Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity against Fusarium moniliforme

Jian, Jiang; Gao, Ling; Bie, Xiaomei; Lu, Zhaoxin; Liu, Hongxia; Zhang, Chong; Lü, Fang; Zhao, Haizhen

doi:10.1186/s12866-016-0645-3

Cited by 79 publications

(49 citation statements)

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“…SPE70, SPE80, SPE90 and SPE100: fraction eluted with 70, 80, 90 and 100% methanol from SPE C 18 and the same letter means do not differ statistically (P < 0Á05) by Duncan tests. strong antifungal activity against Fusarium graminearum (Gong et al 2015), Botryosphaeria dothidea (Li et al 2016) and Botrytis cinerea (Zhang et al 2013), among others; surfactin analogues demonstrate high levels of suppressive activity against Fusarium oxysporum (Gu et al 2017), Fusarium moniliforme (Jiang et al 2016;Sarwar et al 2018), R. solanacearum (Xiong et al 2015), among others. In this study, the LP mixture (iturin, fengycin and surfactin) yielded by B. amyloliquefaciens strain FJAT-2349 could effectively inhibit the growth of R. solanacearum.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

et al. 2019

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Aims The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT‐2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. Methods and Results Lipopeptides were extracted by acid precipitation and resolved in methanol and their structure was identified through LC‐QTOF‐MS/MS method. The antibacterial activities of the LPs were evaluated through inhibition zone experiments. The biocontrol efficiency of the LPs against tomato bacterial wilt was examined by a pot test. The LPs were composed of iturin (C14–C17 iturin A), fengycin (C14/C16 fengycin A, C14 fengycin B2, C16 fengycin A2/B2, C16–C17 fengycin B, C15 fengycin A derivatives and C15 fengycin B derivatives) and surfactin (C12–C16 surfactin A). Moreover, the composition of the LPs was significantly influenced by the culture medium and temperature; the contents of iturin, fengycin and surfactin varied within the range from 0·41–5·89, 4·54–181·67 and 2·05–19·65 mg l−1 in the different culture media and from 0·39–11·04, 1·45–215·14 and 7·79–24·43 mg l−1 under different culture temperatures respectively. The results indicated that the contents of the LP mixture, fengycin and surfactin secreted from FJAT‐2349 all decreased along with an increasing culture temperature. The fermentation supernatants and LP extracts had the strongest inhibition activities of Ralstonia solanacearum when strain FJAT‐2349 was incubated at 25°C using potato dextrose broth as the culture medium among all the assayed culture conditions. The purified fengycin was found to be the active antibacterial compound against R. solanacearum, but the purified surfactin was not. The pot experiments demonstrated that the LPs secreted from the strain FJAT‐2349 could effectively control the tomato bacterial wilt with a biocontrol efficiency of 97·6%. Conclusions The LPs secreted from strain FJAT‐2349 could serve as potential biocontrol agents against tomato bacterial wilt. Significance and Impact of the Study The LPs exhibited good potential applications in the biocontrol of tomato bacterial wilt.

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

et al. 2019

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“…The culture was centrifuged (10,000 × g, 4 °C) for 10 min to remove LC. Then, 6 N HCL was added to adjust the pH of the supernatant (pH=2.0) and induce precipitation(42). Finally, the CLPs contained in the precipitation were fully dissolved in methanol, and a rotary evaporator (RE52CS-1, YARONG, Shanghai, China) was then used at 50°C and 65 rpm to obtain CLE for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Wang

Zhang

et al. 2019

Preprint

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15Potato late blight triggered by Phytophthora infestans ((Mont.) de Bary) represents a 16 great food security threat worldwide and is difficult to control. Currently, Bacillus spp. 17 have been considered biocontrol agents to control many fungal diseases. Here, 18 Bacillus subtilis WL-2 was selected as the antifungal strain with the most potential 19 against P. infestans mycelium growth. Additionally, the functional metabolites 20 extracted from WL-2 were identified as IturinA-family cyclic lipopeptides (CLPs) via 21 2 high-performance liquid chromatography (HPLC) and electrospray ionization mass 22 spectrometry (ESI-MS). Analyses using scanning and transmission electron 23 microscopy (SEM and TEM) revealed that IturinA caused a change in the mycelial 24 surface and damage to the internal cell structure, including cell membrane disruption 25 and irregular organelle formation. Moreover, propidium iodide staining and nucleic 26 acid and protein release were detected to clarify the cell membrane damage caused by 27 IturinA. Additionally, IturinA triggered reactive oxygen species (ROS) generation and 28 malondialdehyde (MDA) production. Mitochondrial membrane potential (MMP), 29 mitochondrial respiratory chain complexes activity (MRCCA), respiratory control rate 30 (RCR), and oxidative phosphorylation efficiency (P/O) assays indicated that P. 31 infestans mitochondria affected by IturinA were so seriously damaged that the MMP 32 and MRCCA declined remarkably and that mitochondrial ATP production ability was 33 weakened. Therefore, IturinA induces cell membrane damage, oxidative stress, and 34 dysfunction of mitochondria, resulting in P. infestans hyphal cell death. As such, the 35 results highlight that B. subtilis WL-2 and IturinA have great potential as candidates for 36 inhibiting P. infestans mycelium growth and controlling potato late blight. 37 IMPORTANCE 38Potato (Solanum tuberosum L.) is the fourth most common global food crop, and 39 its planting area and yield increase yearly. Notably, in 2015, China initiated a potato 40 staple food conversion strategy, and by 2020, approximately 50% of potatoes will 41 be consumed as a staple food. The plant pathogen fungus Phytophthora infestans 42 ((Mont.) de Bary) is the culprit of potato late blight; however, biological agents 43 3 rather than chemicals are highly necessary to control this threatening disease. In 44 this study, we discovered an antifungal substance, IturinA, a lipopeptide produced 45 by Bacillus subtilis WL-2. Moreover, our research revealed the actual mechanism 46 of IturinA against P. infestans mycelium growth and clarified the potential of B. 65Behind rice, wheat, and corn, potato (Solanum tuberosum L.) is the fourth most 66 stable food crop in the world. However, potato production is often endangered by 67 many pathogens. Worryingly, late blight triggered by Phytophthora infestans ((Mont.) 68 de Bary) could directly reduce or even eliminate potato production, and an outbreak 69 of this disease could even result in a grievous ...

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“…corresponding to C [14][15][16] Bacillomycin L, surfactin A and fengycin A were both proved to have the strong antifungal activity against a wide variety of fungi, especially for filamentous fungi (Ongena and Jacques 2008;Falardeau et al 2013;Jiang et al 2016). The three antifungal metabolites from LHSB1 could be responsible for its antifungal activity against S. rolfsii.…”

Section: Bacillus Coagulans Strain Nbrc 12583 (Nr_041523)mentioning

confidence: 99%

Seed‐borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii

et al. 2019

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Aims This study aimed to obtain an antagonistic endophyte against Sclerotium rolfsii from peanut seeds, evaluate the biocontrol efficacy towards peanut stem rot and explore its antifungal mechanism against S. rolfsii. Methods and Results Thirty‐seven endophytic bacteria were isolated from peanut seeds, six of which exhibited stronger antagonistic activities against S. rolfsii (inhibition rate, IR of hyphae growth ≥70%). Strain LHSB1, the strongest antagonistic strain, was identified as Bacillus velezensis. LHSB1 showed 93·8% of radial growth inhibition of S. rolfsii hyphae and exhibited obvious antagonistic activity against another six pathogenic fungi of peanut. Pot experiments showed two different LHSB1 treatments both significantly reduced the disease incidence and severity of stem rot (P < 0·05) compared to the controls, and the biocontrol efficacy reached 62·6–70·8%, significantly higher than that of Carbendazim control (P < 0·05). Further analyses revealed LHSB1 culture filtrate significantly inhibited sclerotia formation and germination, caused the abnormalities and membrane integrity damage of S. rolfsii hyphae, which might be the possible mode of action of LHSB1 against S. rolfsii. Three antifungal lipopeptides bacillomycin A, surfactin A and fengycin A, were detected in LHSB1 culture extracts by UPLC‐ESI‐MS, which could be responsible for the biocontrol activity of LHSB1 against S. rolfsii. Conclusion Our results suggested that the seed‐borne endophytic B. velezensis LHSB1 would be a tremendous potential agent for the biocontrol of peanut stem rot caused by S. rolfsii. Significance and Impact of the Study This comprehensive study provides a candidate endophytic biocontrol strain and reveals its antifungal mechanism against S. rolfsi. To the best of our knowledge, this is the first time that seed‐borne endophytic B. velezensis was used as the biocontrol agent to control peanut stem rot.

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Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity against Fusarium moniliforme

Cited by 79 publications

References 39 publications

Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Seed‐borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii

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