Identification of novel diagnostic and prognostic miRNA signatures in endometrial cancer

Abstract

With the goal of identifying diagnostic and prognostic biomarkers in endometrial cancer, miRNA-profiling was carried out with formalin-fixed paraffin embedded (FFPE) tissue samples from 49 endometrial cancer patients. Results using an 84-cancer specific miRNA panel identified the upregulation of miR-141-3p and miR-96-5p along with a downregulation of miR-26, miR-126-3p, miR-23b, miR-195-5p, miR-374a and let-7 family of miRNAs in endometrial cancer. We validated the dysregulated expression of the identified miRNAs in a panel of endometrial cancer cell-lines. Immunohistochemical analysis of the tissue micro array derived from these patients established the functional correlation between the decreased expression of tumor suppressive miRNAs and their target oncogenes: ERBB2, EGFR, EPHA2, BAX, GNA12, GNA13, and JUN. Comparative analysis of the samples from the patients with extended progression-free survival (PFS) ( > 21 months) versus the patients with the PFS of < 21 months indicated increased expression of tumor suppressive miR-142-3p, miR-142-5p, and miR-15a-5p in samples from extended PFS patients. In addition to defining a specific set of miRNAs and their target genes as potential diagnostic biomarkers, our studies have identified tumor suppressive miR-142 cluster and miR-15a as predictors of favorable prognosis for therapy response in endometrial cancer.

Integrated Analysis Reveals That miR-193b, miR-671, and TREM-1 Correlate With a Good Response to Treatment of Human Localized Cutaneous Leishmaniasis Caused by Leishmania braziliensis

“…These results were validated in serum samples and showed higher specificity and sensibility then PSA (Prostate-specific antigen) testing ( 112 ). Similar results were also obtained for different types of cancer, where circulating miRNAs are potential markers of disease progression and response to treatment, as well as a promising screening test for cancer detection ( 12 , 116 – 118 ). However, there is a lack of studies suggesting the potential of miRNAs as markers of infectious/parasitic diseases in humans.…”

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

“…There was less consensus on miRNAs that are downregulated in women with endometrial cancer (Table 2), with 44 different miRNAs being down-regulated. However, all miRNAs were only reported in 1 article [15,21,30,32,[34][35][36][37][38][39]. MiR-137 and miR-129-3p showed the largest mean fold change (115.15 and 42.30 respectively) but were only found in a small cohort (sample size EC 23 and sample size control 4 patients) [33].…”

Non-Coding RNAs and Endometrial Cancer

“…Furthermore, increased levels of miR-944 were observed in EC cell lines. miR-944 expression was associated with the EC stages and pathology results of the International Federation of Gynecology and Obstetrics (FIGO) [ 64 ].…”