2003
DOI: 10.1002/gcc.10196
|View full text |Cite
|
Sign up to set email alerts
|

Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines

Abstract: At present, there is conflicting evidence whether microsatellite instability (MSI) plays a role in the pathogenesis of breast cancer. Here we describe for the first time an MSI(+) phenotype in two breast cancer cell lines, CAL51 and MT-3, resembling that observed in colorectal cancers. These cell lines are characterized by near-diploid and hyperdiploid karyotypes, respectively. We detected MSI in these cell lines within two non-coding (BAT-25 and BAT-26) and within coding repeat sequences of genes known to be … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
19
0
1

Year Published

2004
2004
2012
2012

Publication Types

Select...
9

Relationship

3
6

Authors

Journals

citations
Cited by 33 publications
(23 citation statements)
references
References 27 publications
3
19
0
1
Order By: Relevance
“…As expected, HMECs and MCF10A cells displayed a simplex profile. Interestingly, CAL51, which harbours DNA mismatch repair (MMR) deficiency and is known to have microsatellite instability [32], displayed minimal changes in the genome, corroborating the hypothesis that cancers/cell lines with MMR deficiency usually have simple karyotypes [33]. No correlation between ER expression and genomic architecture was observed in the cell lines analysed.…”
Section: Genomic Profiling Of Breast Cancer Cell Linessupporting
confidence: 54%
“…As expected, HMECs and MCF10A cells displayed a simplex profile. Interestingly, CAL51, which harbours DNA mismatch repair (MMR) deficiency and is known to have microsatellite instability [32], displayed minimal changes in the genome, corroborating the hypothesis that cancers/cell lines with MMR deficiency usually have simple karyotypes [33]. No correlation between ER expression and genomic architecture was observed in the cell lines analysed.…”
Section: Genomic Profiling Of Breast Cancer Cell Linessupporting
confidence: 54%
“…Surprisingly, we observed several tumors with the "colonic" type MSI, which is very uncommon for cancers of the breast (Adem et al 2003;Anbazhagan et al 1999;Halford et al 2003;Seitz et al 2003;Siah et al 2000;Zhou et al 1998). Here we present an update of our earlier investigation, which contains the following improvements: (1) contrary to the report (Imyanitov et al 2000), the analysis of MSI was the primary goal of this study; therefore, MSI-speciWc markers were used instead of the ones designed for the analysis of losses of heterozygosity; (2) the collection of biBC tumors was signiWcantly enlarged; (3) the control group of unilateral breast carcinomas (BC) was examined as well.…”
Section: Introductionmentioning
confidence: 86%
“…Primer sequences and PCR conditions were described previously [23]. The breast cancer cell line CAL51 was used as positive control [38]. …”
Section: Methodsmentioning
confidence: 99%