Identification of novel polar aryl hydrocarbon receptor agonists accumulated in liver of black-tailed gulls in Korea using advanced effect-directed analysis

Cha, Jihyun; Hong, Seongjin; Gwak, Jiyun; Mun-Gi, Kim; Lee, Jung-Hyun; Kim, Taewoo; Han, Gi Myung; Hong, Sang Hee; Hur, Jin; Giesy, John P.; Khim, Jong Seong

doi:10.1016/j.jhazmat.2022.128305

Cited by 6 publications

(32 citation statements)

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“…The reliability, reproducibility, and sensitivity of the H4IIE- luc bioassay were evaluated by confirming the dose–response relationship of the positive control (BaP), which showed consistency with previous studies (Table S4). , Luminescence activities were measured by using a Victor multilabel plate reader (PerkinElmer, Waltham, MA). The luminescence values obtained from all dosed samples are expressed in relative luminescence units (RLU).…”

Section: Methodsmentioning

confidence: 99%

“…The detailed instrumental conditions for LC-QTOFMS are presented in Table S5. AhR agonist candidates were selected through a five-step process. ,, The information about the selected candidates is presented in Table S6. Among these, standard materials for 11 AhR agonist candidates were commercially available.…”

Section: Methodsmentioning

confidence: 99%

“…3 A recent report identified several polar aryl hydrocarbon receptor (AhR) agonists, such as canrenone, mepanipyrim, medroxyprogesterone, rutaecarpine, genistein, tretinoin, etofenprox, [10]-gingerol, and eupatilin, in livers of black-tailed gulls from South Korea. 4 These rater polar compounds demonstrated significant biomagnification potential in the marine food webs. 4 The AhR is a transcription factor that can be activated by various ligands.…”

Section: ■ Introductionmentioning

confidence: 99%

“…4 These rater polar compounds demonstrated significant biomagnification potential in the marine food webs. 4 The AhR is a transcription factor that can be activated by various ligands. 5 It plays a role in regulating diverse biological processes, including xenobiotic metabolism, immune function, developmental toxicity, and cancer induction, by promoting the expression of target genes upon binding to specific DNA sequences in the nucleus.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The fin whale ( Balaenoptera physalus ) inhabits oceans ranging from polar to tropical regions and primarily feeds on small fish, squid, and crustaceans . Top predators, such as dolphins and whales, possess characteristics including wide mobility, diverse prey sources, low metabolic activity, and great lipid content. , These unique features make them valuable as sentinels for biomonitoring environmental pollutants in aquatic environments. , Long-term exposure to various environmental pollutants can have adverse effects on the health of top predators and contribute to increased environmental stress in the marine ecosystem . However, only a limited number of studies have investigated the biological effects of environmental pollutants and the presence of unknown toxicants in cetaceans.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Mid-Polar and Polar AhR Agonists in Cetaceans from Korean Coastal Waters: Application of Effect-Directed Analysis with Full-Scan Screening

Cha,

Hong,

Lee

et al. 2023

Environ. Sci. Technol.

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Major aryl hydrocarbon receptor (AhR) agonists were identified in extracts of blubber, liver, and muscle from six long-beaked common dolphins (Delphinus capensis) and one fin whale (Balaenoptera physalus) collected from Korean coastal waters using effect-directed analysis. Results of the H4IIE-luc bioassay indicated that the polar fractions of blubber and liver extracts from the fin whale exhibited relatively high AhR-mediated potencies. Based on full-scan screening with high-resolution mass spectrometry, 37 AhR agonist candidates, spanning four use categories: pharmaceuticals, pesticides, cosmetics, and natural products, were selected. Among these, five polar AhR agonists were newly identified through toxicological confirmation. Concentrations of polar AhR agonists in cetaceans were tissue-specific, with extracts of blubber and liver containing greater concentrations than muscle extracts. Polar AhR agonists with great log K OA values (>5) were found to biomagnify in the marine food chain potentially. Polar AhR agonists contributed 8.9% of the observed AhR-mediated potencies in blubber and 49% in liver. Rutaecarpine and alantolactone contributed significantly to the total AhR-mediated potencies of blubber, whereas hydrocortisone was a major AhR contributor in the liver of the fin whale. This study is the first to identify the tissue-specific accumulation of polar AhR agonists in blubber and liver extracts of cetaceans.

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Section: Methodsmentioning

confidence: 99%