Identification of novel alternative splicing of bovine lncRNA lncFAM200B and its effects on preadipocyte proliferation

Abstract

Adipogenesis is closely related to human health, livestock growth, and meat quality. A previous study identified that bovine lncFAM200B promoter has high activity in 3T3-L1 mice preadipocytes. Thus, lncFAM200B was a candidate gene for regulating adipogenesis. This study aimed to uncover the role of lncFAM200B in bovine adipogenesis and identify novel genetic variations within the bovine lncFAM200B gene. An expression analysis found that lncFAM200B was expressed higher in fat than that in muscle, but the difference was not related to the total methylation level of the promoter active region. Moreover, the expression of lncFAM200B exhibited a significant positive correlation with the expression of C/EBPa during bovine adipocyte differentiation. To uncover the function of lncFAM200B, the full-length lncFAM200B was cloned, and four kinds of transcript variants were found. Protein-coding potential prediction and prokaryotic expression system analysis showed that these four transcript variants were noncoding RNAs. The quantitative reversetranscription polymerase chain reaction and 5-ethynyl-2′-deoxyuridine assay showed that the transcript variants decreased the messenger RNA expression of Cyclin D1 and inhibited the proliferation of bovine preadipocytes. Considering the important role of lncFAM200B in adipogenesis, we identified genetic variations in lncFAM200B. Three single-nucleotide polymorphisms (SNPs) were revealed, and two of them (SNP1 and SNP3) were associated with Nanyang cattle body measurement traits. In conclusion, this study found that bovine lncFAM200B inhibited preadipocyte proliferation, and two genetic variations of lncFAM200B could be used in cattle breeding.

Regulatory Network of miRNA, lncRNA, Transcription Factor and Target Immune Response Genes in Bovine Mastitis

“…Furthermore, we identi ed important interactions between certain lncRNAs and mRNAs that could mediate host immune response at the post-transcriptional level. lncRNAs are becoming known to be involved in several biogenetic processes that may regulate disease pathogenesis 14,44 . We hypothesized that lncRNAs that bind to multiple mRNA target sequences could serve as drug targets and have the potential to modify or regulate immune signaling pathways, ameliorating the over-or under-production of cytokines or chemokine during infection.…”

circFLT1 and lncCCPG1 Sponges miR-93 to Regulate the Proliferation and Differentiation of Adipocytes by Promoting lncSLC30A9 Expression

“…Previous studies have underlined the significance of ncRNAs in adipogenesis, especially in regulating the proliferation and differentiation of adipocytes. 10 , 11 , 12 , 13 The expression of ncRNAs is specific to cells and tissues and may differ between various stages of differentiation. This observation suggests that ncRNAs may be fine-tuning factors of cell fate.…”

The role of long noncoding RNAs in livestock adipose tissue deposition

“…In livestock production, a novel lncRNA mainly localized to the nucleus and named miR-221 host gene (MIR221HG) because it overlaps with miR-221 in the genome was proven to regulate bovine adipocyte differentiation [69]. Studies found that the expression of lnc FAM200B in bovines had a significant positive correlation with C/EBPα expression, suggesting that lnc FAM200B might participate in the regulation of adipocyte development [70]. In addition, evidence indicated that PSXV-9, a novel antisense lncRNA of C/EBPα, could inhibit bovine adipogenic differentiation [71].…”