Burkholderia oklahomensis sp. nov., a Burkholderia pseudomallei-like species formerly known as the Oklahoma strain of Pseudomonas pseudomallei

Abstract

C6786, the clinical isolate of the ‘Oklahoma’ strain of Pseudomonas (now Burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in Oklahoma, USA. Environmental isolates C7532 and C7533 from the Oklahoma accident site were found to match C6786. These three isolates and a clinical isolate originally identified as B. pseudomallei that was recovered from a person in Georgia, USA, involved in an automobile accident were characterized by biochemical, 16S rRNA gene sequencing, multilocus sequence typing and DNA–DNA hybridization analyses. Results indicated that these strains comprise a novel species. The name Burkholderia oklahomensis sp. nov. is proposed, with strain C6786T (=LMG 23618T=NCTC 13387T=CCUG 51349T) as the type strain.

Pneumonia and Septicemia Caused by Burkholderia thailandensis in the United States

“…CDC3015869 and CDC2721121 were identical by MLST and were designated sequence type (ST) 101 (7,8). Strain 82172 was previously designated ST 73, and although it did not share any alleles with the six STs of B. thailandensis previously identified, it clustered most closely with B. thailandensis based on analysis of the concatenated sequences of the MLST loci (8).…”

“…Although sequencing of the 16S rRNA gene can reveal differences among Burkholderia species, MLST is based upon differences in the sequences of seven housekeeping genes and has been shown to be not only useful for subtyping strains of B. pseudomallei but also capable of resolving genetic differences among close relatives of B. pseudomallei (6,7,8). CDC3015869 and CDC2721121 were identical by MLST and were designated sequence type (ST) 101 (7,8).…”

Novel Pan-Genomic Analysis Approach in Target Selection for Multiplex PCR Identification and Detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia Complex Species: a Proof-of-Concept Study

“…Previous attempts to identify organisms in the B. pseudomallei group, i.e., B. pseudomallei, B. thailandensis, Burkholderia mallei, and Burkholderia oklahomensis (19), included sequencing of the groEL gene (48), the 16S rRNA gene (17), and various housekeeping genes by multilocus sequence typing (MLST) schemes (20,38). Methods that do not require gene sequencing included probe-based real-time PCR and loopmediated isothermal amplification targeting Burkholderia type III secretion system genes (10,30,40), single-nucleotide polymorphism (SNP) typing (42), and multiplex PCR amplification of a region flanking variable copies of a repetitive element (RE) (25).…”

A Review of Melioidosis Cases in the Americas

“…28 Two of the cases (one case reported from Oklahoma in 1976 and the other reported from Georgia in 1979) have since been reclassified as infections with the closely related bacteria Burkholderia oklahomensis. 29 An additional 84 cases reported in that series occurred in soldiers/veterans with a history of service in Vietnam. Another review reported more than 340 cases among U.S. soldiers with service to Vietnam.…”