Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix

Abstract

To identify novel cellular genes that could potentially act as predictive molecular markers for human cervical cancer, we employed RT -PCR differential display, reverse Northern and Northern blot analysis to compare the gene expression profiles between squamous cell carcinoma biopsies and adjacent histo-pathological normal epithelium tissues. Twenty-eight cDNA clones were isolated that were demonstrated to be consistently over-expressed in squamous cell cervical cancer biopsies of FIGO stages 1B to 3B. Most importantly, it was observed that, in addition to their over-expression in cancer lesions, some of these genes are upregulated in the presumably histo-pathological normal adjacent tissues. Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein. When employed for RNA -RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages. In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions. These results allow the distinct possibility of employing the ribosomal protein S12 gene as an early molecular diagnostic identifier for the screening of human cervical cancer and a potential target employed for cancer gene therapy trials.

Ribosomal Proteins and Colorectal Cancer

“…The similar results appeared in carcinoma of breast [9], prostate [3], uterine cervix [10], esophagus [11], liver [12] and also in the glioblastoma and multiforme brain tumors [13].…”

Autoantibodies to Annexin XI-A and Other Autoantigens in the Diagnosis of Breast Cancer

“…The identities of other autoantigens recognized by sera from multiple patients with DCIS and IDC of the breast are listed in Tables 4-6. Some of these phage inserts showed complete identity with partial cDNA sequences encoding for known proteins (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40), whereas other cloned sequences did not show significant homology with Table 1 Potential predictive ability of autoantigens cloned in the T7 phage system The correctly predicted percentages reflect the results of the analysis when one asks the question whether the presence of one or more of the 12 autoantigens that had significant potential for predicting breast cancer can correctly predict breast cancer and whether the absence of all of the phages can predict the absence of cancer. Sensitivity is the percentage of cancer patients correctly predicted.…”

“…In addition, a number of potentially diagnostic autoantigens were recognized by multiple breast cancer sera and had negligible reactivity with control sera, but the differences did not reach significance (Table 5). Among other known and unknown proteins, the ribosomal protein S12 (30,31), the nucleolar protein interacting with the forkhead-associated domain (NIFK) of pKi-67 (34), the p80 subunit of the Ku antigen (28), and cyclin K 33 appear to be promising potential markers. Both cyclin K and the Ku antigen were represented by two clones, but the reactive antigen(s) in one of the phages (UP785) is uncertain because both sequences were cloned from this phage.…”

Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide

“…Recombinant protein expression was assessed after 0, 1, 1.5, 2, 2.5, 3, and 4 h of induction. Proteins were stained with Coomassie blue R-250 and separated by SDS-polyacrylamide gel electrophoresis (PAGE; Cheng et al, 2002;Choveaux et al, 2012;Rodríguez and Hardy, 2015).…”