circFLT1 and lncCCPG1 Sponges miR-93 to Regulate the Proliferation and Differentiation of Adipocytes by Promoting lncSLC30A9 Expression

Kang, Zihong; Zhang, Sihuang; Jiang, Enhui; Wang, Xinyu; Wang, Zhen; Chen, Hong; Lan, Xianyong

doi:10.1016/j.omtn.2020.09.011

Cited by 28 publications

(22 citation statements)

References 27 publications

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“…have identified that six circRNAs play potential functions during yak adipocyte differentiation. Kang et al [ 88 ] have discovered that circFLT1 and lncCCPG1 are differentially expressed in bovine adipocytes. Overexpression of circFLT1 and lncCCPG1 together promotes adipocyte differentiation and inhibits adipocyte proliferation.…”

Section: Circrnas Related To Adipocyte Metabolismmentioning

confidence: 99%

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Zhang

Tian

et al. 2022

Cell Death Discov.

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As noncoding RNAs, circular RNAs (circRNAs) are covalently enclosed endogenous biomolecules in eukaryotes that have tissue specificity and cell specificity. circRNAs were once considered a rare splicing byproduct. With the development of high-throughput sequencing, it has been confirmed that they are expressed in thousands of mammalian genes. To date, only a few circRNA functions and regulatory mechanisms have been verified. Adipose is the main tissue for body energy storage and energy supply. Adipocyte metabolism is a physiological process involving a series of genes and affects biological activities in the body, such as energy metabolism, immunity, and signal transmission. When adipocyte formation is dysregulated, it will cause a series of diseases, such as atherosclerosis, obesity, fatty liver, and diabetes. In recent years, many noncoding RNAs involved in adipocyte metabolism have been revealed. This review provides a comprehensive overview of the basic structure and biosynthetic mechanism of circRNAs, and further discusses the circRNAs related to adipocyte formation in adipose tissue and liver. Our review will provide a reference for further elucidating the genetic regulation mechanism of circRNAs involved in adipocyte metabolism.

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Section: Circrnas Related To Adipocyte Metabolismmentioning

confidence: 99%

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Zhang

Tian

et al. 2022

Cell Death Discov.

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“…The 179/192-bp DNA sequence containing the 13-bp region was cloned into psiCHECK-2 using T4 DNA Ligase and the insert was released by XhoI and NotI digestion (TaKaRa Biotech Co., Ltd.). The miR-181 sequence was cloned into the pcDNA3.1 vector (Invitrogen) as described previously (Kang et al, 2020;Hao et al, 2021), and the insert was released by KpnI and XhoI digestion.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Goat AKAP12: Indel Mutation Detection, Association Analysis With Litter Size and Alternative Splicing Variant Expression

et al. 2021

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A-kinase anchoring protein 12 (AKAP12) plays key roles in male germ cells and female ovarian granulosa cells, whereas its influence on livestock litter size remains unclear. Herein we detected the genetic variants of AKAP12 gene and their effects on litter size as well as alternative splicing variants expression in Shaanbei white cashmere (SBWC) goats, aiming at exploring theoretical basis for goat molecular breeding. We identified two Insertion/deletions (Indels) (7- and 13-bp) within the AKAP12 gene. Statistical analyses demonstrated that the 13-bp indel mutation in the 3′ UTR was significantly associated with litter size (n = 1,019), and the carriers with DD genotypes presented lower litter sizes compared with other carriers (P < 0.01). Bioinformatics analysis predicted that this 13-bp deletion sequence could bind to the seed region of miR-181, which has been documented to suppress porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163 and affect the pig litter size. Therefore, luciferase assay for this 13-bp indel binding with miRNA-181 was performed, and the luciferase activity of pcDNA-miR-181-13bp-Deletion-allele vector was significantly lower than that of the pcDNA-miR-181-13bp-Insertion-allele vector (P < 0.05), suggesting the reduced binding capability with miR-181 in DD genotype. Given that alternative spliced variants and their expression considerably account for the Indel genetic effects on phenotypic traits, we therefore detected the expression of the alternative spliced variants in different tissues and identified that AKAP12-AS2 exhibited the highest expression levels in testis tissues. Interestingly, the AKAP12-AS2 expression levels of homozygote DD carriers were significantly lower than that of individuals with heterozygote ID, in both testis and ovarian tissues (P < 0.05), which is consistent with the effect of the 13-bp deletion on the reduced litter size. Taken together, our results here suggest that this 13-bp indel mutation within goat AKAP12 might be utilized as a novel molecular marker for improving litter size in goat breeding.

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“…CircINSR could target miR-15/16 to inhibit the pre-mature differentiation of intramuscular preadipocytes [ 15 ]. CircFLT1 and lncCCPG1 sponges miR-93 to regulate the proliferation and differentiation of adipocytes in bovine [ 16 ]. These discoveries demonstrate the importance of lncRNAs and circRNAs in adipogenesis.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Differentially Expressed mRNAs, lncRNAs and circRNAs Related to Intramuscular Fat Deposition in Laiwu Pigs

Zhao

Wang

et al. 2022

Genes

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Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) are important classes of small noncoding RNAs that can regulate numerous biological processes. To understand the role of message RNA (mRNAs, lncRNAs and circRNAs) in the regulation of intramuscular fat (IMF) deposition, in this study the expression profiles of longissimus dorsi (LD) muscle from six Laiwu pigs (three with extremely high and three with extremely low IMF content) were sequenced based on rRNA-depleted library construction. In total, 323 differentially expressed protein-coding genes (DEGs), 180 lncRNAs (DELs) and 105 circRNAs (DECs) were detected between the high IMF and low IMF groups. Functional analysis indicated that most DEGs, and some target genes of DELs, were enriched into GO terms and pathways related to adipogenesis, suggesting their important roles in regulating IMF deposition. In addition, 12 DELs were observed to exhibit a positive relationship with stearoyl-CoA desaturase (SCD), phosphoenolpyruvate carboxykinase 1(PCK1), and adiponectin (ADIPOQ), suggesting they are highly likely to be the target genes of DELs. Finally, we constructed a source gene-circRNA-miRNA connective network, and some of miRNA of the network have been reported to affect lipid metabolism or adipogenesis. Overall, this work provides a valuable resource for further research and helps to understand the potential functions of lncRNAs and circRNAs in IMF deposition.

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circFLT1 and lncCCPG1 Sponges miR-93 to Regulate the Proliferation and Differentiation of Adipocytes by Promoting lncSLC30A9 Expression

Cited by 28 publications

References 27 publications

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Goat AKAP12: Indel Mutation Detection, Association Analysis With Litter Size and Alternative Splicing Variant Expression

Comprehensive Analysis of Differentially Expressed mRNAs, lncRNAs and circRNAs Related to Intramuscular Fat Deposition in Laiwu Pigs

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