Identification of nicotianamine synthase genes in Triticum monococcum and their expression under different Fe and Zn concentrations

Du, Xuye; Wang, Huinan; He, Jiefang; Zhu, Bin; Guo, Juan; Hou, Wenqian; Weng, Qingbei; Zhang, Xiaocun

doi:10.1016/j.gene.2018.06.015

Cited by 11 publications

(11 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that adding exogenous PUT to the nutrient solution triggers NO accumulation under both Fe-sufficient and Fe-deficient conditions (Zhu et al 2016). The synthesis of polyamines occurs from S-adenosyl methionine (Dandan et al 2019), which is also a precursor of nicotianamine (NA), a strong natural Fe-chelator (Diaz-Benito et al 2018;Du et al 2018). Nicotianamine plays a pivotal role in cellular Fe transport, and is needed for the intra-and inter-cellular distribution of Fe and other metals in young leaf tissues, reproductive organs, and seeds (Takahashi et al 2003;Chen et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Foliar applications of thidiazuron and putrescine increase leaf iron and chlorophyll concentrations in iron-deficient pot marigold (Calendula officinalis L.)

2021

View full text Add to dashboard Cite

The study was aimed to determine the effects of foliar applications of thidiazuron and putrescine, two compounds that may cause iron (Fe) remobilization, in pot marigold (Calendula officinalis L.) grown in controlled conditions under a limited Fe supply. In a first experiment, plants were grown in a greenhouse in a mixture of sand and perlite with pre-growth/growth Fe concentrations ranging from 0 to 20 µM, and treated three consecutive times with foliar sprays of 0 or 45.4 µM thidiazuron. In a second experiment, plants were grown in a greenhouse in hydroponics with pre-growth/growth Fe concentrations in the nutrient solution ranging from 0 to 20 µM, and treated three consecutive times with foliar sprays of 0, 2.27, or 5.67 mM putrescine. Parameters measured included leaf photosynthetic pigments and Fe concentrations, root ferric chelate reductase activities, photosynthesis rates and peroxidase in leaf extracts in the first experiment, and leaf photosynthetic pigments, leaf and root micronutrient concentrations, root ferric chelate reductase activities, and superoxide dismutase and peroxidase activities in leaf extracts in the second experiment. Results indicate that foliar thidiazuron and putrescine treatments in the µM and mM ranges, respectively, improve Fe transport to the leaf under zero or low supply of Fe. This indicates that foliar treatments with thidiazuron and putrescine increase remobilization of pre-existing plant Fe pools. This could be an additional tool for the optimization of Fe nutrition in ornamental plants such as C. officinalis when grown in controlled conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Foliar applications of thidiazuron and putrescine increase leaf iron and chlorophyll concentrations in iron-deficient pot marigold (Calendula officinalis L.)

2021

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…NAS genes are encoded by multi-gene families in a broad range of graminaceous plants, including maize, rice, wheat, and barley [ 32 , 42 – 44 ]. NAS genes are grouped into class I and class II [ 32 , 44 ]. Class I NAS genes are preferentially expressed in root and stem tissues, and their expression is up-regulated in response to iron deficiency.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of the NAAT, DMAS, TOM, and ENA gene families in maize suggests their roles in mediating iron homeostasis

Zhang

Xiao

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Nicotianamine (NA), 2′-deoxymugineic acid (DMA), and mugineic acid (MA) are chelators required for iron uptake and transport in plants. Nicotianamine aminotransferase (NAAT), 2′-deoxymugineic acid synthase (DMAS), transporter of MAs (TOM), and efflux transporter of NA (ENA) are involved in iron uptake and transport in rice (Oryza sativa), wheat (Triticum aestivum), and barley (Hordeum vulgare); however, these families have not been fully identified and comprehensively analyzed in maize (Zea mays L.). Results Here, we identified 5 ZmNAAT, 9 ZmDMAS, 11 ZmTOM, and 2 ZmENA genes by genome mining. RNA-sequencing and quantitative real-time PCR analysis revealed that these genes are expressed in various tissues and respond differently to high and low iron conditions. In particular, iron deficiency stimulated the expression of ZmDMAS1, ZmTOM1, ZmTOM3, and ZmENA1. Furthermore, we determined protein subcellular localization by transient expression of green fluorescent protein fusions in maize mesophyll protoplasts. ZmNAAT1, ZmNAAT-L4, ZmDMAS1, and ZmDMAS-L1 localized in the cytoplasm, whereas ZmTOMs and ZmENAs targeted to plasma and tonoplast membranes, endomembranes, and vesicles. Conclusions Our results suggest that the different gene expression profiles and subcellular localizations of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA family members may enable specific regulation of phytosiderophore metabolism in different tissues and under different external conditions, shedding light on iron homeostasis in maize and providing candidate genes for breeding iron-rich maize varieties.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Many studies revealed that NAS genes are encoded by multi-gene families in a broad range of graminaceous plants, including maize, rice, wheat and barley [15,[41][42][43]. ZmNAS genes were rstly grouped into two classes, class I and class II, and then this classi cation was further applied in other species [41,43]. Moreover, it was found that class I NAS genes were preferentially expression in root and stem, and their expressions were up-regulated in response to iron de ciency; while class II genes were mainly expressed in leaf and they were induced under excess iron conditions [41,44,45].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of NAAT, DMAS, TOM, and ENA gene families in maize reveals their roles in regulating iron homeostasis

Zhang¹,

Zhou²,

Li³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Nicotianamine (NA) serves as not only the major chelator for iron transport but also the intermediate for synthesizing mugineic acid family phytosiderophores (MAs) which are secreted by graminaceous plants for Fe uptake. Therefore, the production and secretion of MAs are key steps for maintaining iron homeostasis in plants. Nicotianamine aminotransferase (NAAT), 2’-deoxymugineic acid synthase (DMAS), MAs efflux transporter (TOM), and efflux transporter of NA (ENA) were identified to be involved in these processes in rice and barley, whereas little systematic study has been performed in maize (Zea mays.L). Results: Here, we identified five ZmNAAT, nine ZmDMAS, eleven ZmTOM, and two ZmENA genes in maize by genome mining. RNA-sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of these genes exhibited diverse tissue specificity and different responses to environmental iron conditions. Moreover, the expression patterns were related to their evolution relationships. In particular, the ZmNAAT family can be classified into two subgroups, with one group showed inhibited expression in root under iron excess status and another subclass were repressed in shoot under both iron deficiency and excess. Likewise, the expression of ZmDMAS1 was stimulated under iron deficiency, while the remaining genes fell into two sub-clades with different expression patterns. Significant up-regulation of ZmTOM1, ZmTOM3 and ZmENA1 were observed under iron starvation, while ZmTOM2 was induced under both iron-excess and deficiency. These results reflect changing demands for the synthesis and secretion of NA/MAs to balance iron homeostasis under fluctuating conditions. All the examined ZmNAAT and ZmDMAS proteins localized in cytoplasm, while plasma and tonoplast membrane, endomembrane, and vesicle localization were observed for ZmTOM and ZmENA proteins. These results indicate that ZmTOM and ZmENA proteins may contribute to not only intercellular export but also intracellular sequestration of NA and MAs to facilitate iron homeostasis. Conclusions: Our results suggest that different gene expression profiles and subcellular localization of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA members may enable dedicate regulation of NA and phytosiderophores (PS) metabolism, shedding light on the understanding of iron-homeostasis in maize. Additionally, we also provided candidate genes for breeding iron-rich maize varieties.

show abstract

Identification of nicotianamine synthase genes in Triticum monococcum and their expression under different Fe and Zn concentrations

Cited by 11 publications

References 32 publications

Foliar applications of thidiazuron and putrescine increase leaf iron and chlorophyll concentrations in iron-deficient pot marigold (Calendula officinalis L.)

Foliar applications of thidiazuron and putrescine increase leaf iron and chlorophyll concentrations in iron-deficient pot marigold (Calendula officinalis L.)

Genome-wide analysis of the NAAT, DMAS, TOM, and ENA gene families in maize suggests their roles in mediating iron homeostasis

Genome-wide analysis of NAAT, DMAS, TOM, and ENA gene families in maize reveals their roles in regulating iron homeostasis

Contact Info

Product

Resources

About