Identification of naphthalene metabolism by white rot fungus Pleurotus eryngii

Abstract

The use of biomaterials or microorganisms in PAHs degradation had presented an eye-catching performance. Pleurotus eryngii is a white rot fungus, which is easily isolated from the decayed woods in the tropical rain forest, used to determine the capability to utilize naphthalene, a two-ring polycyclic aromatic hydrocarbon as source of carbon and energy. In the meantime, biotransformation of naphthalene to intermediates and other by-products during degradation was investigated in this study. Pleurotus eryngii had been incubated in liquid medium formulated with naphthalene for 14 days. The presence of metabolites of naphthalene suggests that Pleurotus eryngii begin the ring cleavage by dioxygenation on C1 and C4 position to give 1,4-naphthaquinone. 1,4-Naphthaquinone was further degraded to benzoic acid, where the proposed terepthalic acid is absent in the cultured extract. Further degradation of benzoic acid by Pleurotus eryngii shows the existence of catechol as a result of the combination of decarboxylation and hydroxylation process. Unfortunately, phthalic acid was not detected in this study. Several enzymes, including manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase are enzymes responsible for naphthalene degradation. Reduction of naphthalene and the presence of metabolites in liquid medium showed the ability of Pleurotus eryngii to utilize naphthalene as carbon source instead of a limited glucose amount.

Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032

“…The fungus Pleurotus eryngii F032 has been extensively investigated for its application in the degradation of naphthalene, a two-ring aromatic compound (Hadibarata et al 2013). Laccase has the powerful mechanism to degrade phenolic and non-phenolic compounds, while the secretion of lignin peroxidase (LiP) by fungi will enhance the decolorization of dye (Hadibarata et al 2012a, b;Hazeroual et al 2006).…”

Transcriptome Sequencing and Comparative Analysis of Piptoporus betulinus in Response to Birch Sawdust Induction

“…Published findings have evaluated the effect of white-rot fungi on removal of gaseous chlorobenzene and naphthalene, while no reports have been published regarding this degradation using brown-rot fungi. Therefore, our next experiment would determine the ability of gaseous chlorobenzene or naphthalene in P. betulinus [36][37][38]. The findings of Krueger et al [38] indicated that brown-rot fungus Gloeophyllum trabeum might be suitable for the biodegradation of styrene.…”

Biosorption and biotransformation of fluoranthene by the white‐rot fungus Pleurotus eryngii F032

“…The strain P. eryngii F032 was isolated from decayed woods in Indonesia's tropical rain forest. Isolation, identification, and culture media are described elsewhere .…”

“…Batch growth experiments of P. eryngii F032 were performed in 100‐mL conical flasks containing 20 mL of modified mineral salt broth medium (MSB) supplemented with three agar plugs (5 mm in diameter) and a substrate solution . The substrate solution consisted of fluoranthene (5 mg/L), dimethylformamide, and chloramphenicol to prevent the growth of bacteria.…”

“…Although biodegradation appears to be an effective method for treating water contaminated with PAHs, the main biosorption process and capacity storage of biomass are still disregarded. Recently, the degradation of naphthalene has been shown by Pleurotus eryngii F032, a white‐rot fungus isolated from the tropical rain forest . The fungal growth condition as a critical factor affecting the biotransformation mechanisms of fluoranthene by this fungus has not been investigated.…”