Identification of mutagenic and endocrine disrupting compounds in surface water and wastewater treatment plant effluents using high-resolution effect-directed analysis

Zwart, Nick; Jonker, Willem; Broek, Rob ten; Boer, Jacob de; Somsen, Govert W.; Kool, Jeroen; Hamers, Timo; Houtman, Corine J.; Lamoree, M.H.

doi:10.1016/j.watres.2019.115204

Cited by 66 publications

(48 citation statements)

References 41 publications

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“…Even so, our future research plans include a new sampling campaign to update the levels of ECs in the WWTP effluent of the city of Prague and compare the behavior of these micropollutants over time, and to increase the number of compounds evaluated using Artemia salina and other study model organisms. The new study will possibly include a high-resolution effect-directed analysis (EDA) [6] to optimize analyses of many samples and help identify endocrine disruptors.…”

Section: Effluent Chemical Characterizationmentioning

confidence: 99%

“…Even if appropriate technologies exist, their use is limited because of the incremental cost they represent and the absence of regulations limiting the concentration of the majority of these substances in municipally treated discharge. Discharge and limited wastewater treatment has led to the presence of ECs in water bodies and even drinking water sources around the world [3,6].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Diaz-Sosa

Tapia‐Salazar

Wanner

et al. 2020

Water

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Emerging contaminants (ECs) are not monitored nor regulated consistently, but may have negative effects on human health and ecosystem balance. Although pharmaceuticals and personal care products are among the main ECs found in surface and wastewater, their toxicity and fate are currently not sufficiently studied. In this study, we analyzed for the first time a group of 46 ECs in the secondary effluent of the wastewater treatment plants (WWTP) of Prague. Thirty-seven compounds were identified in the discharge to surface water. Three compounds had no toxicology information on Artemia salina: furosemide, hydrochlorothiazide, and tramadol. We performed acute toxicity (LC50) tests and enzyme assays after 24 and 48 h at room temperature and 28 °C for these three compounds. LC50 ranged from 225.01 mg/L for furosemide, the most toxic, up to above 14,000 mg/L for tramadol. Changes in enzymatic activity for GST, GPx, AChE, and LDH when A. salina were exposed to LC25 for each contaminant were conspicuous and significant in a contaminant-, exposure time-, and temperature-dependent manner. These biochemical markers complement the toxicity profile of these contaminants in aquatic ecosystems and highlight the need for further research on other ECs and their implications, and the regulations required to protect human and ecological health.

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Section: Effluent Chemical Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Diaz-Sosa

Tapia‐Salazar

Wanner

et al. 2020

Water

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“…For these EACs requiring greater sample volumes, it may be feasible to select a bioassay capable of performing in smaller format wells. Although the 96-well format was assessed in the present study, performing the bioassay in a 384-well plate has the potential to decrease EDA volume due to smaller volumes required per well (Zwart et al 2020). However, in 3 out of the 4 studies that performed the bioassays in 384-well plates (Table 1), EDA volumes were among some of the highest volumes assessed (approximately 20-36 L water equivalents; Hashmi et al 2018Hashmi et al , 2020Zwart et al 2018).…”

Section: Water Sampling Volumesmentioning

confidence: 88%

“…The LucTA detector may be more sensitive than the yeast detector for detection of EACs due to the presence of reporter gene signal-enhancing coregulators in the LucTA detector but not the yeast detector (Chu et al 2009). The EACs identified from waters as active components in EDA studies utilizing LucTA assays include estrogen receptor (ER) agonists (E1, E2, E3, EE2, and BPA), an androgen receptor (AR) agonist (ATD), and glucocorticoid receptor (GR) agonists (clobetasol propionate and fluticasone propionate; Furuichi et al 2004;Sonavane et al 2018;Zwart et al 2020). However, more EDA studies utilized a yeast bioassay than a LucTA-type bioassay (Table 1), possibly due to the decreased expense and training required for running yeast bioassays as well as the increased availability of yeast bioassays.…”

Section: Detection Approaches For Edamentioning

confidence: 99%

“…One study identified and quantified carbamazepine in bioactive fractions from river water but did not convert carbamazepine to a CEQ based on estrogenic potency (Lavado et al 2009). Another EDA study identified ATD in androgenic fractions of wastewater influent but did not provide a CEQ value for this EAC (Zwart et al 2020). Lastly, CEQ values may not be provided in a study due to prohibitive costs of the analysis or it may be difficult or impossible to obtain proper standards with which to establish relative potencies.…”

Section: Detection Approaches For Edamentioning

confidence: 99%

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Factors Affecting Sampling Strategies for Design of an Effects‐Directed Analysis for Endocrine‐Active Chemicals

Brennan

Gale

Alvarez

et al. 2020

Enviro Toxic and Chemistry

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Effects‐directed analysis (EDA) is an important tool for identifying unknown bioactive components in a complex mixture. Such an analysis of endocrine‐active chemicals (EACs) from water sources has promising regulatory implications but also unique logistical challenges. We propose a conceptual EDA (framework) based on a critical review of EDA literature and concentrations of common EACs in waste and surface waters. Required water volumes for identification of EACs under this EDA framework were estimated based on bioassay performance (in vitro and in vivo bioassays), limits of quantification by mass spectrometry (MS), and EAC water concentrations. Sample volumes for EDA across the EACs showed high variation in the bioassay detectors, with genistein, bisphenol A, and androstenedione requiring very high sample volumes and ethinylestradiol and 17β‐trenbolone requiring low sample volumes. Sample volume based on the MS detector was far less variable across the EACs. The EDA framework equation was rearranged to calculate detector “thresholds,” and these thresholds were compared with the literature EAC water concentrations to evaluate the feasibility of the EDA framework. In the majority of instances, feasibility of the EDA was limited by the bioassay, not MS detection. Mixed model analysis showed that the volumes required for a successful EDA were affected by the potentially responsible EAC, detection methods, and the water source type, with detection method having the greatest effect on the EDA of estrogens and androgens. The EDA framework, equation, and model we present provide a valuable tool for designing a successful EDA. Environ Toxicol Chem 2020;39:1309–1324. © 2020 SETAC

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Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning

Neale

Escher

Baat

et al. 2023

Enviro Toxic and Chemistry

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Identification of mutagenic and endocrine disrupting compounds in surface water and wastewater treatment plant effluents using high-resolution effect-directed analysis

Cited by 66 publications

References 41 publications

Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Factors Affecting Sampling Strategies for Design of an Effects‐Directed Analysis for Endocrine‐Active Chemicals

Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning

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