Identification of miR-21-5p/TET1-negative regulation pair in the aggressiveness of glioma cells

Zhuo, Zewei; Yang, Shuzhen; Qi, You-Fu; Yin, Yue

doi:10.5114/fn.2021.108695

Cited by 4 publications

(2 citation statements)

References 21 publications

(24 reference statements)

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“…This work signified that miR-21-5p expressed at high level in brain glioma patients, and modulation of miR-21-5p could impede or advance cell proliferation, invasion, as well as migration. This result was consistent with Zhang et al ( 2021 ), i.e., miR-21-5p was implicated in the brain glioma cell growth and migration and a possible target for treating brain glioma. Glycolysis, as a tumor marker phenomenon, was proposed by Warburg nearly 90 years ago.…”

Section: Discussionsupporting

confidence: 93%

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

Zhang,

Liu

2023

Funct Integr Genomics

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Brain glioma is a common gynecological tumor. MicroRNA (miRNA) plays a very important role in the pathogenesis and development of tumors. It was found that glycolysis played important regulatory roles in tumor growth. The present study aims to investigate the expression pattern of miR-21-5p in brain glioma cells. We examined miR-21-5p and PFKFB2 levels in brain glioma cells via qRT-PCR. Then we performed CCK-8 and Transwell migration assays and determined glucose uptake and lactose production to unveil the properties of miR-21-5p in invasion, cell viability, along with glycolysis in brain glioma cells. Luciferase activity assay was implemented to elucidate if PFKFB2 was a miR-21-5p target gene. Western blotting and qRT-PCR were executed to further validate that miR-21-5p targeted PFKFB2. We repeated these functional assays to observe whether miR-21-5p could impede the function of PFKFB2. qRT-PCR signified that miR-21-5p was elevated in brain glioma tissues in contrast to matching adjacent normal tissues. Functional assays disclosed that elevation of miR-21-5p promoted cell viability, invasion, together with glycolysis. Luciferase assay indicated that PFKFB2 was a miR-21-5p target gene. Moreover, miR-21-inhibit could hinder cell viability, invasion, and glycolysis triggered by overexpression of PFKFB2 in brain glioma cells. miR-21-5p level is elevated in brain glioma and can impede brain glioma cell growth via regulating the glycolysis mediated by PFKFB2, thus is a potential target of treating brain glioma. Graphical abstract

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Section: Discussionsupporting

confidence: 93%

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

Zhang,

Liu

2023

Funct Integr Genomics

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“…Tumors originating from neuroepithelium are collectively referred to as glioma, which is caused by the canceration of glial cells in the cerebrum and spinal cord and is featured by obvious invasiveness, high morbidity, and high death rate [ 1 ]. The pathogenic factors of this disease are relatively complex.…”

Section: Introductionmentioning

confidence: 99%

[Retracted] The Expression Profile of miRNA in Glioma and the Role of miR‐339‐5p in Glioma

Lin

Wang

Shen

et al. 2022

BioMed Research International

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Objective. To reveal the expression profile of miRNA in glioma and the effects of microRNA-339-5p (miR-339-5p) on glioma. Methods. The glioma and normal tissues were randomly selected for miRNA gene chip detection and qRT-PCR verification. The U87 cells were separated into miR-NC, miR-339-5p mimic, and miR-339-5p suppressor group. Clonogenesis test, flow cell technique, Transwell, and cell scratch assay were utilized to verify the roles of miR-339-5p in cell proliferation, cell apoptosis, cell invasion, and cell migration. The epithelial-meso-transformation-associated proteins was verified by Western blot. Results. A total of 49 miRNAs (16 upregulated and 33 downregulated) were differentially expressed in glioma tissues, and miR-339-5p was the most downregulated. The clone number, invasion number, and healing rate of cells in miR-339-5p mimic group were decreased compared with miR-NC group ( P < 0.05 ); the clone quantity, invasion number, and healing rate of cells in miR-339-5p inhibitor group were increased compared with miR-NC group ( P < 0.05 ). The apoptosis rate of human glioma U87 cells in miR-339-5P mimic group was compared with miR-NC group ( P < 0.05 ); the apoptosis rate of human glioma U87 cells in miR-339-5p suppressor group decreased compared with miR-NC group ( P < 0.05 ). Compared with miR-NC group, the protein expression of Twist, Snsnail, N-cadherin, and Vimentin in miR-339-5p mimic group was considerably decreased, whereas E-cadherin was elevated ( P < 0.05 ). Compared with miR-NC group, the protein expression of Twist, Snsnail, N-cadherin, and Vimentin in miR-339-5p suppressor group was considerably increased, whereas E-cadherin was considerably decreased ( P < 0.05 ). Conclusion. Forty-nine glioma-related miRNAs were screened out, and miRNA expression was significantly different between glioma and normal tissues. The downregulated miR-339-5p in glioma can regulate the proliferative, apoptotic, invasive, and migratory abilities of glioma U87 cells and might suppress the occurrence and development of glioma.

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microRNAs expression profile in phyllodes tumors of the breast

Hachana,

Maatouk,

Lassouad

et al. 2024

Heliyon

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Identification of miR-21-5p/TET1-negative regulation pair in the aggressiveness of glioma cells

Cited by 4 publications

References 21 publications

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2

[Retracted] The Expression Profile of miRNA in Glioma and the Role of miR‐339‐5p in Glioma

microRNAs expression profile in phyllodes tumors of the breast

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