Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa

Noguchi, Rieko; Banno, Shinpei; Ichikawa, Ryota; Fukumori, Fumiyasu; Ichiishi, Akihiko; Kimura, Makoto; Yamaguchi, Isamu; Fujimura, Masatake

doi:10.1016/j.fgb.2006.08.003

Cited by 81 publications

(90 citation statements)

References 46 publications

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“…This regulation would allow the clock to prepare cells for osmotic stress that occurs at sunrise and the osmosensing system to activate the same pathway when there is unexpected osmotic stress. This mechanism may exist in all eukaryotes, because both stress-induced p38 MAPKs and circadian clocks are conserved from fungi to humans (4,22,23,(30)(31)(32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

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Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Vitalini

Paula

Goldsmith

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks are composed of central oscillators, input pathways that transduce external information to the oscillators, and output pathways that allow the oscillators to temporally regulate cellular processes. Little is known about the output pathways. In this study, we show that the Neurospora crassa osmosensing MAPK pathway, essential for osmotic stress responses, is a circadian output pathway that regulates daily rhythms in the expression of downstream genes. Rhythmic activation of the highly conserved stress-activated p38-type MAPK [Osmotically Sensitive-2 (OS-2)] by the N. crassa circadian clock allows anticipation and preparation for hyperosmotic stress and desiccation that begin at sunrise. These results suggest a conserved role for MAPK pathways in circadian rhythmicity.circadian output pathway ͉ circadian rhythm ͉ Neurospora crassa ͉ osmotic stress phosphorelay

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Section: Discussionmentioning

confidence: 99%

“…Many homologous genes are regulated by the OS-2 MAPK in N. crassa (33). Thus, rhythms in the activity of the OS pathway would likely influence the expression of a number of downstream genes through control of OS-2-regulated transcription factors.…”

Section: Discussionmentioning

confidence: 99%

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Vitalini

Paula

Goldsmith

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…The pathway has been characterized most completely in yeast (O'Rourke et al, 2002), and is found in numerous fungal species on the basis of comparative genomics (Krantz et al, 2006). In N. crassa, a histidine kinase sensor OS-1 (Alex et al, 1996;Miller et al, 2002) and an associated response regulator RRG-1 (Jones et al, 2007) function upstream of the MAPKKK OS-4 and MAPKK OS-5 (Fujimura et al, 2003), and MAPK OS-2 (Zhang et al, 2002), to elicit both glycerol accumulation (Noguchi et al, 2007) and ion accumulation (Lew et al, 2006) during turgor recovery after hyperosmotic shock.…”

Section: Introductionmentioning

confidence: 99%

Transient responses during hyperosmotic shock in the filamentous fungus Neurospora crassa

Lew

Nasserifar

2009

Microbiology

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Fungal cells maintain an internal hydrostatic pressure (turgor) of about 400-500 kPa. In the filamentous fungus Neurospora crassa, the initial cellular responses to hyperosmotic treatment are loss of turgor, a decrease in relative hyphal volume per unit length (within 1 min) and cell growth arrest; all recover over a period of 10-60 min due to increased net ion uptake and glycerol production. The electrical responses to hyperosmotic treatment are a transient depolarization of the potential (within 1 min), followed by a sustained hyperpolarization (after 4 min) to a potential more negative than the initial potential (a driving force for ion uptake). The nature of the transient depolarization was explored in the context of other transient responses to hyperosmotic shock, to determine whether activation of a specific ion permeability or some other rapid change in electrogenic transport was responsible. Changing the ionic composition of the extracellular medium revealed that K + permeability increases and H + permeability declines during the transient depolarization. We suggest that these changes are due to concerted inhibition of the electrogenic H + -ATPase, and an increase in a K + conductance. Knockout mutants of known K + (tok, trk, trm-8, hak-1) and Cl " (a clc-3 homologue) channels and transporters had no effect on the transient depolarization, but trk and hak-1 do play a role in osmoadaptation, as does a homologue of a serine kinase regulator of H + -ATPase in yeast, Ptk2.

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“…Three basic MAPK modules have been identified, but, so far, only the kinase cascade homologous to the S. cerevisiae osmosensing/stress pathway has been fully characterized in the filamentous ascomycetes N. crassa and Aspergillus nidulans (Zhang et al 2002;Fujimura et al 2003;Jones et al 2007;Noguchi et al 2007). 1 Osmostress signaling in N. crassa is transduced through the OS1 histidine kinase to the OS4, OS5, and OS2 MAPK cascade.…”

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confidence: 99%

“…os mutants are unable to grow on high-osmolarity media and are resistant to phenylpyrrole fungicides. Furthermore, lysis and increased pigmentation of asexually derived spores (macroconidia) and female sterility due to the lack of protoperithecia has been reported, yet the cellular or developmental defects involved have not been analyzed in depth (Zhang et al 2002;Fujimura et al 2003;Jones et al 2007;Noguchi et al 2007). A. nidulans HOG pathway mutants are similarly growth inhibited under high-osmolarity conditions and are sensitive to oxidative stress (Kawasaki et al 2002;Furukawa et al 2005).…”

mentioning

confidence: 99%

The Nuclear Dbf2-Related Kinase COT1 and the Mitogen-Activated Protein Kinases MAK1 and MAK2 Genetically Interact to Regulate Filamentous Growth, Hyphal Fusion and Sexual Development in Neurospora crassa

et al. 2008

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Ndr kinases, such as Neurospora crassa COT1, are important for cell differentiation and polar morphogenesis, yet their input signals as well as their integration into a cellular signaling context are still elusive. Here, we identify the cot-1 suppressor gul-4 as mak-2 and show that mutants of the gul-4/mak-2 mitogen-activated protein (MAP) kinase pathway suppress cot-1 phenotypes along with a concomitant reduction in protein kinase A (PKA) activity. Furthermore, mak-2 pathway defects are partially overcome in a cot-1 background and are associated with increased MAK1 MAPK signaling. A comparative characterization of N. crassa MAPKs revealed that they act as three distinct modules during vegetative growth and asexual development. In addition, common functions of MAK1 and MAK2 signaling during maintenance of cell-wall integrity distinguished the two ERK-type pathways from the p38-type OS2 osmosensing pathway. In contrast to separate functions during vegetative growth, the concerted activity of the three MAPK pathways is essential for cell fusion and for the subsequent formation of multicellular structures that are required for sexual development. Taken together, our data indicate a functional link between COT1 and MAPK signaling in regulating filamentous growth, hyphal fusion, and sexual development.

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Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa

Cited by 81 publications

References 46 publications

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Transient responses during hyperosmotic shock in the filamentous fungus Neurospora crassa

The Nuclear Dbf2-Related Kinase COT1 and the Mitogen-Activated Protein Kinases MAK1 and MAK2 Genetically Interact to Regulate Filamentous Growth, Hyphal Fusion and Sexual Development in Neurospora crassa

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