Identification of new small molecules as dual FoxM1 and Hsp70 inhibitors using computational methods

Abstract

Background and purpose: FoxM1 and Hsp70 proteins are highly expressed in many cancers. Thus, their inhibition serves as Bonafede targets in cancer treatment. Experimental approach: FDI-6, an inhibitor of FoxM1, was selected as a template, and based on its structure, a new library from the ZINC database was obtained. Virtual screening was then performed using the created pharmacophore model. The second virtual screening phase was conducted with molecular docking to get the best inhibitor for both FoxM1 and Hsp70 active sites. In silico , ADMET properties were also calculated. Finally, molecular dynamics simulation was performed on the best ligand, ZINC1152745, for both Hsp70 and FoxM1 proteins during 100 ns. Findings / Results: The results of this study indicated that ZINC1152745 was stable in the active site of both proteins, Hsp70 and FoxM1. The final scaffold identified by the presented computational approach could offer a hit compound for designing promising anticancer agents targeting both FoxM1 and Hsp70. Conclusion and implications: Molecular dynamics simulations were performed on ZINC1152745 targeting FoxM1 and Hsp70 active sites. The results of several hydrogen bonds, the radius of gyration, RMSF, RMSD, and free energy during the simulations showed good stability of ZINC1152745 with FoxM1 and Hsp70.

Evaluation of in vitro and in vivo anticancer activities of potassium koetjapate: a solubility improved formulation of koetjapic acid against human colon cancer

“…Interestingly, it was found that down-regulation of HSP60 provoked by IGFBP7 may trigger IGFBP7's tumor-suppressive natural behavior in CRC ( 23 ). It was also reported that HSP70 plays a significant role in the suppression of the apoptosis pathway ( 24 25 ). The findings of the current study highlighted that KKA significantly up-regulated the expression of IGFBP-2 and IGFBP-6 while down-regulating the expression of IGF-1, HSP60 as well as HSP70 proteins.…”