Identification of PCH1 Eyespot Resistance Gene in the Collection of Wheat Lines (Triticum Aestivum L.)

Kwiatek, Michał; Pankiewicz, Katarzyna; Wiśniewska, Halina; Korbas, M.; Danielewicz, Jakub

doi:10.2478/v10045-012-0040-5

Cited by 5 publications

(4 citation statements)

References 14 publications

(20 reference statements)

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“…Resistance expression was evaluated using inoculation tests made on 100 randomly chosen plants from each of four replications by calculating the infection index (K) and the percentage of infected stems according to Kwiatek et al (2012;2015). A sample of 50 plants from each replicate of each wheat genotype and the control varieties were evaluated (a total of 200 stems for each genotype).…”

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confidence: 99%

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Kwiatek

Wiśniewska

Korbas

et al. 2016

Journal of Plant Protection Research

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Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.

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confidence: 99%

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Kwiatek

Wiśniewska

Korbas

et al. 2016

Journal of Plant Protection Research

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“…Therefore, in this study, we used two more recent markers Xorw1 and Xorw5, which are more accurate in identifying the presence of a Pch1 gene because they are completely linked to Ep-D1 and Pch1 (Leonard et al 2008). Effectiveness of the markers selected for the identification of the Pch1 gene in winter wheat varieties in the presented study had already been reported by other researchers as well as in our previous research (Groenewald et al 2003;Santra et al 2006;Kwiatek et al 2012;Burt et al 2014;Kwiatek et al 2015Kwiatek et al , 2016.…”

Section: Resultsmentioning

confidence: 53%

“…The level of the leaf sheath infection was measured using a I -IV scale: I -no symptoms; II -less than 50% of leaf sheath surface infected; IIIover 50% of leaf sheath surface infected; IV -100% of leaf sheath surface infected, rotten tissue. The results were presented as a mean from each replication according to the K-index which was calculated according to the formula (Kwiatek et al 2012):…”

Section: Ssr Marker Analysismentioning

confidence: 99%

Journal of Plant Protection Research

Majka¹,

Kwiatek²,

Korbas³

et al. 2018

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Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2 gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines: DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2 genes was identified, where the association between the Pch1 and the locus of the Xorw5 marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did not significantly affect the grain yield (from the plot), although the yield was highest in the presence of both genes. A significant effect of the presence of the Pch1 gene on thousand kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher TKW values than lines without both genes or with the Pch2 gene only.

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“…In Poland only eight wheat breeding lines completely resistant or highly resistant to infection by Oculimacula acuformis and O. yallundae causing true eyespot were bred. All these cultivars have Pch1 resistance gene (Kwiatek et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of winter Triticale Cultivars to Rhizoctonia Cerealis (sharp eyespot) and R. Solani

Lemańczyk¹

2012

Journal of Plant Protection Research

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Abstract:In the field study period from 2006 to 2010, the incidence and severity of sharp eyespot caused by Rhizoctonia were recorded on 36 cultivars of triticale at the milk ripe growth stage. Four localities in north-central Poland were included: Chrząstowo, Dębina, Kończewice and Minikowo. The susceptibility of the seedlings of 30 cultivars of triticale to R. cerealis (AG-D subgroup I) and R. solani (AG-5) was studied in the laboratory. There was much variation in incidence and severity of sharp eyespot between years and locations. The disease was most intense at Chrząstowo. At this location, the mean percentage of diseased stems on 28 cultivars was Most symptoms developed on coleoptiles, with less symptoms on the leaves and the least on the roots. There was much variation in susceptibility of triticale cultivars to both Rhizoctonia species. Cultivars were grouped into six categories according to the intensity of seedling infection. Categories 1, 2 and 3, representing low, moderate and high susceptibility to R. cerealis, included 17, 10 and 3 cultivars, respectively. Categories 4, 5 and 6, representing low, moderate and high susceptibility to R. solani, included 3, 12 and 15 cultivars, respectively. Cultivars Baltiko and Zorro had low, and cv. Cultivo had high susceptibility to both Rhizoctonia species. No cultivar was resistant to Rhizoctonia. There was a positive correlation between infection by R. cerealis and R. solani. Infection of coleoptiles by R. cerealis or R. solani was significantly correlated with infection of leaves. No correlation between intensity of sharp eyespot on triticale plants in the field and on seedlings in controlled conditions was found.

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Identification of PCH1 Eyespot Resistance Gene in the Collection of Wheat Lines (Triticum Aestivum L.)

Cited by 5 publications

References 14 publications

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Journal of Plant Protection Research

Susceptibility of winter Triticale Cultivars to Rhizoctonia Cerealis (sharp eyespot) and R. Solani

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