Identification of Microorganisms by Modern Analytical Techniques

Buszewski, Bogusław; Rogowska, Agnieszka; Pomastowski, Paweł; Złoch, Michał; Railean-Plugaru, Viorica

doi:10.5740/jaoacint.17-0207

Cited by 58 publications

(49 citation statements)

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“…MALDI-TOF MS is a fast and accurate method based on the ionization of intact microorganism cells with short laser pulses and the subsequent acceleration of the particles in a vacuum by way of an electric field. Each microorganism has a specific spectrum profile [46].…”

Section: Microbiomementioning

confidence: 99%

Saliva and Oral Diseases

Martina

Campanati

Diotallevi

et al. 2020

JCM

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Saliva is a fascinating biological fluid which has all the features of a perfect diagnostic tool. In fact, its collection is rapid, simple, and noninvasive. Thanks to several transport mechanisms and its intimate contact with crevicular fluid, saliva contains hundreds of proteins deriving from plasma. Advances in analytical techniques have opened a new era-called "salivaomics"-that investigates the salivary proteome, transcriptome, microRNAs, metabolome, and microbiome. In recent years, researchers have tried to find salivary biomarkers for oral and systemic diseases with various protocols and technologies. The review aspires to provide an overall perspective of salivary biomarkers concerning oral diseases such as lichen planus, oral cancer, blistering diseases, and psoriasis. Saliva has proved to be a promising substrate for the early detection of oral diseases and the evaluation of therapeutic response. However, the wide variation in sampling, processing, and measuring of salivary elements still represents a limit for the application in clinical practice.

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Section: Microbiomementioning

confidence: 99%

Saliva and Oral Diseases

Martina

Campanati

Diotallevi

et al. 2020

JCM

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“…A further acceleration of the time of the detection and identification of microorganisms was possible thanks to the introduction of a new generation of automatic systems for the identification of bacteria based on biochemical properties. The identification of bacterial culture based on the principle of comparing the biochemical reaction profile with the database is most often performed using automated sets such as Analytical Profile Index API ® , BD Phoenix™ Automated Microbiology System or Vitek 2 Compact [100,104]. Commercial automated systems for the identification and determination of bacterial susceptibility are usually based on the same principles as conventional tests, as they use miniaturized versions of these tests.…”

Section: Biochemical Testsmentioning

confidence: 99%

“…Commercial automated systems for the identification and determination of bacterial susceptibility are usually based on the same principles as conventional tests, as they use miniaturized versions of these tests. In addition, automated systems not only determine susceptibility but also indicate the likely mechanism of antibiotic resistance, such as extended-spectrum beta-lactamases (ESBL), methicillin resistance in Staphylococci, glycopeptide resistance or high-degree aminoglycosides resistance in Enterococci [100,107,108].…”

Section: Biochemical Testsmentioning

confidence: 99%

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

2020

Self Cite

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Antibiotics were initially natural substances. However, nowadays, they also include synthetic drugs, which show their activity against bacteria, killing or inhibiting their growth and division. Thanks to these properties, many antibiotics have quickly found practical application in the fight against infectious diseases such as tuberculosis, syphilis, gastrointestinal infections, pneumonia, bronchitis, meningitis and septicemia. Antibiotic resistance is currently a detrimental problem; therefore, in addition to the improvement of antibiotic therapy, attention should also be paid to active metabolites in the body, which may play an important role in exacerbating the existing problem. Taking into account the clinical, cognitive and diagnostic purposes of drug monitoring, it is important to select an appropriate analytical method that meets all the requirements. The detection and identification of the microorganism responsible for the infection is also an essential factor in the implementation of appropriate antibiotic therapy. In recent years, clinical microbiology laboratories have experienced revolutionary changes in the way microorganisms are identified. The MALDI-TOF MS technique may be interesting, especially in some areas where a quick analysis is required, as is the case with clinical microbiology. This method is not targeted, which means that no prior knowledge of the infectious agent is required, since identification is based on a database match.

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“…These techniques are laborious, slow and unspecific in some cases. New methods for the identification of bacteria based on the principles of molecular biology have also been developed and specially polymerase chain reactions (PCR) based methods [ 20 , 21 ]. In the last decade, different methods based on the combination of sensitive techniques such as surface-enhanced Raman spectroscopy (SERS), electrochemical methods or fluorescence methods combined with the use of nanoparticles, especially metal nanoparticles, for rapid and sensitive identification of bacteria have been developed.…”

Section: Nanoparticles For Bacterial Detectionmentioning

confidence: 99%

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

Colino

Millán

Lanao

2018

IJMS

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Advances in nanoparticle-based systems constitute a promising research area with important implications for the treatment of bacterial infections, especially against multidrug resistant strains and bacterial biofilms. Nanosystems may be useful for the diagnosis and treatment of viral and fungal infections. Commercial diagnostic tests based on nanosystems are currently available. Different methodologies based on nanoparticles (NPs) have been developed to detect specific agents or to distinguish between Gram-positive and Gram-negative microorganisms. Also, biosensors based on nanoparticles have been applied in viral detection to improve available analytical techniques. Several point-of-care (POC) assays have been proposed that can offer results faster, easier and at lower cost than conventional techniques and can even be used in remote regions for viral diagnosis. Nanoparticles functionalized with specific molecules may modulate pharmacokinetic targeting recognition and increase anti-infective efficacy. Quorum sensing is a stimuli-response chemical communication process correlated with population density that bacteria use to regulate biofilm formation. Disabling it is an emerging approach for combating its pathogenicity. Natural or synthetic inhibitors may act as antibiofilm agents and be useful for treating multi-drug resistant bacteria. Nanostructured materials that interfere with signal molecules involved in biofilm growth have been developed for the control of infections associated with biofilm-associated infections.

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Identification of Microorganisms by Modern Analytical Techniques

Cited by 58 publications

References 0 publications

Saliva and Oral Diseases

Saliva and Oral Diseases

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

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