Identification of microsatellite DNA markers for population structure analysis in Indian major carp, Cirrhinus mrigala (Hamilton-Buchanan, 1882)

Abstract

Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatellite loci in Indian major carp, Cirrhinus mrigala. A total of 12 loci were successfully amplified with clear scorable patterns and five thereof were polymorphic. Suitability of the identified polymorphic loci in population structure analysis of C. mrigala was assessed. Genetic variation was examined in 76 specimens collected from five different rivers. The mean observed heterozygosity ranged from 0.247 to 0.333. Significant heterogeneity in allele frequencies was detected, indicating that the samples analysed did not belong to homogenous populations. The identified microsatellite markers are promising for the analysis of intraspecific divergence in C. mrigala across its distribution range.

Assessment of Genetic Diversity in Labeo gonius (Hamilton, 1822) through Microsatellite Marker in Nanak Sagar and Dhaura Reservoirs of Uttarakhand, India

“…Same observations (heterozygosity range-0.10-1.00 and 0.500 to 0.870) were reported for other cyprinid fishes like silver carp and bighead carp by Tong et al, (2002); and in three wild and one farm population of L. rohita by Sahoo et al, (2014), respectively. However, the observed heterozygosity range in L. gonius was found to be higher than in Cirrhinus mrigala (0.247 to 0.333) reported from different rivers by Lal et al, (2004). The mean values of observed and expected heterozygosity in L. gonius from Nanak Sagar population using microsatellite marker was found to be comparable with the mean values (0.501 and 0.539) of observed and expected heterozygosity using allozymes marker in L. gonius from Nanak Sagar population (Tewari et al, 2013a).Small differences between observed values of population genetic diversity (Hs=0.4430) and total genetic diversity (Ht=0.5274) indicated moderate genetic differentiation among L. gonius stocks.…”

Microsatellite Markers to Determine Population Genetic Structure in the Golden Anchovy, Coilia dussumieri

“…Generally, the development of new species-specific microsatellite primers is expensive and time consuming, whereas this alternative option is cheap and fast. Primers developed for a species by this method have been successfully tested for cross-species amplification on its related species in several fish species (Zardoya et al 1996;Scribner et al 1996;Galbusera et al 2000;Mohindra et al 2001;Lal et al 2004;Gopalakrishnan et al 2004;Langen et al 2011). In engraulids, microsatellite loci developed in C. ectenes were successfully utilized in stock structure analysis of C. mystus (Ma et al 2011), indicating the usefulness of novel loci for population analysis and kinship studies in anchovies.…”

Identification and characterisation of microsatellite markers in narrow barred Spanish mackerel Scomberomorous commerson (Lacepede, 1800)

“…Fortunately, flanking region similarities between closely related species reduces considerable effort for developing polymorphic microsatellite markers for population genetic analysis (cross-species amplification). This cost-effective alternative for developing polymorphic microsatellite markers is comparatively easier and has been successfully carried out in many fish species for population studies (Lal et al, 2004;Mohitha et al, 2014;Kathirvelpandian et al, 2014). In the present study, development of 11 microsatellite markers in S. commerson through cross-species amplification was carried out from closely related species such as Scomber australasicus, Scomber japonicus, Scomberomorus cavalla and Rastrelliger kanagurta.…”