Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer

Masuda, Tsuneyuki; Nagai, Makoto; Yamasato, Hiroshi; Tsuchiaka, Shinobu; Okazaki, Sachiko; Katayama, Yukie; Oba, Mami; Nishiura, Naomi; Sassa, Yukiko; Omatsu, Tsutomu; Furuya, Tetsuya; Koyama, Satoshi; Shirai, Junsuke; Taniguchi, Koki; Fujii, Yoshiki; Todaka, Reiko; Katayama, Kazuhiko; Mizutani, Tetsuya

doi:10.1016/j.vetmic.2014.03.009

Cited by 39 publications

(40 citation statements)

References 44 publications

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“…Reassortment events between humans and animal strains have been found previously (Banyai et al., ; Doan et al., ; Esona et al., , ; Ghosh et al., ; He et al., , ; Jere, Mlera, O'Neill, Peenze, & van Dijk, ; Malik et al., ; Masuda et al., ; Matthijnssens et al., ; Mullick et al., ; Steyer, Sagadin, Kolenc, & Poljsak‐Prijatelj, ). Of the 11 segments of the RVA/Pig/China/LNCY/2016/G3P[13] strain, nine gene segments (VP1, VP2, VP3, VP4, VP6, NSP1, NSP2, NSP3 and NPS5) possessed sequences nearly identical with those of the porcine strains, suggesting that RVA/Pig/China/LNCY/2016/G3P[13] was possibly of porcine origin.…”

Section: Discussionmentioning

confidence: 58%

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

Jing

Zhang

Shi

et al. 2017

Transbound Emerg Dis

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Group A rotaviruses (RVAs) are a major cause of serious intestinal disease in piglets. In this study, a novel pig strain was identified in a stool sample from China. The strain was designated RVA/Pig/China/LNCY/2016/G3P[13] and had a G3-P[13]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genome. The viral protein 7 (VP7) and non-structural protein 4 (NSP4) genes of RVA/Pig/China/LNCY/2016/G3P[13] were closely related to cogent genes of human RVAs, suggesting that a reassortment between pig and human strains had occurred. Recombination analysis showed that RVA/Pig/China/LNCY/2016/G3P[13] is a natural recombinant strain between the P[23] and P[7] RVA strains, and crossover points for recombination were found at nucleotides (nt) 456 and 804 of the VP4 gene. Elucidating the biological characteristics of porcine rotavirus (PoRV) will be helpful for further analyses of the epidemic characteristics of this virus. The results of this study provide valuable information for RVA recombination and evolution and will facilitate future investigations into the molecular pathogenesis of RVAs.

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Section: Discussionmentioning

confidence: 58%

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

Jing

Zhang

Shi

et al. 2017

Transbound Emerg Dis

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“…Neither cytopathic effect nor the expected RT-PCR products from RNA extracted from cell culture supernatants were observed in any of the inoculated cells, indicating a lack of virus replication in these cell lines. However, using RNA-seq based strategy with DNase treatment before construction of cDNA libraries, we could obtain sufficient viral genomic sequences of RNA viruses from fecal samples not only for picornaviruses but also, in earlier studies, for other viruses such as rotavirus (Minami-Fukuda et al, 2013;Masuda et al, 2014;Nagai et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Identification and complete genome analysis of a novel bovine picornavirus in Japan

et al. 2015

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We identified novel viruses in feces from cattle with diarrhea collected in 2009 in Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the (near) complete sequences of the virus. Sequence analyses revealed that they had a standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1 (VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group 1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9% (P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses and detailed genome characterization showed that they, together with the unclassified Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding regions. These viruses had conserved features (e.g. predicted protein cleavage sites, presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they have a common ancestor. Reverse-transcription-PCR assays, using specific primers designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal samples from cattle with diarrhea were positive, indicating the prevalence of these picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further studies are needed to investigate the pathogenic potential and etiological role of these viruses in cattle.

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“…The VP4 gene of RVA/Human-wt/GTM/2009726790/2009/G8 P[14] strain was 97.8% identical to a bovine G15P[14] strain (Tottori) (Masuda et al, 2014), 86–96.1% identical to bovine G8P[14] strains and 83.7–87.5% identical to human G8P[14] strains. Strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] VP4 gene occupies lineage VII shared with Tottori-G15P[14] and Sun9-G8P[14] strains from Japan (Fig.…”

mentioning

confidence: 99%

Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala

Gautam

Mijatovic-Rustempasic

Roy

et al. 2015

Infection, Genetics and Evolution

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We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. This strain was designated as RV A/Human-wt/GTM/2009726790/2009/G8P[14], with a genomic constellation of G8-P[14]-I2-R2-C2-M2-A13-N2-T6-E2-H3. The VP4 gene occupied lineage VII within the P[14] genotype. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event. The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.

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Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer

Cited by 39 publications

References 44 publications

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

Identification and complete genome analysis of a novel bovine picornavirus in Japan

Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala

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