Identification of Nematicidal Constituents of Notopterygium incisum Rhizomes against Bursaphelenchus xylophilus and Meloidogyne incognita

Abstract

During a screening program for new agrochemicals from Chinese medicinal herbs, the ethanol extract of Notopterygium incisum rhizomes was found to possess strong nematicidal activity against the two species of nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. Based on bioactivity-guided fractionation, the four constituents were isolated from the ethanol extract and identified as columbianetin, falcarindiol, falcarinol, and isoimperatorin. Among the four isolated constituents, two acetylenic compounds, falcarindiol and falcarinol (2.20-12.60 µg/mL and 1.06-4.96 µg/mL, respectively) exhibited stronger nematicidal activity than two furanocoumarins, columbianetin, and isoimperatorin (21.83-103.44 µg/mL and 17.21-30.91 µg/mL, respectively) against the two species of nematodes, B. xylophilus and M. incognita. The four isolated constituents also displayed phototoxic activity against the nematodes. The results indicate that the ethanol extract of N. incisum and its four isolated constituents have potential for development into natural nematicides for control of plant-parasitic nematodes.

Epigenetic Manipulation Induces the Production of Coumarin‐Type Secondary Metabolite from Arthrobotrys foliicola

“…Plant‐parasitic nematodes have caused severe damage to agriculture and forestry worldwide over the past few decades. It has been considered that plant‐parasitic nematodes cause more than $100 billion worth of losses to the global agriculture and trees annually ,,. Currently, the main strategy for controlling nematodes is based upon using the synthetic chemical agents combined with agriculture practice for the nematode management ,.…”

“…Moreover, no coumarin‐like secondary metabolite has been isolated either from Arthrobotrys genus or from Orbiliaceae family which implies the importance of epigenetic modulation in triggering the production of coumarins (compound 1 in our study). These compounds were previously reported for their nematocidal activity against phytonematode ,,. Furthermore, several coumarin‐related compounds displayed excellent nematocidal activity and were investigated for the further development of efficient nematocidal agents…”

“…It has been considered that plant-parasitic nematodes cause more than $100 billion worth of losses to the global agriculture and trees annually. [34,35,36] Currently, the main strategy for controlling nematodes is based upon using the synthetic chemical agents combined with agriculture practice for the nematode management. [36,37] However, the chemical products have generated several problems such as the resistance to chemical nematicides, high risks to human health, and the environment pollution.…”

“…[34,35,36,38,39] In order to discover more effective and environmental friendly nematode management, natural products or their derivatives have been investigated as the alternatives to synthetic chemical nematicidal agents. [34,36,37,38,40] Fungi of Arthrobotrys genus are well-known for their nematode-trapping ability and nematocidal activity. Previously, some of oligosporon group antibiotics isolated from Arthrobotrys species were reported to exert moderate nematocidal activity.…”

“…[36,37] However, the chemical products have generated several problems such as the resistance to chemical nematicides, high risks to human health, and the environment pollution. [34,35,36,38,39] In order to discover more effective and environmental friendly nematode management, natural products or their derivatives have been investigated as the alternatives to synthetic chemical nematicidal agents. [34,36,37,38,40] Fungi of Arthrobotrys genus are well-known for their nematode-trapping ability and nematocidal activity.…”