Identification of novel pathogenicity-related cellulase genes in Xanthomonas oryzae pv. oryzae

Temuujin, Ulambayar; Kim, Jae-Won; Kim, Jong-Kun; Lee, Byoung-Moo; Kang, Hee‐Wan

doi:10.1016/j.pmpp.2011.08.004

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…Although no catalytic chemical activity has been identified for expansin modules, it is significant that the knockout of CelXoB renders Xanthomonas oryzae pv. oryzae KACC 10331 avirulent [67]. Another emerging non-catalytic GH5 subfamily is closely related to subfamily GH5_8.…”

Section: Resultsmentioning

confidence: 99%

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5)

et al. 2012

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BackgroundThe large Glycoside Hydrolase family 5 (GH5) groups together a wide range of enzymes acting on β-linked oligo- and polysaccharides, and glycoconjugates from a large spectrum of organisms. The long and complex evolution of this family of enzymes and its broad sequence diversity limits functional prediction. With the objective of improving the differentiation of enzyme specificities in a knowledge-based context, and to obtain new evolutionary insights, we present here a new, robust subfamily classification of family GH5.ResultsAbout 80% of the current sequences were assigned into 51 subfamilies in a global analysis of all publicly available GH5 sequences and associated biochemical data. Examination of subfamilies with catalytically-active members revealed that one third are monospecific (containing a single enzyme activity), although new functions may be discovered with biochemical characterization in the future. Furthermore, twenty subfamilies presently have no characterization whatsoever and many others have only limited structural and biochemical data. Mapping of functional knowledge onto the GH5 phylogenetic tree revealed that the sequence space of this historical and industrially important family is far from well dispersed, highlighting targets in need of further study. The analysis also uncovered a number of GH5 proteins which have lost their catalytic machinery, indicating evolution towards novel functions.ConclusionOverall, the subfamily division of GH5 provides an actively curated resource for large-scale protein sequence annotation for glycogenomics; the subfamily assignments are openly accessible via the Carbohydrate-Active Enzyme database at http://www.cazy.org/GH5.html.

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Section: Resultsmentioning

confidence: 99%

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5)

et al. 2012

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“…Novel pathogenicity-related genes including eglXoA, which encodes endoglucanase, have been isolated and characterized [14]. Whole genome sequence information of Xoo KACC10331 is available on NCBI's GenBank.…”

Section: Discussionmentioning

confidence: 99%

“…EglXoA was classified into cellulase family 5, which exhibits endo-1,4-glucanase activities, and had identity over 88.8% to endoglucanase genes, egl1 from X. campestris pv. vesicatoria [14].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, genes encoding CWDEs such as cellulase and xylanase are thought to play a role in the virulence of Xoo [12,14,29]. It has been reported that purified cellulase and lipase proteins induce defense responses in rice that are suppressible by Xoo in a T3SS-dependent manner [22].…”

Section: Discussionmentioning

confidence: 99%

“…Pathogenicity assays were performed on 60-days-old leaves of a susceptible rice cultivar (Milyang 23) by the leaf-punching method by Temujin et al [14]. Pathogenicity was observed at 14 days post inoculation.…”

Section: Pathogenicity Assaysmentioning

confidence: 99%

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Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

Temuujin¹,

Hw²

2017

J Plant Pathol Microbiol

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Symbiotic interactions of culturable microbes with the nickel hyperaccumulator Berkheya coddii and the herbivorous insect Chrysolina clathrata

et al. 2012

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Identification of novel pathogenicity-related cellulase genes in Xanthomonas oryzae pv. oryzae

Cited by 5 publications

References 28 publications

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5)

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5)

Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

Symbiotic interactions of culturable microbes with the nickel hyperaccumulator Berkheya coddii and the herbivorous insect Chrysolina clathrata

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