Ribosomal Proteins and Colorectal Cancer

Lai, Maode; Xu, Jing

doi:10.2174/138920207780076938

Cited by 123 publications

(66 citation statements)

References 47 publications

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“…Indeed, RP gene mutations or altered expression have been found in many human tumors (22,23). In this study, based on Ingenuity Pathway Analysis (Ingenuity Systems, www.ingenuity.com), we chose for validation five of them -RPSA, RPS3, RPS7, RPS14, and RPS29 -all involved in cell cycle regulation, apoptosis, and DNA repair.…”

Section: Discussionmentioning

confidence: 99%

SAGE analysis highlights the putative role of underexpression of ribosomal proteins in GH-secreting pituitary adenomas

Lima

Martins

Paixao

et al. 2012

European Journal of Endocrinology

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Background: Although the molecular pathogenesis of pituitary adenomas has been assessed by several different techniques, it still remains partially unclear. Ribosomal proteins (RPs) have been recently related to human tumorigenesis, but they have not yet been evaluated in pituitary tumorigenesis. Objective: The aim of this study was to introduce serial analysis of gene expression (SAGE), a highthroughput method, in pituitary research in order to compare differential gene expression. Methods: Two SAGE cDNA libraries were constructed, one using a pool of mRNA obtained from five GH-secreting pituitary tumors and another from three normal pituitaries. Genes differentially expressed between the libraries were further validated by real-time PCR in 22 GH-secreting pituitary tumors and in 15 normal pituitaries. Results: Computer-generated genomic analysis tools identified 13 722 and 14 993 exclusive genes in normal and adenoma libraries respectively. Both shared 6497 genes, 2188 were underexpressed and 4309 overexpressed in tumoral library. In adenoma library, 33 genes encoding RPs were underexpressed. Among these, RPSA, RPS3, RPS14, and RPS29 were validated by real-time PCR. Conclusion: We report the first SAGE library from normal pituitary tissue and GH-secreting pituitary tumor, which provide quantitative assessment of cellular transcriptome. We also validated some downregulated genes encoding RPs. Altogether, the present data suggest that the underexpression of the studied RP genes possibly collaborates directly or indirectly with other genes to modify cell cycle arrest, DNA repair, and apoptosis, leading to an environment that might have a putative role in the tumorigenesis, introducing new perspectives for further studies on molecular genesis of somatotrophinomas.

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Section: Discussionmentioning

confidence: 99%

SAGE analysis highlights the putative role of underexpression of ribosomal proteins in GH-secreting pituitary adenomas

Lima

Martins

Paixao

et al. 2012

European Journal of Endocrinology

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“…It is also an established fact that the phenotypic effects of RP genes extend beyond their canonical ribosomal involvement into extraribosomal functions such as DNA replication, transcription, DNA repair, DNA splicing and modification, and apoptosis [1]. In particular, differential expression of ribosomal proteins (RPs) has also been related to cancers [2, 3]. Recently all ribosomal protein genes have been accorded new nomenclature [4], and this is used in this paper we provide, but the old names are provided at their first mention in the text.…”

Section: Introductionmentioning

confidence: 99%

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Sim

Chan

et al. 2016

Disease Markers

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Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes' involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support RPeL27, RPeL41, and RPeL43 as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins.

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“…26 Although different extraribosomal functions have been reported for many r-proteins, but that of RPL18A remains to be elucidated. 27 COII, ND4 and ATP6 are mitochondrial genes involved in oxidative phosphorylation (OXPHOS) biochemical cascade: OXPHOS produces more than 90% of the cellular ATP. The fact that ATP decreases in all cell death mechanisms indicates that energy metabolism might have an important role in tumor cell survival especially under stress conditions including chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization

et al. 2014

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Gastric cancer (GC) is one of the most common and life-threatening types of malignancies. Identification of the differentially expressed genes in GC is one of the best approaches for establishing new diagnostic and therapeutic targets. Furthermore, these investigations could advance our knowledge about molecular biology and the carcinogenesis of this cancer. To screen for the overexpressed genes in gastric adenocarcinoma, we performed suppression subtractive hybridization (SSH) on gastric adenocarcinoma tissue and the corresponding normal gastric tissue, and eight genes were found to be overexpressed in the tumor compared with those of the normal tissue. The genes were ribosomal protein L18A, RNase H2 subunit B, SEC13, eukaryotic translation initiation factor 4A1, tetraspanin 8, cytochrome c oxidase subunit 2, NADH dehydrogenase subunit 4, and mitochondrially encoded ATP synthase 6. The common functions among the identified genes include involvement in protein synthesis, involvement in genomic stability maintenance, metastasis, metabolic improvement, cell signaling pathways, and chemoresistance. Our results provide new insights into the molecular biology of GC and drug discovery: each of the identified genes could be further investigated as targets for prognosis evaluation, diagnosis, treatment, evaluation of the response to new anticancer drugs, and determination of the molecular pathogenesis of GC.

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Ribosomal Proteins and Colorectal Cancer

Cited by 123 publications

References 47 publications

SAGE analysis highlights the putative role of underexpression of ribosomal proteins in GH-secreting pituitary adenomas

SAGE analysis highlights the putative role of underexpression of ribosomal proteins in GH-secreting pituitary adenomas

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization

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