Identification of microRNAs and Their Target Genes Explores miRNA-Mediated Regulatory Network of Cytoplasmic Male Sterility Occurrence during Anther Development in Radish (Raphanus sativus L.)

Abstract

MicroRNAs (miRNAs) are a type of endogenous non-coding small RNAs that play critical roles in plant growth and developmental processes. Cytoplasmic male sterility (CMS) is typically a maternally inherited trait and widely used in plant heterosis utilization. However, the miRNA-mediated regulatory network of CMS occurrence during anther development remains largely unknown in radish. In this study, a comparative small RNAome sequencing was conducted in floral buds of CMS line ‘WA’ and its maintainer line ‘WB’ by high-throughput sequencing. A total of 162 known miRNAs belonging to 25 conserved and 24 non-conserved miRNA families were isolated and 27 potential novel miRNA families were identified for the first time in floral buds of radish. Of these miRNAs, 28 known and 14 potential novel miRNAs were differentially expressed during anther development. Several target genes for CMS occurrence-related miRNAs encode important transcription factors and functional proteins, which might be involved in multiple biological processes including auxin signaling pathways, signal transduction, miRNA target silencing, floral organ development, and organellar gene expression. Moreover, the expression patterns of several CMS occurrence-related miRNAs and their targets during three stages of anther development were validated by qRT-PCR. In addition, a potential miRNA-mediated regulatory network of CMS occurrence during anther development was firstly proposed in radish. These findings could contribute new insights into complex miRNA-mediated genetic regulatory network of CMS occurrence and advance our understanding of the roles of miRNAs during CMS occurrence and microspore formation in radish and other crops.

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

“…Increasing evidence shows that miRNAs are vital components of the posttranscriptional regulation of gene expression, which is important for almost all aspects of plant biological development, such as tissue differentiation, signal transduction, growth phase switching, and response to stresses (Vance and Vaucheret 2001;Baulcombe 2004;Millar and Gubler 2005;Xie and Qi 2008;Li et al 2011;Soitamo et al 2012;Yang et al 2013a, b, c;Cui et al 2014;Kontra et al 2016;Liu and Chen 2016;Adkar-Purushothama et al 2018;Li and Wang 2018;Zhang et al 2018Zhang et al ,2019Sun Sparks et al 2019). Recently, with the rapid development of high-throughput sequencing technology, several studies have identified miRNAs and their target genes on a genomewide level in crops during anther development (Chambers and Shuai 2009;Wei et al 2013a, b;Yang et al 2013aYang et al , b, c, 2016Jiang et al 2014;Zhang et al 2016Zhang et al ,2018. For example, a comparative miRNAome analysis was performed between CMS and maintainer lines in Raphanus sativus, and 28 known and 14 potential novel miRNAs were detected to be differentially expressed, revealing a putative regulatory network involved in anther development (Zhang et al 2016).…”

Plant Mitochondrial Genome Evolution and Cytoplasmic Male Sterility

“…This link between RNA editing and CMS is not ubiquitous for all plants (Horn et al, 2014). Finally, recent degradome sequencing implicates a possible role for microRNAs in the regulatory network of Brassica juncea and Raphanus sativus CMS Zhang et al, 2016).…”

Mir-24 reduces serum lipid levels and inhibits brain tissue cells apoptosis of rats with cerebral infarction

“…█ INTRODUCTION C erebral infarction is a type of cerebrovascular disease caused by a cerebral blood supply disorder. Severe disturbance of consciousness, sensory and motor dysfunction, neurodegenerative diseases, and even sudden death often occurat the onset of cerebral infarction (17,18,33). It has been reported that cerebral infarction has become a leading cause of disability and death worldwide because of its high morbidity and mortality (9,12).…”