to nerves, detrusor muscle cells, and ultimately the central nervous system. The specialized anatomy of the urothelium and underlying structures, and the possible communication mechanisms from urothelial cells to various cell types within the bladder wall are described. Changes in the urothelium/ lamina propria ("mucosa") produced by different bladder disorders are discussed, as well as the mucosa as a target for therapeutic interventions.

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“…Antibodies to PDGFR␣ label a distinct population of cells with the muscle layers of the gastrointestinal tract and bladder (7,19,24,29,31,35). These cells are distinct from interstitial cells of Cajal but occupy similar anatomical niches in mice, nonhuman primates, and humans.…”

mentioning

confidence: 99%

A novel population of subepithelial platelet-derived growth factor receptor α-positive cells in the mouse and human colon

Kurahashi

Nakano

Peri

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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Identification of PDGFRα Positive Populations of Interstitial Cells in Human and Guinea Pig Bladders

Cited by 50 publications

References 30 publications

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Urothelial Signaling

A novel population of subepithelial platelet-derived growth factor receptor α-positive cells in the mouse and human colon

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