Identification of orcokinins in single neurons in the stomatogastric nervous system of the crayfish, <i>Cherax destructor</i>

Skiebe, Petra; Dreger, Mathias; Meseke, Maurice; Evers, Jan Felix; Hucho, Ferdinand

doi:10.1002/cne.10145

Cited by 49 publications

(58 citation statements)

References 34 publications

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“…This is reminiscent of work on some other modulators that are also present in STG somata in young animals (Fénelon et al, 1998Kilman et al, 1999). There are now a number of reports of STG somata stained for one or another neuromodulator Skiebe, 1999;Li et al, 2002;Skiebe et al, 2002). In these studies, as in this paper, STG somatic staining is relatively weak compared with either neuropil staining or staining of somata elsewhere in the nervous system.…”

Section: Discussionmentioning

confidence: 67%

Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

Pulver

Thirumalai

Richards

et al. 2003

J of Comparative Neurology

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Dopamine and histamine are neuromodulators found in the adult stomatogastric nervous system (STNS) of several crustacean species. We used antibodies against tyrosine hydroxylase (TH) and histamine to map the distribution and developmental acquisition of the dopamine and histamine neurons in the STNS of the lobster, Homarus americanus. Embryos, larvae, juvenile and adult animals were studied. TH labeling was present in the STNS as early as E80-85 (80-85% of embryonic development). A subset of preparations in embryos, larvae, juveniles, and adults contained 1-5 labeled somata in the stomatogastric ganglion. Histamine staining appeared in the STNS as early as E50. The distribution of both TH and histamine staining remained relatively constant through development. Electrophysiological recordings demonstrated that receptors for both amines are present in the embryo. Bath application of dopamine increased the frequency of the pyloric rhythm in embryos, and evidence for dopaminergic activation of peripherally initiated spiking in motor axons was seen. In embryos and adults, histamine inhibited the motor patterns produced by the stomatogastric ganglion (STG). These data suggest that the dopaminergic and histaminergic systems in H. americanus appear relatively early in development and that the effects of each are largely maintained through development.

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Section: Discussionmentioning

confidence: 67%

Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

Pulver

Thirumalai

Richards

et al. 2003

J of Comparative Neurology

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“…However, in the crustacean stomatogastric system, no growth factors are presently known, and only one peptidergic neuromodulator has been shown to be produced by neurons within the STG. However, this modulator is not produced by neurons of the pyloric network itself (Skiebe et al 2002), and it is thus unlikely to act as "activity messenger" in the system.…”

Section: Discussionmentioning

confidence: 99%

Activity and Neuromodulatory Input Contribute to the Recovery of Rhythmic Output After Decentralization in a Central Pattern Generator

Zhang

Khorkova

Rodríguez

et al. 2009

Journal of Neurophysiology

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Zhang Y, Khorkova O, Rodriguez R, Golowasch J. Activity and neuromodulatory input contribute to the recovery of rhythmic output after decentralization in a central pattern generator. J Neurophysiol 101: 372-386, 2009. First published July 2, 2008 doi:10.1152/jn.01290.2007. Central pattern generators (CPGs) are neuronal networks that control vitally important rhythmic behaviors including breathing, heartbeat, and digestion. Understanding how CPGs recover activity after their rhythmic activity is disrupted has important theoretical and practical implications. Previous experimental and modeling studies indicated that rhythm recovery after central neuromodulatory input loss (decentralization) could be based entirely on activity-dependent mechanisms, but recent evidence of long-term conductance regulation by neuromodulators suggest that neuromodulator-dependent mechanisms may also be involved. Here we examined the effects of altering activity and the neuromodulatory environment before decentralization of the pyloric CPG in Cancer borealis on the initial phase of rhythmic activity recovery after decentralization. We found that pretreatments altering the network activity through shifting the ionic balance or the membrane potential of pyloric pacemaker neurons reduced the delay of recovery initiation after decentralization, consistent with the recovery process being triggered already during the pretreatment period through an activity-dependent mechanism. However, we observed that pretreatment with neuromodulators GABA and proctolin, acting via metabotropic receptors, also affected the initial phase of the recovery of pyloric activity after decentralization. Their distinct effects appear to result from interactions of their metabotropic effects with their effects on neuronal activity. Thus we show that the initial phase of the recovery process can be accounted for by the existence of distinct activity-and neuromodulator-dependent pathways. We propose a computational model that includes activity-and neuromodulator-dependent mechanisms of the activity recovery process, which successfully explains the experimental observations and predicts the results of key biological experiments.

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“…This antibody has been used previously to map the distribution of orcokinins in the nervous system of numerous crustacean species (Bungart et al, 1994;Dircksen et al, 2000;Li et al, 2002;Pulver and Marder, 2002;Skiebe et al, 2002Skiebe et al, , 2003. To confirm its specificity for orcokininrelated peptides in C. productus, we preincubtaed the antibody with 10 -6 M orcokinin (Bachem; catalog No.…”

Section: Whole-mount Immunohistochemistry and Imagingmentioning

confidence: 99%

Hormone complement of the Cancer productus sinus gland and pericardial organ: An anatomical and mass spectrometric investigation

Fu¹,

Kutz²,

Schmidt³

et al. 2005

J of Comparative Neurology

130

167

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In crustaceans, circulating hormones influence many physiological processes. Two neuroendocrine organs, the sinus gland (SG) and the pericardial organ (PO), are the sources of many of these compounds. As a first step in determining the roles played by hemolymph-borne agents in the crab Cancer productus, we characterized the hormone complement of its SG and PO. We show via transmission electron microscopy that the nerve terminals making up each site possess dense-core and/or electron-lucent vesicles, suggesting diverse complements of bioactive molecules for both structures. By using immunohistochemistry, we show that small molecule transmitters, amines and peptides, are among the hormones present in these tissues, with many differentially distributed between the two sites (e.g., serotonin in the PO but not the SG). With several mass spectrometric (MS) methods, we identified many of the peptides responsible for the immunolabeling and surveyed the SG and PO for peptides for which no antibodies exist. By using MS, we characterized 39 known peptides [e.g., beta-pigment-dispersing hormone (beta-PDH), crustacean cardioactive peptide, and red pigment-concentrating hormone] and de novo sequenced 23 novel ones (e.g., a new beta-PDH isoform and the first B-type allatostatins identified from a non-insect species). Collectively, our results show that diverse and unique complements of hormones, including many previously unknown peptides, are present in the SG and PO of C. productus. Moreover, our study sets the stage for future biochemical and physiological studies of these molecules and ultimately the elucidation of the role(s) they play in hormonal control in C. productus.

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Identification of orcokinins in single neurons in the stomatogastric nervous system of the crayfish, Cherax destructor

Cited by 49 publications

References 34 publications

Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

Activity and Neuromodulatory Input Contribute to the Recovery of Rhythmic Output After Decentralization in a Central Pattern Generator

Hormone complement of the Cancer productus sinus gland and pericardial organ: An anatomical and mass spectrometric investigation

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