Identification of microphthalmia-associated transcription factor isoforms in dogs

Tsuchida, Shuichi; Takizawa, Takashi; Abe, Katsunori; Okamoto, Masayo; Tagawa, Masahiro

doi:10.1016/j.tvjl.2008.06.004

Cited by 14 publications

(17 citation statements)

References 30 publications

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“…The consecutive first exons of these genes are separately spliced as 1M, 1H, 1A, 1B, 1C, 1D, 1E, 1J and 1mc. Downstream exons 2 to 10 are shared among all isoforms (Tsuchida et al, 2009;Ensembl;AceView). MITF isoform multiplicity with differential expression patterns, as well as functional diversity and redundancy, was explained previously (Fuse et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…S3. (Udono et al, 2000;Shibahara et al, 2001;Hallsson et al, 2007;Tsuchida et al, 2009). Comparison of the MITF genomic DNA and cDNA sequence annotation with other mammalian sequence revealed that the sheep MITF gene consists of 10 exons interrupted by 9 introns (Fig.…”

Section: Cross-species Mitf Amino Acid Sequence Comparisonmentioning

confidence: 99%

“…Each isoform of MITF is differentiated by its unique N-terminus encoded by separate first exons designated as 1M, 1H, 1A, 1B, 1C, 1D, 1E and 1J. All the isoforms of MITF share the entire downstream region containing a transactivation domain and a bHLH-LZ domain encoded by exons 2-9 (Shibahara et al, 2001;Hallsson et al, 2007;Tsuchida et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Alternative splicing of the sheep MITF gene: Novel transcripts detectable in skin

Saravanaperumal

Pediconi

Renieri

et al. 2014

Gene

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Section: Discussionmentioning

confidence: 99%

Section: Cross-species Mitf Amino Acid Sequence Comparisonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alternative splicing of the sheep MITF gene: Novel transcripts detectable in skin

Saravanaperumal

Pediconi

Renieri

et al. 2014

Gene

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“…El gen del factor de transcripción asociado a microftalmia (Microphthalmia-Associated Transcription Factor, MITF) ha sido clonado en varias especies, incluidos seres humanos, ratones, ratas, primates, hámsteres, aves, bovinos, équidos, perros, ranas del género Xenopus y peces, entre otros (8)(9)(10)(11)(12)(13), pero no se ha identificado en cobayos. En todas estas especies, el MITF muestra una gran homología en la estructura, la cual contiene el dominio bHLHZip, que permite el reconocimiento específico de secuencias conocidas como cajas M y E en las regiones promotoras de sus genes diana, así como dos dominios de activación y una secuencia diana de proteína cinasa asociada a microtúbulos (Microtubule-Associated Protein Kinase, MAPK) (14,15).…”

unclassified

“…La expresión del MITF también es regulada por varios promotores alternativos (M, H, A), generando diversas isoformas (18)(19)(20)(21)(22) con funciones biológicas potencialmente distintas (12,13). De esta manera, por ejemplo, el exón 1M es específico de melanocitos, el 1H se expresa en corazón y otro, el 1A, se expresa en más de un tipo celular.…”

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Identificación y expresión diferencial del factor de transcripción asociado a microftalmia en corazón y cardiomiocitos aislados de cobayo, posible papel en hipertrofia y en viabilidad

Gunturiz

Gómez

2014

biomedica

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Genetic and functional evaluation of MITF as a candidate gene for cutaneous melanoma predisposition in pigs

Bourneuf

Estellé

et al. 2011

Mamm Genome

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Cutaneous melanoma arises from transformed melanocytes and is caused mainly by environmental effects such as ultraviolet radiation and to a lesser extent by predisposing genetic variants. Only a few susceptibility genes for cutaneous melanoma have been identified so far in human; therefore, animal models represent a valuable alternative for genetic studies of this disease. In a previous quantitative trait locus (QTL) study, several susceptibility regions were identified in a swine biomedical model, the MeLiM (Melanoblastoma-bearing Libechov minipig) pigs. This article details the fine-mapping of a QTL located on SSC13 (Sus scrofa chromosome 13) through an increase in marker density. New microsatellites were used to confirm the results of the first analysis, and MITF (microphthalmia-associated transcription factor) was selected as a candidate gene for melanoma development. A single-marker association analysis was performed with single-nucleotide polymorphisms (SNPs) spread over the locus, but it did not reveal a significant association with diverse melanoma-related traits. In parallel, MITF alternative transcripts were characterized and their expression was investigated in different porcine tissues. The obtained results showed a complex transcriptional regulation concordant with the one present in other mammals. Notably, the ratio between MITF+ and MITF- isoforms in melanoma samples followed the same pattern as in human tumors, which highlights the adequacy of the MeLiM pig as a model for human melanoma. In conclusion, although MITF does not seem to be the causal gene of the QTL initially observed, we do not exclude a prominent role of its transcription and function in the outbreak and evolution of the tumors observed in pigs.

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Identification of microphthalmia-associated transcription factor isoforms in dogs

Cited by 14 publications

References 30 publications

Alternative splicing of the sheep MITF gene: Novel transcripts detectable in skin

Alternative splicing of the sheep MITF gene: Novel transcripts detectable in skin

Identificación y expresión diferencial del factor de transcripción asociado a microftalmia en corazón y cardiomiocitos aislados de cobayo, posible papel en hipertrofia y en viabilidad

Genetic and functional evaluation of MITF as a candidate gene for cutaneous melanoma predisposition in pigs

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