Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

Abstract

Dopamine and histamine are neuromodulators found in the adult stomatogastric nervous system (STNS) of several crustacean species. We used antibodies against tyrosine hydroxylase (TH) and histamine to map the distribution and developmental acquisition of the dopamine and histamine neurons in the STNS of the lobster, Homarus americanus. Embryos, larvae, juvenile and adult animals were studied. TH labeling was present in the STNS as early as E80-85 (80-85% of embryonic development). A subset of preparations in embryos, larvae, juveniles, and adults contained 1-5 labeled somata in the stomatogastric ganglion. Histamine staining appeared in the STNS as early as E50. The distribution of both TH and histamine staining remained relatively constant through development. Electrophysiological recordings demonstrated that receptors for both amines are present in the embryo. Bath application of dopamine increased the frequency of the pyloric rhythm in embryos, and evidence for dopaminergic activation of peripherally initiated spiking in motor axons was seen. In embryos and adults, histamine inhibited the motor patterns produced by the stomatogastric ganglion (STG). These data suggest that the dopaminergic and histaminergic systems in H. americanus appear relatively early in development and that the effects of each are largely maintained through development.

Hormone complement of the Cancer productus sinus gland and pericardial organ: An anatomical and mass spectrometric investigation

“…The epitope recognized by the tyrosine hydroxylase antibody has been mapped by its manufacturer to the middle portion of the protein, where extensive species homology exists. Consequently, this antibody is thought to be broadly cross-reactive with homologous proteins from a variety of species, including those of several decapod crustaceans (Tierney et al, 1999(Tierney et al, , 2003Pulver and Marder, 2002;Pulver et al, 2003;Fort et al, 2004). To assess directly the specificity of the dopamine antibody, preincubation of the antibody with either dopamine-BSA conjugate or unconjugated BSA was undertaken.…”

Histaminergic signaling in the central nervous system of Daphnia and a role for it in the control of phototactic behavior

“…22939; Hudson, WI, USA), and has been used previously to map the distribution of histamine in the nervous systems of a number of other crustacean species (e.g. Mulloney and Hall, 1991;Le Feuvre et al, 2001;Pulver et al, 2003;Christie et al, 2004;Fu et al, 2005;Hartline and Christie, 2010). Visualization of the histamine antibody was accomplished using an Alexa-Fluor-488-conjugated donkey antirabbit IgG (catalog no.…”

“…Mulloney and Hall, 1991;Le Feuvre et al, 2001;Pulver et al, 2003;Christie et al, 2004;Fu et al, 2005;Hartline and Christie, 2010), ours is its first use in daphnids. Thus, to increase our confidence in the specificity of the histamine-like immunoreactivity described above, antibody adsorption controls were conducted (Table1).…”

Modulation of an Integrated Central Pattern Generator–Effector System: Dopaminergic Regulation of Cardiac Activity in the Blue CrabCallinectes sapidus

“…4A, arrow). This cell corresponds to a dopaminergic neuron, termed the "L-cell" (Selverston et al 1976), that has been described in a number of crustacean species, including the crab Carcinus maenas , the lobsters Panulirus interruptus (Kushner and Maynard 1977), Homarus gammarus (Cournil et al 1984(Cournil et al , 1994, Homarus americanus (Pulver et al 2003;Siwicki et al 1987), and the crayfish FIG. 4. Tyrosine hydroxylase-like immunoreactivity (THli) in the L-cells and their projections.…”