Identification of pathological grade and prognosis‐associated lncRNA for ovarian cancer

Abstract

Ovarian carcinoma (OC) is one of the most common malignant tumors in female genitals. In recent years, the therapeutic effect of OC has been significantly improved through the application of effective chemotherapy regimen. However, the 5‐year survival rate is also lower than 30% due to high rate of relapse. So, it is needed to screen reliable predictive and prognostic markers of OC. Ovarian cancer gene expression data and corresponding clinical data used were downloaded from Gene Expression Omnibus database. Weighted gene expression network analysis (WGCNA) and Cox proportional hazards regression (PHR) were used to screen Pathological Grade and Prognosis‐associated long noncoding RNA (lncRNA). Kaplan‐Meier analysis and receiver operating characteristic curves analysis were performed to evaluate the predictive ability of the selected lncRNA. Gene Ontology (GO) enrichment and Gene Set Enrichment Analysis (GSEA) enrichment analysis methods were used to explore the possible mechanisms of the selected lncRNA affecting the development of OC. Five reliably lncRNAs (LINC00664, LINC00667, LINC01139, LINC01419, and LOC286437) was identified through a series of bioinformatics methods. In testing cohorts, we found that the five lncRNAs in predicting the risk of OC recurrence is robustness, and multivariate Cox PHR analysis indicate that the five lncRNAs is an independent risk factor for OC recurrence. Moreover, GO and GSEA enrichment analysis showed that the five lncRNAs are involved in multiple ovarian cancer occurrence mechanism. In summary, all these findings indicated that the five lncRNAs can effectively predict the risk of recurrence of ovarian cancer.

Significance of tumor mutation burden combined with immune infiltrates in the progression and prognosis of ovarian cancer

“…The main treatment method is a combination of tumor cell ablation and chemotherapy drugs, such as paclitaxel and platinum-based drugs. Despite the development of diagnostic and treatment technology, the mortality rate has not improved significantly [3]. Therefore, searching for new treatment methods plays an important role in improving the prognosis of patients with OC.…”

Long noncoding RNAs, ENST00000598996 and ENST00000524265, are correlated with favorable prognosis and act as potential tumor suppressors in bladder cancer

“…To date, a considerable proportion of these DE-lncRNAs have been reported in multiple carcinomas. For example, lncRNA XIST (ENST00000429829) ( 36 , 37 ), TINCR (ENST00000448587) ( 38 , 39 ), MEG3 (ENST00000398460, ENST00000398461 and ENST00000452120) ( 40 , 41 ), FENDRR (ENST00000593604 and ENST00000598996) ( 42 , 43 ), and ADAMTS9-AS2 (ENST00000460833, ENST00000474768 and ENST00000481312) ( 44 , 45 ) were observed in gastric, lung and breast cancers; LINC01419 (ENST00000522365) in esophageal ( 46 ) and ovarian ( 47 ) carcinomas; JHDM1D-AS1(ENST00000566699) in pancreatic cancer ( 48 ); MIR205HG (ENST00000433108) in cervical cancer ( 49 ); MBNL1-AS1 (ENST00000445466) ( 50 ) and PART1 (ENST00000515734) ( 51 ) in non-small cell lung cancer. MAGI2-AS3 (ENST00000452320) was observed in hepatocellular carcinoma ( 52 ).…”

Identification of genes associated with cancer stem cell characteristics in head and neck squamous cell carcinoma through co‐expression network analysis

“…Relative quantitative method was used to detect the expression of target genes and GAPDH was used as internal reference. 15…”