Identification of novel paraben-binding peptides using phage display

Targeted Killing of Staphylococcus aureus Using Specific Peptides Displayed on Yeast Vacuoles

“… Escherichia coli ER2738 host strains were provided by the Ph.D. peptide display cloning system. Staphylococcus aureus (ATCC 6538) and Saccharomyces cerevisiae 2805 (ATCC 208280) were provided by the Korean Research Institute of Bioscience and Biotechnology (KRIBB; Daejeon, South Korea) and grown in YPD medium (5 g/L yeast extract, 10 g/L tryptone, 10 g/L sodium chloride) in a 250-mL flask at 37°C and 180 rpm ( 25 , 26 ). Other bacterial strains, including Salmonella enterica serovar Choleraesuis ATCC 10708, S. Enteritidis ATCC 13076, S. enterica serovar Typhimurium KTCC 2053, S. enterica serovar Dublin ATCC 15480, Shigella sonnei ATCC 25931, Shigella flexneri KCTC 2517, E. coli BL21(DE3), Corynebacterium glutamicum ATCC 13032, and Vibrio fischeri ATCC 49387, were provided by the Department of Microbiology at Chungbuk National University (South Korea) and grown in LB medium (10 g/L yeast extract, 20 g/L peptone, 20 g/L glucose) in a 250-mL flask at 30°C and 180 rpm.…”

“…For ELISA or sequencing of the plaque, strain ER2738 was cultured and then diluted in LB medium (1:100) before mixing with the blue plaque collected from the plate. The tube was incubated at 37°C with shaking for 4.5 to 5 h and then placed in the microcentrifuge at 14,000 rpm for 30 s. After that, 80% of the supernatant was transferred to a fresh tube as the amplified phage stock ( 25 , 26 ).…”

Natural Compounds with Antimicrobial Properties in Cosmetics

“…Unfortunately, they can cause side effects such as hormone disruption via their mimicry effect on the endocrine system, diseases such as cancer, thyroid disorders, skin allergies, or even reproduction and neurological problems [ 4 ]. In addition, residues of these compounds present in domestic wastewater pollute freshwater, which disrupts aquatic [ 5 ] ecosystems. Therefore, new effective and low-toxicity preservative compounds that have no side effects on animal and human organisms or the environment are being sought.…”