Identification of Oklahoma Isolate as a Strain of Pseudomonas pseudomallei

Abstract

Based on the morphological, physiological, biochemical, and nutritional characteristics, cellular fatty acid and lipid composition, ubiquinone-8 as the major respiratory quinone, guanine-plus-cytosine content of DNA, DNA-DNA homology value, and sequence alignment of 16S rRNA nucleotides, Oklahoma isolate was reidentified as a strain of Pseudomonas pseudomallei.

Burkholderia oklahomensis sp. nov., a Burkholderia pseudomallei-like species formerly known as the Oklahoma strain of Pseudomonas pseudomallei

“…The initial study indicated that the Oklahoma strain may represent a variant of B. pseudomallei or potentially a novel species based on the results of biochemical assays, guinea pig inoculations, fatty acid analysis and a fluorescent antibody test (McCormick et al, 1977). Subsequent studies have produced conflicting interpretations regarding the identification of the Oklahoma strain as a strain of B. pseudomallei (Godoy et al, 2003;Tomaso et al, 2004Tomaso et al, , 2005Yabuuchi et al, 1992a). Yabuuchi et al (1992a) performed a variety of standard microbiological tests as well as DNA-DNA hybridization and 16S rRNA gene sequencing with the clinical isolate of the Oklahoma strain and concluded that it was a strain of B. pseudomallei.…”

“…A BLAST query of GenBank on 1 September 2005, using the OK 16S rRNA gene consensus sequence, did not yield an exact match to any 16S rRNA gene sequence in the database (Altschul et al, 1990). Although Yabuuchi et al (1992a) stated that the 16S rRNA gene sequence for the Oklahoma strain was identical to the 16S rRNA gene sequence for B. pseudomallei, the sequence was not included in their paper, nor was an entry available in GenBank.…”

“…A BESTFIT analysis comparing the concatenated sequences of ST81 with ST51, the ST of the type strain of B. pseudomallei, indicated a match of 95 %. When Yabuuchi et al (1992a) performed DNA-DNA hybridization in their study, they concluded that the Oklahoma clinical isolate did not represent a novel species. However, they made this determination by using a lower threshold of species delineation (i.e.…”

“…Subsequent studies have produced conflicting interpretations regarding the identification of the Oklahoma strain as a strain of B. pseudomallei (Godoy et al, 2003;Tomaso et al, 2004Tomaso et al, , 2005Yabuuchi et al, 1992a). Yabuuchi et al (1992a) performed a variety of standard microbiological tests as well as DNA-DNA hybridization and 16S rRNA gene sequencing with the clinical isolate of the Oklahoma strain and concluded that it was a strain of B. pseudomallei. Recently, Godoy et al (2003) completed a study using multilocus sequence typing (MLST) that indicated that the Oklahoma isolates were divergent from all other B. pseudomallei isolates in their study panel and suggested that the Oklahoma strain may be a novel species.…”

Pseudomonas aeruginosa under Phosphorus-Poor Culture Conditions: Phospholipid-Poor Bacterial Membranes, and Susceptibility to Antibacterial Chemicals, High Temperature and Low pH.

“…The second development for two-dimensional TLC was carried out in solvent system II (chloroform-methanol-water [65:23:4, vol/vol]). Spots on the plates were visualized with 0.3% ninhydrin-buthanolacetic acid solution 31) , a-naphtol reagent 38) , Dittmer reagent 8) , or 50% H 2 SO 4 and identified by referring to standard substances and data in previous reports 36,37) . For analyses of fatty acids and amino acids of OLs, the lipid purified from a spot on a TLC plate was acid-hydrolyzed, and the n-hexane-soluble fraction and HCl-soluble fraction were examined by high pressure liquid chromatography (HPLC) as described previously 24,26) .…”