Identification of novel cyclic lipopeptides from a positional scanning combinatorial library with enhanced antibacterial and antibiofilm activities

Bionda, Nina; Fleeman, Renee; Fuente-Núñez, César de la; Rodrı́guez, Marı́a C.; Reffuveille, Fany; Shaw, Lindsey N.; Pastar, Irena; Davis, Stephen C.; Hancock, Robert E. W.; Čudić, Predrag

doi:10.1016/j.ejmech.2015.11.032

Cited by 50 publications

(30 citation statements)

References 62 publications

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“…The sample was combined with an additional 4 mL of neutralizing solution and homogenized. Serial dilutions were made from all culture samples and the extent of microbiological contamination assessed using the Spiral Plater System (Spiral Biotech, Norwood, MA) as previously described . Pseudomonas Agar‐base supplemented with Cetrimide Sodium Nalidixate (Remel Inc, Lenexa, KS) was used to isolate P. aeruginosa from the wound biofilms.…”

Section: Methodsmentioning

confidence: 99%

Preclinical evaluation of a novel silver gelling fiber dressing on Pseudomonas aeruginosa in a porcine wound infection model

Davis

Gil

et al. 2019

Wound Repair Regeneration

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The wound environment is a fertile ground for biofilm forming pathogens. Once biofilms form within the wound, they can be very challenging to eradicate. The purpose of this study was to examine the effect of a gelling fiber dressing with silver using a well‐established porcine wound biofilm model. Deep partial thickness wounds were inoculated with Pseudomonas aeruginosa ATCC 27312 and covered with a polyurethane film dressing to promote biofilm formation. Wounds were then divided into treatment groups: gelling fiber dressing with silver, gelling fiber dressing without silver, hydrofiber dressing with silver, benzethonium chloride and ethylenediaminetetraacetic acid and compared to untreated control. Microbiological, biofilm, and histological wound assessments were performed on days 3, 5, and 7 postinfection. Treatment with gelling fiber dressing with silver resulted in significant reduction of P. aeruginosa biofilm when compared to all other treatment groups on every assessment time point. In addition, gelling fiber dressing with silver treatment resulted in detachment of biofilm from the wound, while wounds treated with gelling fiber dressing with and without silver showed more granulation tissue formation on day 3. Our data show that a new gelling fiber dressing with silver was effective in reducing biofilm associated P. aeruginosa in vivo. This study may have important clinical implications especially for wounds heavily colonized with gram‐negative biofilm‐forming bacteria.

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Section: Methodsmentioning

confidence: 99%

Preclinical evaluation of a novel silver gelling fiber dressing on Pseudomonas aeruginosa in a porcine wound infection model

Davis

Gil

et al. 2019

Wound Repair Regeneration

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“…Secondly, Bionda et al . [15] used a positional-scanning combinatorial method to screen a cyclic lipopeptide library (peptides derived from fusaricidin/LI-F) against multidrug resistant pathogens. The lead peptide from this study showed activity against all ESKAPE pathogens at 110 μM.…”

Section: Antimicrobial Peptides With Potential To Fight Biofilm-relatmentioning

confidence: 99%

Anti-biofilm peptides as a new weapon in antimicrobial warfare

Pletzer

Coleman

Hancock

2016

Current Opinion in Microbiology

146

107

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Microorganisms growing in a biofilm state are very resilient in the face of treatment by many antimicrobial agents. Biofilm infections are a significant problem in chronic and long-term infections, including those colonizing medical devices and implants. Anti-biofilm peptides represent a very promising approach to treat biofilm-related infections and have an extraordinary ability to interfere with various stages of the biofilm growth mode. Anti-biofilm peptides possess promising broad-spectrum activity in killing both Gram-positive and Gram-negative bacteria in biofilms, show strong synergy with conventional antibiotics, and act by targeting a universal stringent stress response. Understanding downstream processes at the molecular level will help to develop and design peptides with increased activity. Anti-biofilm peptides represent a novel, exciting approach to treating recalcitrant bacterial infections.

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“…When wounds become colonized with biofilm-forming bacteria, the ability to heal is compromised and they can become very difficult to treat 13. Leg ulcers, a common chronic wound often found in immunocompromised patients,14 are predominantly colonized by P. aeruginosa (88%) and Staphylococcus aureus (33%),3 two of the most prevalent species found in chronic wound biofilms 15,16. Furthermore, resistance to the antibiotics routinely used to treat biofilms only augments the crisis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

Draughn¹,

Allen²,

Routh

et al. 2017

DDDT

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2-Aminoimidazole (2-AI)-based compounds have been shown to efficiently disrupt biofilm formation, disperse existing biofilms, and resensitize numerous multidrug-resistant bacteria to antibiotics. Using Pseudomonas aeruginosa and Staphylococcus aureus, we provide initial pharmacological studies regarding the application of a 2-AI as a topical adjuvant for persistent dermal infections. In vitro assays indicated that the 2-AI H10 is nonbactericidal, resensitizes bacteria to antibiotics, does not harm the integument, and promotes wound healing. Furthermore, in vivo application of H10 on swine skin caused no gross abnormalities or immune reactions. Taken together, these results indicate that H10 represents a promising lead dermal adjuvant compound.

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Identification of novel cyclic lipopeptides from a positional scanning combinatorial library with enhanced antibacterial and antibiofilm activities

Cited by 50 publications

References 62 publications

Preclinical evaluation of a novel silver gelling fiber dressing on Pseudomonas aeruginosa in a porcine wound infection model

Preclinical evaluation of a novel silver gelling fiber dressing on Pseudomonas aeruginosa in a porcine wound infection model

Anti-biofilm peptides as a new weapon in antimicrobial warfare

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

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