Identification of novel serum peptides biomarkers for female breast cancer patients in Western China

Yang, Juan; Xiong, Xiaofan; Liu, Siyuan; Zhu, Jiang; Luo, Mai; Liu, Liying; Zhao, Lingyu; Qin, Yannan; Song, Taek Lyul; Huang, Chen

doi:10.1002/pmic.201500321

Cited by 34 publications

(20 citation statements)

References 26 publications

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“…Mass spectrometry (MS) combined with software-generated models can be used to compare the proteomic profiles of cancer patients and healthy individuals quickly and accurately [ [4] , [5] , [6] ]. In the past few years, there have been many reports of the development of diagnosis or prognosis models and screening of new peptide biomarkers of CRC, however, these models and biomarkers have not been applied to clinical practice due to variation in sensitivity and specificity [ [7] , [8] , [9] , [10] , [11] ].…”

Section: Introductionmentioning

confidence: 99%

Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients

et al. 2018

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BackgroundThere is an urgent need for the identification of new, clinically useful biomarkers of CRC to enhance diagnostic and prognostic capabilities.MethodsWe performed proteomic profiling on serum samples from paired pre- and post-operative CRC patients, colorectal polyps patients and healthy controls using an approach combining magnetic bead-based weak cation exchange and matrix-assisted laser desorption ionization-time of flight mass spectrometry. We next performed liquid chromatography-electrospray ionization-tandem mass spectrometry to identify the proteins and selected potential biomarker based on bioinformatics analysis of the TCGA and GEO dataset. We examined SETD7 expression in serum and tissue samples by ELISA and immunohistochemistry respectively and explored the biological function of SETD7 in vitro.Findings85 differentially expressed peptides were identified. Five peptides showing the most significant changes in abundance across paired pre- and post-operation CRC patients, colorectal polyps patients and healthy controls were identified as peptide regions of FGA, MUC5AC and SETD7. Bioinformatics analysis suggested that the up-regulation of SETD7 in CRC is relatively specific. Validation studies showed that SETD7 expression increased from healthy controls to those with colorectal polyps and finally CRC patients, and decreased after surgery. The sensitivity and specificity of SETD7 were 92.17% and 81.08%, with a high diagnostic value (AUC = 0.9477). In addition, SETD7 expression was significantly correlated with tumor stage and microsatellite instability. Knockdown of SETD7 inhibited cancer cell proliferation, induced G1/S cell cycle arrest and increased apoptosis.InterpretationOur data indicate that SETD7 could serve as a potential diagnostic and prognostic biomarker for CRC.

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Section: Introductionmentioning

confidence: 99%

Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients

et al. 2018

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“…Such, the BC patients and healthy controls were compared for differences in serum proteome via SELDI-TOF-MS, and identified that apolipoprotein CI and complement C3a desArg were up-regulated in sera of patient set ( Chung et al, 2014 , Opstal-van Winden et al, 2011 ). In order to estimate the discriminatory power of putative markers sufficiently, the investigators added benign disease controls comprising of breast fibroadenoma patients to study sets ( Meng et al, 2011 , Yang et al, 2015 ). Furthermore, the diagnostic accuracy of potential serum biomarkers were also evaluated with the multivariate logistic regression analysis ( Lumachi et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Salivary Glycopatterns as Potential Biomarkers for Screening of Early-Stage Breast Cancer

Qiao

et al. 2018

EBioMedicine

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ObjectiveWe systematically investigated and assessed the alterations of salivary glycopatterns and possibility as biomarkers for diagnosis of early-stage breast cancer.DesignAlterations of salivary glycopatterns were probed using lectin microarrays and blotting analysis from 337 patients with breast benign cyst or tumor (BB) or breast cancer (I/II stage) and 110 healthy humans. Their diagnostic models were constructed by a logistic stepwise regression in the retrospective cohort. Then, the performance of the diagnostic models were assessed by ROC analysis in the validation cohort. Finally, a double-blind cohort was tested to confirm the application potential of the diagnostic models.ResultsThe diagnostic models were constructed based on 9 candidate lectins (e.g., PHA-E + L, BS-I, and NPA) that exhibited significant alterations of salivary glycopatterns, which achieved better diagnostic powers with an AUC value > 0.750 (p < 0.001) for the diagnosis of BB (AUC: 0.752, sensitivity: 0.600, and specificity: 0.835) and I stage breast cancer (AUC: 0.755, sensitivity: 0.733, and specificity: 0.742) in the validation cohort. The diagnostic model of I stage breast cancer exhibited a high accuracy of 0.902 in double-blind cohort.ConclusionsThis study could contribute to the screening for patients with early-stage breast cancer based on precise alterations of salivary glycopatterns.

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“…There are a number of reasons for fibrinogen upregulation, of which malignant tumor accounts for 20% based on a Chinese article. Previous studies indicate that the serum level of FGA is upregulated in many tumors, such as gastric cancer, breast cancer, nasopharyngeal carcinoma, acute lymphocytic leukemia, esophageal squamous cell carcinoma, and renal cell cancer (31)(32)(33)(34)(35). The abnormal expression of FGG was also reported in hepatocellular carcinoma and ovarian cancer (36).…”

Section: Discussionmentioning

confidence: 91%

Proteomic Analysis of Cerebrospinal Fluid From Patients With Extranodal NK-/T-Cell Lymphoma of Nasal-Type With Ethmoidal Sinus Metastasis

Zeng

Zhao

et al. 2020

Front. Oncol.

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Objective: Extranodal natural killer /T-cell lymphoma (ENKTL) is an aggressive and unusual subtype of non-Hodgkin lymphoma (NHL) that it is related with the Epstein-Barr virus (EBV). CSF is considered as an ideal source of high-concenrtation disease-related proteins. We aimed at identifying the proteomic markers changes of CSF in ENKTL patients and used such changes to diagnose ENKTL. Materials and methods: In this study, CSF samples were acquired from hospitalization patients from the Cancer Center of West China Hospital, Chengdu, China. Comparative proteomic profiling are commonly used to do label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). And in this study the same method was used to characterize the variety of proteins in ENKTL patients and none-ENKTL people. Results: In the aggregate, 421 non-excrescent and functional proteins were identified among the samples. Of these proteins, 45 proteins quantified match the involved criteria. HRG, TIMP-1, SERPINA3, FGA, FGG, TF, FGB, APP, and AGT were significantly up-regulated. Discussion: We discovered that some proteins were significantly up-regulated. Also, these proteins themselves or with others proteins may be potential markers to diagnose ENKTL. The changes of proteomics may be a potential method to precisely identify the pathogenesis of the ENKTL.

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Identification of novel serum peptides biomarkers for female breast cancer patients in Western China

Cited by 34 publications

References 26 publications

Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients

Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients

Salivary Glycopatterns as Potential Biomarkers for Screening of Early-Stage Breast Cancer

Proteomic Analysis of Cerebrospinal Fluid From Patients With Extranodal NK-/T-Cell Lymphoma of Nasal-Type With Ethmoidal Sinus Metastasis

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