1991
DOI: 10.1128/mcb.11.8.4177
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Identification of mRNAs associated with programmed cell death in immature thymocytes.

Abstract: Programmed cell death is an essential cellular process that occurs in epithelial turnover, neural development, and regulation of cell populations of the immune system. Thymocytes undergo programmed cell death in response to several inductive stimuli, including exposure to glucocorticoids or radiation. This program can be blocked by inhibitors of RNA or protein synthesis; this implies that new proteins are required to execute the death programs. To search for possible death-associated mRNAs, we directionally cl… Show more

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Cited by 270 publications
(128 citation statements)
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References 23 publications
(29 reference statements)
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“…This induction is more rapid than that of mdm-2, in agreement with the pro- Table 1 Expression of genes indentified by their diffential expression during apoptosis Gene(s) Induced in Expression in REtsAF Calmodulin, chondroitin sulfate Glucocorticoids induced thymocyte apoptosis [11,20] Not detected proteoglycan core protein RP8 Glutathione S-transferase Ybl Clusterin Ubiquitin Glucocorticoids or radiation induced thymocyte apoptosis [15] Prostate regression [32] and steroid induced lymphocyte apoptosis [21] Prostate regression and various other cell death process [17] Radiation-induced lymphocyte apoptosis [18] and insect muscles degeneration [19] [29], which suggest that, in some cells, p53 can mediate apoptosis by repressing survival genes. Recently, a reevaluation of the role of de novo protein synthesis in thymocyte apoptosis has also suggested that inhibitors of protein synthesis may delay apoptosis rather than prevent it [30], suggesting that some of the components of the apoptotic machinery are already present before apoptosis induction.…”
Section: Discussionsupporting
confidence: 67%
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“…This induction is more rapid than that of mdm-2, in agreement with the pro- Table 1 Expression of genes indentified by their diffential expression during apoptosis Gene(s) Induced in Expression in REtsAF Calmodulin, chondroitin sulfate Glucocorticoids induced thymocyte apoptosis [11,20] Not detected proteoglycan core protein RP8 Glutathione S-transferase Ybl Clusterin Ubiquitin Glucocorticoids or radiation induced thymocyte apoptosis [15] Prostate regression [32] and steroid induced lymphocyte apoptosis [21] Prostate regression and various other cell death process [17] Radiation-induced lymphocyte apoptosis [18] and insect muscles degeneration [19] [29], which suggest that, in some cells, p53 can mediate apoptosis by repressing survival genes. Recently, a reevaluation of the role of de novo protein synthesis in thymocyte apoptosis has also suggested that inhibitors of protein synthesis may delay apoptosis rather than prevent it [30], suggesting that some of the components of the apoptotic machinery are already present before apoptosis induction.…”
Section: Discussionsupporting
confidence: 67%
“…Probes for calmodulin, chondroitin sulfate proteoglycan core protein, clusterin, ubiquitin and GAPDH were obtained, respectively, by labeling of the insert of plasmid p21, p8.5 [11], pSP64-60HE [12], pHUB14.38 [13] and pGAPD-13 [14], respectively, with [32p]dCTP by random primers using the megaprime-labeling systems (Amersham). Probes for RP8, glutathione S-transferase (made from plasmids pRP-8 [15], pGST2 [16]) were labeled with [32p]dCTP by PCR of pBluescript inserts. PCR-radiolabeling reactions (100/21) contained 10 ng of supercoiled plasmid template, 100 pmol of universals primers, 5 nmol each of three nucleotides dATP, dGTP and dTTP, 0.32 nmol dCTP, 2.5/21 of [32p]dCTP (400 Ci/mmol) and 2.5 U ofTaq DNA polymerase (Bioprobe).…”
Section: Rna Analysismentioning
confidence: 99%
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“…27,29 Finally, massive degradation of cellular mRNAs and 28S rRNA take place and further contribute to the translational block under the cell death circumstances. [57][58][59] All these scenarios are described in detail in other chapters of this issue of CDD.…”
Section: Switching the Initiation Of Protein Synthesis From Cap-depenmentioning
confidence: 99%