Identification of miR-199a-5p target genes in the skin keratinocyte and their expression in cutaneous squamous cell carcinoma

Kim, Bong-Kyu; Kim, In Jung; Yoon, Sungjoo Kim

doi:10.1016/j.jdermsci.2015.05.005

Cited by 28 publications

(28 citation statements)

References 43 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies revealed that miR‐199a‐5p and the 14‐3‐3beta‐FBI1/Akirin2 complex were involved in the regulation of BCAM expression. In a previous report, miR‐199a‐5p was found to repress BCAM expression by directly targeting its 3′UTR in human keratinocytes (Kim et al , ). BCAM was also suggested as a target gene of the oncogenic 14‐3‐3beta‐FBI1/Akirin2 complex (Akiyama et al , ).…”

Section: Discussionmentioning

confidence: 89%

Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis

et al. 2020

View full text Add to dashboard Cite

Patients with metastatic gastric cancer (GC) have a poor prognosis; however, the molecular mechanism of GC metastasis remains unclear. Here, we employed bioinformatics to systematically screen the metastasis‐associated genes and found that the levels of basal cell adhesion molecule (BCAM) were significantly increased in GC tissues from patients with metastasis, as compared to those without metastasis. The upregulation of BCAM was also significantly associated with a shorter survival time. Depletion of BCAM inhibited GC cell migration and invasion. Knockout (KO) of BCAM by the CRISPR/Cas9 system reduced the invasion and metastasis of GC cells. To explore the mechanism of BCAM upregulation, we identified a previously uncharacterized BCAM sense lncRNA that spanned from exon 6 to intron 6 of BCAM, and named it as BCAM‐associated long noncoding RNA (BAN). Knockdown of BAN inhibited BCAM expression at both mRNA and protein levels. Knockdown of BAN suppressed GC cell migration and invasion, which was effectively rescued by ectopic expression of BCAM. Further clinical data showed that BAN upregulation was associated with GC metastasis and poor prognosis. Importantly, BAN expression was also significantly associated with that of BCAM in GC tissues. Taken together, these results indicate that increased expression of BCAM and its sense lncRNA BAN promote GC cell invasion and metastasis, and are associated with poor prognosis of GC patients.

show abstract

Section: Discussionmentioning

confidence: 89%

Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Alternatively, the up‐regulation of Ccnb 1 and Cdk1 expression by miR‐199a‐5p may play other, yet unidentified roles in mouse keratinocytes. This may be more plausible than a role in cell cycle, because our previous study showed that increased miR‐199a‐5p expression does not induce proliferation in PAM212 cells . The precise role of Ccnb1 / Cdk1 up‐regulation by miR‐199a‐5p in mouse keratinocytes is unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies reported that miR‐199a‐5p is involved in the regulation of biological processes in liver, stomach, testis, colon, and skin keratinocytes . Recently, we performed microarray analysis to identify the target genes of miR‐199a‐5p in mouse skin keratinocytes and showed that Bcam and Fzd6 are new target genes in keratinocytes and human cutaneous squamous cell carcinoma . Among the 393 putative target genes of miR‐199a‐5p, 232 genes were up‐regulated by miR‐199a‐5p overexpression.…”

mentioning

confidence: 98%

Mouse‐specific up‐regulation of Ccnb1 expression by miR‐199a‐5p in keratinocyte

Kim

Lee

et al. 2016

FEBS Open Bio

Self Cite

View full text Add to dashboard Cite

MicroRNA (miRNA) are a class of single‐stranded, small non‐coding RNA that regulate various biological processes, including skin and hair cycle regulation, by modulating the expression of specific genes at the post‐transcriptional level. Recently, several studies reported that miRNA directly or indirectly up‐regulate target genes. Previously, we performed microarray analysis to identify the target genes of miR‐199a‐5p in a mouse skin keratinocyte cell line and detected more than 200 genes whose expression was significantly increased by miR‐199a‐5p overexpression (> 1.5‐fold). In this study, we further investigated these genes and found that cyclin B1 (Ccnb1) expression was positively regulated by miR‐199a‐5p in keratinocyte. Moreover, Ccnb1 expression was inversely correlated with miR‐199a‐5p expression during the mouse hair cycle. Cell cycle analysis showed that the proportion of cells in S phase was slightly increased, while the proportion of cells in G2/M phase decreased by miR‐199‐5p. Using luciferase assay, we found that the 3′ untranslated region of Ccnb1 was a direct target of miR‐199a‐5p. We also found that the regulation of Ccnb1 expression by miR‐199a‐5p is mouse specific. CCNB1 expression was not affected in the human and monkey cell lines. These results provide a new relationship between Ccnb1 and miR‐199a‐5p in both mouse keratinocyte and miRNA biology.

show abstract

“…15,16 The miRNAs play critical roles in various biological processes including regulation of development, cell proliferation, differentiation, apoptosis, cell cycle, stem cell division, and many pathological processes. [17][18][19][20][21][22][23][24] Aberrant miRNA expression has been associated with tumor initiation and progression. Recently, deregulation of miRNAs and their target genes was shown to be closely correlated with proliferation, invasion, and metastasis of multiple tumors including CRC.…”

Section: The Mirnas Affect Gene Expression By Inhibiting Translation mentioning

confidence: 99%

“…The miRNAs affect gene expression by inhibiting translation by binding to the 3′‐UTRs of specific target mRNAs . The miRNAs play critical roles in various biological processes including regulation of development, cell proliferation, differentiation, apoptosis, cell cycle, stem cell division, and many pathological processes . Aberrant miRNA expression has been associated with tumor initiation and progression.…”

Section: Introductionmentioning

confidence: 99%

Decreased expression of VLDLR is inversely correlated with miR‐200c in human colorectal cancer

Kim

Yoo

Lee

et al. 2017

Molecular Carcinogenesis

Self Cite

View full text Add to dashboard Cite

Colorectal cancer (CRC) is one of the most common cancers and has a high rate of morbidity and mortality worldwide. Very-low-density-lipoprotein receptor (VLDLR), a member of the low-density-lipoprotein receptor (LDLR) superfamily, is a multifunctional receptor that regulates cellular signaling by binding numerous ligands. Several studies reported the altered expression of VLDLR and suggested that VLDLR may play a critical role in tumor development by affecting cell proliferation and metastasis. However, the function of VLDLR and regulation of its expression by miRNAs have not been investigated in CRC. In the present study, we investigated the expression of VLDLR in CRC patients and found it to be significantly decreased in tumors in comparison with paired adjacent non-tumor tissues. Moreover, VLDLR over-expression inhibited the proliferation and migration of CRC cells. We also found that VLDLR expression was negatively regulated by miR-200c in CRC cells and that their expression levels were inversely correlated in CRC patients. These data suggest that VLDLR down-regulation mediated by the increased expression of miR-200c may be involved in the development of CRC.

show abstract

Identification of miR-199a-5p target genes in the skin keratinocyte and their expression in cutaneous squamous cell carcinoma

Cited by 28 publications

References 43 publications

Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis

Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis

Mouse‐specific up‐regulation of Ccnb1 expression by miR‐199a‐5p in keratinocyte

Decreased expression of VLDLR is inversely correlated with miR‐200c in human colorectal cancer

Contact Info

Product

Resources

About