Mouse‐specific up‐regulation of Ccnb1 expression by miR‐199a‐5p in keratinocyte

Abstract

MicroRNA (miRNA) are a class of single‐stranded, small non‐coding RNA that regulate various biological processes, including skin and hair cycle regulation, by modulating the expression of specific genes at the post‐transcriptional level. Recently, several studies reported that miRNA directly or indirectly up‐regulate target genes. Previously, we performed microarray analysis to identify the target genes of miR‐199a‐5p in a mouse skin keratinocyte cell line and detected more than 200 genes whose expression was significantly increased by miR‐199a‐5p overexpression (> 1.5‐fold). In this study, we further investigated these genes and found that cyclin B1 (Ccnb1) expression was positively regulated by miR‐199a‐5p in keratinocyte. Moreover, Ccnb1 expression was inversely correlated with miR‐199a‐5p expression during the mouse hair cycle. Cell cycle analysis showed that the proportion of cells in S phase was slightly increased, while the proportion of cells in G2/M phase decreased by miR‐199‐5p. Using luciferase assay, we found that the 3′ untranslated region of Ccnb1 was a direct target of miR‐199a‐5p. We also found that the regulation of Ccnb1 expression by miR‐199a‐5p is mouse specific. CCNB1 expression was not affected in the human and monkey cell lines. These results provide a new relationship between Ccnb1 and miR‐199a‐5p in both mouse keratinocyte and miRNA biology.

CCNB1 and CCNB2 involvement in the pathogenesis of psoriasis: a bioinformatics study

“…[9][10][11] Moreover, high expression of CCNB1 and CCNB2 can promote the proliferation and cell cycle progression of keratinocytes, further exacerbating psoriasis manifestations. 12,13 Bioinformatics analyses by Choudhary and Melero showed that CCNB1 is a core target in the onset mechanism of psoriasis and may contribute to the transformation of mild to severe psoriasis. 14,15 Furthermore, Li et al showed that CCNB2 expression ameliorates the symptoms of psoriasis patients.…”

Analysis of the key prognostic genes and potential traditional Chinese medicine therapeutic targets in glioblastoma based on bioinformatics and network pharmacology methods

“…For our purpose, BIRC5 has been shown to be associated with the occurrence of GBM. G2/mitotic-specific cyclin-B1 (CCNB1) is a key factor in initiating mitosis ( 26 ), which plays a certain regulatory role in G2/M transition ( 27 , 28 ). It performs this by acting as the regulatory subunit of the maturity promoter factor (MPF) and interacting with cyclin-dependent kinase (CDK1).…”

miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G2 phase

“…Kim et al have shown that Ccnb1 is a target of miR-199a-5p in mouse keratinocytes. 33 Others have validated that Ccnb1 is target of miRNA-410 as its overexpression reduces Ccnb1 protein levels and decreases cell proliferation of gonadotroph tumours. 34 Khan et al reported that miR-379 regulates cyclin B1 expression and is decreased in breast cancer.…”

“…17 Recent studies have shown that in addition to the ubiquitination and anaphase-promoting complex mentioned above, there are other factors that participate in cyclin B1 regulation. 31,32 Several studies have shown that miR-199a-5p, 33 miR-410 34 and miR-379 35 mediate the cell cycle by directly regulating cyclin B1 (Ccnb1) expression in several cell types of cells. 27,28 Gene expression is repressed by miRNAs through base-pairing with complementary sequences within mRNA molecules.…”

“…29,30 It is estimated that miRNAs regulate more than 30% of human protein-coding genes, demonstrating the essential role of miRNAs in modulating gene expression. 31,32 Several studies have shown that miR-199a-5p, 33 miR-410 34 and miR-379 35 mediate the cell cycle by directly regulating cyclin B1 (Ccnb1) expression in several cell types of cells.…”