Identification of novel elements which regulate the cell-type specificity of Dictyostelium 7E gene expression

Seager, Jonathan H.J.; Stevens, Bryn; McCaffery, Ian; Hames, B.D.

doi:10.1046/j.1432-0436.2001.068001022.x

Cited by 6 publications

(4 citation statements)

References 47 publications

(62 reference statements)

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“…Interestingly, a very similar sequence within the promoter of the 7E gene, CATCACC, is essential for expression in pstA cells and for repressing prestalk expression in prespore cells (Seager et al, 2001). However, we did not analyse the R1 element functionally.…”

Section: Discussionmentioning

confidence: 99%

TheDictyosteliumbZIP transcription factor DimB regulates prestalk-specific gene expression

et al. 2006

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The ecmA gene is specifically expressed in prestalk cells and its transcription is induced by the chlorinated hexaphenone DIF-1. We have purified a novel bZIP transcription factor, DimB, by affinity chromatography on two spatially separated ecmA promoter fragments. Mutagenesis of the cap-site proximal DimB-binding site (the -510 site) greatly decreases ecmA expression in the pstO cells, which comprise the rear half of the prestalk zone, and also in the Anterior-Like Cells, which lie scattered throughout the prespore region. However, DimB is not essential for normal expression of the ecmA gene, instead it spatially limits its expression; ecmA is relatively highly expressed in the subset of prestalk cells that coats the prestalk zone, but in slugs of a DimB-null strain, ecmA is highly expressed throughout the prestalk zone. Because the -510 site is required for correct ecmA expression, we posit a separate activator protein that competes with DimB for binding to the -510 site. DimB rapidly accumulates in the nucleus when cells are exposed to DIF-1, and ChIP analysis shows that, in the presence of extracellular cAMP, DIF-1 causes DimB to associate with the ecmA promoter in vivo. Thus, DIF-1 regulates DimB activity to generate a gradient of ecmA expression in the prestalk zone of the slug.

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Section: Discussionmentioning

confidence: 99%

TheDictyosteliumbZIP transcription factor DimB regulates prestalk-specific gene expression

et al. 2006

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“…) and 7E (Seager et al . ; Shimada et al . ), significantly lower expression was found in the cudA null strain as observed in the STATa null strain (Figs B, S7; Fig.…”

Section: Resultsmentioning

confidence: 99%

Regulation of ecmF gene expression and genetic hierarchy among STATa, CudA, and MybC on several prestalk A‐specific gene expressions in Dictyostelium

et al. 2016

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STATa, a Dictyostelium homologue of metazoan signal transducer and activator of transcription, is important for the organizer function in the tip region of the migrating Dictyostelium slug. We previously showed that ecmF gene expression depends on STATa in prestalk A (pstA) cells, where STATa is activated. Deletion and sitedirected mutagenesis analysis of the ecmF/lacZ fusion gene in wild-type and STATa null strains identified an imperfect inverted repeat sequence, ACAAATANTATTTGT, as a STATa-responsive element. An upstream sequence element was required for efficient expression in the rear region of pstA zone; an element downstream of the inverted repeat was necessary for sufficient prestalk expression during culmination. Band shift analyses using purified STATa protein detected no sequence-specific binding to those ecmF elements. The only verified upregulated target gene of STATa is cudA gene; CudA directly activates expL7 gene expression in prestalk cells. However, ecmF gene expression was almost unaffected in a cudA null mutant. Several previously reported putative STATa target genes were also expressed in cudA null mutant but were downregulated in STATa null mutant. Moreover, mybC, which encodes another transcription factor, belonged to this category, and ecmF expression was downregulated in a mybC null mutant. These findings demonstrate the existence of a genetic hierarchy for pstA-specific genes, which can be classified into two distinct STATa downstream pathways, CudA dependent and independent. The ecmF expression is indirectly upregulated by STATa in a CudAindependent activation manner but dependent on MybC, whose expression is positively regulated by STATa.

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“…The literature search has resulted in 5 known TREs as follows. We collected Psp-3B variant Harwood [38], ras S-promoter [39], Gene 7E psp-AT type [40], ecmB Repressor [41], and MMF Elements [42]. We found these 5 known TREs in simulation results.…”

Section: Simulation Resultsmentioning

confidence: 99%

A New Approach to Predicting Regulatory Elements in Dictyostelium Gene Expression

Kim¹,

Seo²,

Yasunaga³

et al. 2006

2006 International Conference on Hybrid Information Technology

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In current bioinformatics, the prediction of transcriptional regulatory elements (TREs) is one of the major challenges. It is a key step toward deciphering entire genetic regulatory networks. The goal of this study is to predict TREs associated with four classes of developmentally regulated genes in Dictyostelium discoideum (Dd). Applying a pattern recognition method to 5' untranscribed region of Dd sequences, the evaluation score (E-score) for each candidate TRE is calculated. Based on these E-scores and the proposed selection criteria, putative class-specific TREs are predicted in each developmentally regulated class of Dd.

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Identification of novel elements which regulate the cell-type specificity of Dictyostelium 7E gene expression

Cited by 6 publications

References 47 publications

TheDictyosteliumbZIP transcription factor DimB regulates prestalk-specific gene expression

TheDictyosteliumbZIP transcription factor DimB regulates prestalk-specific gene expression

Regulation of ecmF gene expression and genetic hierarchy among STATa, CudA, and MybC on several prestalk A‐specific gene expressions in Dictyostelium

A New Approach to Predicting Regulatory Elements in Dictyostelium Gene Expression

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