[Retracted] The Expression Profile of miRNA in Glioma and the Role of miR‐339‐5p in Glioma

Abstract

Objective. To reveal the expression profile of miRNA in glioma and the effects of microRNA-339-5p (miR-339-5p) on glioma. Methods. The glioma and normal tissues were randomly selected for miRNA gene chip detection and qRT-PCR verification. The U87 cells were separated into miR-NC, miR-339-5p mimic, and miR-339-5p suppressor group. Clonogenesis test, flow cell technique, Transwell, and cell scratch assay were utilized to verify the roles of miR-339-5p in cell proliferation, cell apoptosis, cell invasion, and cell migration. The epithelial-meso-transformation-associated proteins was verified by Western blot. Results. A total of 49 miRNAs (16 upregulated and 33 downregulated) were differentially expressed in glioma tissues, and miR-339-5p was the most downregulated. The clone number, invasion number, and healing rate of cells in miR-339-5p mimic group were decreased compared with miR-NC group ( P < 0.05 ); the clone quantity, invasion number, and healing rate of cells in miR-339-5p inhibitor group were increased compared with miR-NC group ( P < 0.05 ). The apoptosis rate of human glioma U87 cells in miR-339-5P mimic group was compared with miR-NC group ( P < 0.05 ); the apoptosis rate of human glioma U87 cells in miR-339-5p suppressor group decreased compared with miR-NC group ( P < 0.05 ). Compared with miR-NC group, the protein expression of Twist, Snsnail, N-cadherin, and Vimentin in miR-339-5p mimic group was considerably decreased, whereas E-cadherin was elevated ( P < 0.05 ). Compared with miR-NC group, the protein expression of Twist, Snsnail, N-cadherin, and Vimentin in miR-339-5p suppressor group was considerably increased, whereas E-cadherin was considerably decreased ( P < 0.05 ). Conclusion. Forty-nine glioma-related miRNAs were screened out, and miRNA expression was significantly different between glioma and normal tissues. The downregulated miR-339-5p in glioma can regulate the proliferative, apoptotic, invasive, and migratory abilities of glioma U87 cells and might suppress the occurrence and development of glioma.

Prognostic Values of Gene Copy Number Alterations in Prostate Cancer

“…MIR602 is also involved in Hedgehog signalling in chondrocytes and has anti-apoptotic activities in hepatocel-lular carcinoma [69,70]. It is upregulated in gliomas and colon cancers [71,72]. MIR602 overexpression has been associated with a poor prognosis in oesophageal squamous cell carcinoma and in glioblastoma multiforme [68,73] and a favourable outcome in pancreatic ductal adenocarcinoma [74].…”

MicroRNA and mRNA Expression Changes in Glioblastoma Cells Cultivated under Conditions of Neurosphere Formation

“…MiR-339-5p is a tumor suppressor, inhibiting PTP4A1/HMGB1 signal pathway it suppresses vasculogenic mimicry, migration, and invasion of brain glioma U251 cells [ 69 ]. Downregulation of miR-339-5p was recently revealed in glioma [ 70 ], where overexpressed of miR-339-5p has been shown to inhibit the proliferation, invasion and migration of U87 MG human GBM cells and promote programmed cell death.…”

Methods of miRNA delivery and possibilities of their application in neuro-oncology

“…Using mature miRNA mimics is a more convenient and expedited method of intervention [ 3 ]. For example, the transfection of miR-339-5p mimic in vitro demonstrated the potential to suppress the proliferation, invasiveness, migration, and survival of glioblastoma cells [ 25 ].…”