Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis

Lo, Wan Yu; Tsai, Ming Hsui; Tsai, Yuhsin; Hua, Chun Hung; Tsai, Fuu Jen; Huang, Shiuan Yi; Tsai, Chang Hai; Lai, Chien‐Chen

doi:10.1016/j.cca.2006.06.030

Cited by 123 publications

(95 citation statements)

References 43 publications

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“…It has been implicated that down-regulation of HSP 27 may confer to chemo-resistance associated with cellular growth delay [27]. Consistent with our findings of up-regulated HSP 27 in tonsillar cancer, other investigations on oral cancer have found a similar expression pattern of this protein [3,17,18].…”

Section: Discussionsupporting

confidence: 81%

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Proteomic analysis of protein expression in human tonsillar cancer differentially expressed proteins characterize human tonsillar cancer

Roblick¹,

Bader

Hammarstedt

et al. 2008

Acta Oncologica

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Section: Discussionsupporting

confidence: 81%

“…It is known that high expression of tubulins in tumor cells is associated with poor response to chemotherapy and a shorter progression-free survival [16]. To date, proteomics-based studies in head and neck cancer did not report expression changes in these proteins [2,3,17,18].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of protein expression in human tonsillar cancer differentially expressed proteins characterize human tonsillar cancer

Roblick¹,

Bader

Hammarstedt

et al. 2008

Acta Oncologica

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“…24,25 While its chaperone activity and its contribution to myopathies, gliopathies and neurodegenerative diseases have been well documented, 24,25 the molecular understanding of αB-crystallin's role in cancer is only beginning to emerge. High levels of αB-crystallin expression have been reported in various cancer types, including prostate cancer, 26 oral squamous cell carcinomas, 27 renal cell carcinomas, 26,28 basal-like breast carcinomas 29 and gliomas. [30][31][32] Expectedly, αB-crystallin possesses various tumorigenesis-relevant activities, such as transforming, anti-apoptotic, and invasive properties.…”

Section: αB-crystallin Is a Novel Gliomagenic Oncoproteinmentioning

confidence: 99%

What drives intense apoptosis resistance and propensity for necrosis in glioblastoma? A role for Bcl2L12 as a multifunctional cell death regulator

Stegh¹,

Chin²,

Louis³

et al. 2008

Cell Cycle

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Glioblastoma (GBM) is the most common type of primary brain cancer and carries a dismal prognosis primarily due to the emergence of resistance towards extant radiation, conventional and targeted chemotherapies. Although GBM resists therapy-induced apoptosis, tumors show a seemingly paradoxical propensity for florid intratumoral necrogenesis. This necrosis manifests pathologically as microscopic foci or confluent expanses of necrotic tumor. While it is now well recognized that necrosis is an active cell death process and that apoptosis and necrosis death modalities are intertwined on multiple levels, the precise molecular mechanisms and genetic elements underlying these forms of cell death in GBM remain areas of active investigation. In recent oncogenomic studies, we identified a novel GBM oncoprotein, Bcl2-Like 12 (Bcl2L12), which is significantly expressed in the majority of primary GBM tumor specimens and distantly related to canonical Bcl-2 proteins. Due to its distinctive impact on cell death signaling, Bcl2L12 phenocopies pro-necrotic and anti-apoptotic propensities of highgrade glioma: Mechanistically, we determined that unlike prototypic Bcl-2 family members, Bcl2L12 does not safeguard mitochondrial membrane integrity, but instead potently inhibits apoptosis at the level of post-mitochondrial effector caspase-3/7 activation. A combination of enforced expression, RNAi-mediated extinction, co-localization and protein interaction studies revealed that Bcl2L12 inhibits caspases 3 and 7 via distinct mechanisms. Direct physical interaction underlies Bcl2L12's inhibition of caspase-7 processing, whereas Bcl2L12-induced transcriptional upregulation of the small heat shock protein αB-crystallin is instrumental to neutralization of caspase-3 activation. Mirroring the cellular phenotype elicited by energy depletion, genetic or pharmacologic inhibition of post-mitochondrial apoptosis signaling molecules, Bcl2L12 promotes necrogenesis in glial cells in the context of a proapoptotic stimulus establishing that it represents a novel regulator of the balance between apoptosis and necrosis in GBM.

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“…Proteins are the main participators involved in the different metabolic processes both in the tumor pathogenesis, growth, metastasis, and after simulation by physical and chemical factors [6]. So the changes of proteins expression are always related to how the simulated metabolic process happen and develop.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induced by low-intensity ultrasound in vitro: Alteration of protein profile and potential molecular mechanism

Feng¹,

Wan²

2017

AIP Conference Proceedings

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Abstract. To analyze the potential mechanism related to the apoptosis induced by low intensity focused ultrasound, comparative proteomic method was introduced in the study. After ultrasound irradiation (3.0 W/cm 2 , 1 minute, 6 hours incubation post-irradiation), the human SMMC-7721 hepatocarcinoma cells were stained by trypan blue to detect the morphologic changes, and then the percentage of early apoptosis were tested by the flow cytometry with double staining of FITC-labelled Annexin V/Propidium iodide. Two-dimensional SDS polyacrylamide gel electrophoresis was used to get the protein profile and some proteins differently expressed after ultrasound irradiation were identified by MALDI-TOF mass spectrometry. It's proved early apoptosis of cells were induced by low intentisy focused ultrasound. After ultrasound irradiation, the expressing characteristics of several proteins changed, in which protein p53 and heat shock proteins are associated with apoptosis initiation. It is suggested that the low-intensity ultrasound-induced apoptotic cancer therapy has the potential application via understanding its relevant molecular signaling and key proteins. Moreover, the comparative proteomic method is proved to be useful to supply information about the protein expression to analyze the metabolic processes related to bio-effects of biomedical ultrasound.

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Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis

Cited by 123 publications

References 43 publications

Proteomic analysis of protein expression in human tonsillar cancer differentially expressed proteins characterize human tonsillar cancer

Proteomic analysis of protein expression in human tonsillar cancer differentially expressed proteins characterize human tonsillar cancer

What drives intense apoptosis resistance and propensity for necrosis in glioblastoma? A role for Bcl2L12 as a multifunctional cell death regulator

Apoptosis induced by low-intensity ultrasound in vitro: Alteration of protein profile and potential molecular mechanism

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