A novel population of subepithelial platelet-derived growth factor receptor α-positive cells in the mouse and human colon

Kurahashi, Masaaki; Nakano, Yasuko; Peri, Lauren E.; Townsend, Jared; Ward, Sean M.; Sanders, Kenton M.

doi:10.1152/ajpgi.00001.2013

Cited by 69 publications

(91 citation statements)

References 45 publications

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“…Moreover, in this study, and in several others, it was demonstrated that none of the CD34 and PDGFRα + cells were c-Kit labeled, definitively excluding that these cells are ICC (50)(51)(52)(53)(54)(55) (Figure 4). Notably, while in these reports (54,55) some cells located in the axes of the villi were PDGFRα + , Vannucchi et al (51) could not find any CD34 positive cells at this level. Under TEM, cells with the features of TC were described in the axes of the villi and called myoid cells (56)(57)(58).…”

Section: Tc Identification By Immunohistochemistry (See Table 1)supporting

confidence: 67%

“…In humans, although all the CD34 positive cells were found to be also PDGFRα + , in the axes of the villi there were described only as PDGFRα + cells (51,54,55,63). In the muscle wall of humans and rodents the PDGFRα + cells also expressed the SK3 channel (see above) but, in the mouse, these PDGFRα/SK3 + cells were CD34 negative (60).…”

Section: Tc Subtypesmentioning

confidence: 98%

“…Recently, by using the PDGFRα antibody, cells sharing the same distribution of the CD34 positive cells were identified (51)(52)(53)(54)(55). The PDGF/PDGF receptor signaling pathway plays critical roles in mammalian organogenesis and murine GI villous morphogenesis, and it has been demonstrated that selective inhibition of the PDGFR suppresses longitudinal smooth muscle differentiation (53).…”

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

“…To note, some authors have considered the PDGFRα + cells to correspond to the FLC (52, 53, 60). However, because of the vagueness of this indirect definition of the cell identity, also, this name has been gradually abandoned and in the most recent papers these cells are simply called PDGFRα + cells (54,55,(61)(62)(63). Several groups of researcher have shown that in the gut of rodents and humans the PDGFRα + cells also express the small conductance Ca 2+ -activated K + channel 3 (SK3) (53,55,(60)(61)(62).…”

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

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Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Vannucchi

Traini

2016

Biomolecular Concepts

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Abstract:In the interstitium of the connective tissue several types of cells occur. The fibroblasts, responsible for matrix formation, the mast cells, involved in local response to inflammatory stimuli, resident macrophages, plasma cells, lymphocytes, granulocytes and monocytes, all engaged in immunity responses. Recently, another type of interstitial cell, found in all organs so far examined, has been added to the previous ones, the telocytes (TC). In the gut, in addition to the cells listed above, there are also the interstitial cells of Cajal (ICC), a peculiar type of cell exclusively detected in the alimentary tract with multiple functions including pace-maker activity. The possibility that TC and ICC could correspond to a unique cell type, where the former would represent an ICC variant outside the gut, was initially considered, however, further studies have clearly shown that ICC and TC are two distinct types of cells. In the gut, while the features and the roles of the ICC are established, part of the scientific community is still disputing these 'new' interstitial cells to which several names such as fibroblast-like cells (FLCs), interstitial Cajal-like cells or, most recently, PDGFRα + cells have been attributed. This review will detail the main features and roles of the TC and ICC with the aim to establish their relationships and hopefully define the identity of the TC in the gut.

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Section: Tc Identification By Immunohistochemistry (See Table 1)supporting

confidence: 67%

Section: Tc Subtypesmentioning

confidence: 98%

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

See 2 more Smart Citations

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Vannucchi

Traini

2016

Biomolecular Concepts

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“…Recently, a new class of fibroblastlike cells expressing platelet derived growth factor receptor α (PDGFRα) have been described in the subepithelial layer of the gastrointestinal tract [38] . As has been demonstrated in both the murine detrusor muscle and colon, these cells express P2Y 1 receptors coupled to SK channels, leading to purinergic inhibition [39][40][41] .…”

Section: Intracellular Signaling Cascade Following Purinergic Receptomentioning

confidence: 99%

P2Y receptor-mediated transient relaxation of rat longitudinal ileum preparations involves phospholipase C activation, intracellular Ca2+ release and SK channel activation

et al. 2016

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Aim: Purinergic signaling plays a major role in the enteric nervous system, where it governs gut motility through a number of P2X and P2Y receptors. The aim of this study was to investigate the P2Y receptor-mediated motility in rat longitudinal ileum preparations. Methods: Ileum smooth muscle strips were prepared from rats, and fixed in an organ bath. Isometric contraction and relaxation responses of the muscle strips were measured with force transducers. Drugs were applied by adding of stock solutions to the organ bath to yield the individual final concentrations. Results: Application of the non-hydrolyzable P2 receptor agonists α,β-Me-ATP or 2-Me-S-ADP (10, 100 µmol/L) dose-dependently elicited a transient relaxation response followed by a sustained contraction. The relaxation response was largely blocked by SK channel blockers apamin (500 nmol/L) and UCL1684 (10 µmol/L), PLC inhibitor U73122 (100 µmol/L), IP 3 receptor blocker 2-APB (100 µmol/L) or sarcoendoplasmic Ca 2+ ATPase inhibitor thapsigargin (1 µmol/L), but not affected by atropine, NO synthase blocker L-NAME or tetrodotoxin. Furthermore, α,β-Me-ATP-induced relaxation was suppressed by P2Y 1 receptor antagonist MRS2179 (50 µmol/L) or P2Y 13 receptor antagonist MRS2211 (100 µmol/L), and was abolished by co-application of the two antagonists, whereas 2-Me-S-ADP-induced relaxation was abolished by P2Y 6 receptor antagonist MRS2578 (50 µmol/L). In addition, P2Y 1 receptor antagonist MRS2500 (1 µmol/L) not only abolished α,β-Me-ATP-induced relaxation, but also suppressed 2-Me-S-ADP-induced relaxation. Conclusion: P2Y receptor agonist-induced transient relaxation of rat ileum smooth muscle strips is mediated predominantly by P2Y 1 receptor, but also by P2Y 6 and P2Y 13 receptors, and involves PLC, IP 3 , Ca 2+ release and SK channel activation, but is independent of acetylcholine and NO release.

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Role of platelet‐derived growth factor‐α in eosinophilic and non‐eosinophilic chronic rhinosinusitis with nasal polyps

Lin

Xiong

et al. 2014

Int Forum Allergy Rhinol

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A novel population of subepithelial platelet-derived growth factor receptor α-positive cells in the mouse and human colon

Abstract: Sanders KM. A novel population of subepithelial platelet-derived growth factor receptor ␣-positive cells in the mouse and human colon.

Cited by 69 publications

References 45 publications

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

P2Y receptor-mediated transient relaxation of rat longitudinal ileum preparations involves phospholipase C activation, intracellular Ca2+ release and SK channel activation

Role of platelet‐derived growth factor‐α in eosinophilic and non‐eosinophilic chronic rhinosinusitis with nasal polyps

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