Identification of Markers Associated with Wheat Dwarf Virus (WDV) Tolerance/Resistance in Barley (Hordeum vulgare ssp. vulgare) Using Genome-Wide Association Studies

Soleimani, Behnaz; Lehnert, Heike; Trebing, Sarah; Habekuß, Antje; Ordon, Frank; Stahl, Andreas; Will, Torsten

doi:10.3390/v15071568

Cited by 4 publications

(1 citation statement)

References 50 publications

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“…The use of the identified QTL in marker-assisted selection can be achieved by developing PCR-based markers from verified array-based markers. For example, the use of competitive allele-specific PCR markers (KASP) developed from flanking marker sequences offers an efficient approach in hexaploid wheat [319][320][321]. The introduction of WDV tolerance can be facilitated by the use of molecular markers, avoiding artificial inoculation with virus-bearing leafhoppers, which is difficult to integrate into applied breeding programs.…”

Section: Time Eventmentioning

confidence: 99%

The Past, Present, and Future of Wheat Dwarf Virus Management—A Review

Pfrieme,

Will,

Pillen

et al. 2023

Plants

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Wheat dwarf disease (WDD) is an important disease of monocotyledonous species, including economically important cereals. The causative pathogen, wheat dwarf virus (WDV), is persistently transmitted mainly by the leafhopper Psammotettix alienus and can lead to high yield losses. Due to climate change, the periods of vector activity increased, and the vectors have spread to new habitats, leading to an increased importance of WDV in large parts of Europe. In the light of integrated pest management, cultivation practices and the use of resistant/tolerant host plants are currently the only effective methods to control WDV. However, knowledge of the pathosystem and epidemiology of WDD is limited, and the few known sources of genetic tolerance indicate that further research is needed. Considering the economic importance of WDD and its likely increasing relevance in the coming decades, this study provides a comprehensive compilation of knowledge on the most important aspects with information on the causal virus, its vector, symptoms, host range, and control strategies. In addition, the current status of genetic and breeding efforts to control and manage this disease in wheat will be discussed, as this is crucial to effectively manage the disease under changing environmental conditions and minimize impending yield losses.

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Section: Time Eventmentioning

confidence: 99%

The Past, Present, and Future of Wheat Dwarf Virus Management—A Review

Pfrieme,

Will,

Pillen

et al. 2023

Plants

View full text Add to dashboard Cite

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Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat

Pfrieme,

Stahl,

Pillen

et al. 2024

J Plant Dis Prot

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Wheat dwarf virus (WDV) causes high yield losses in wheat and other cereals and is therefore an important pathogen transmitted by the leafhopper Psammotettix alienus. Climate change will increase infections by insect-transmitted viruses due to the increasing spread of vectors. In the context of integrated pest management, the cultivation of WDV-resistant/tolerant varieties is an effective way of controlling WDV. Evaluation of tolerant/resistant genotypes is based on inoculation with viruliferous leafhoppers and subsequent phenotyping in gauze houses under semi-field conditions. For successful screening, it is important to ensure the uniform and reproducible inoculation of plants. Abiotic conditions, particularly temperature, have a critical influence on inoculation success, and thus, variations in infection rates were observed within and between previous replicates in the field. Furthermore, the leafhopper population reared in the greenhouse has to be reestablished after each infection, which delays the screening process. We addressed these issues by developing an improved inoculation assay in which plants are inoculated in small infection hoods in the greenhouse before being planted out in gauze houses. This procedure allows optimal environmental conditions for WDV infection of test plants and allows the plants with WDV infection to develop under natural environmental conditions for symptom scoring. In addition, the viruliferous leafhoppers were recollected from the test plants after infection, allowing a sustainable use of the insects. The method thus enables more reliable phenotyping by increasing infection success and testing a greater number of genotypes in a shorter time.

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Comparative transcriptome analysis of resistant and susceptible watermelon genotypes reveals the role of RNAi, callose, proteinase, and cell wall in squash vein yellowing virus resistance

Kumar,

Chanda,

Adkins

et al. 2024

Front. Plant Sci.

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Watermelon (Citrullus lanatus) is the third largest fruit crop in the world in term of production. However, it is susceptible to several viruses. Watermelon vine decline (WVD), caused by whitefly-transmitted squash vein yellowing virus (SqVYV), is a disease that has caused over $60 million in losses in the US and continues to occur regularly in southeastern states. Understanding the molecular mechanisms underlying resistance to SqVYV is important for effective disease management. A time-course transcriptomic analysis was conducted on resistant (392291-VDR) and susceptible (Crimson Sweet) watermelon genotypes inoculated with SqVYV. Significantly higher levels of SqVYV were observed over time in the susceptible compared to the resistant genotype. The plasmodesmata callose binding protein (PDCB) gene, which is responsible for increased callose deposition in the plasmodesmata, was more highly expressed in the resistant genotype than in the susceptible genotype before and after inoculation, suggesting the inhibition of cell-to-cell movement of SqVYV. The potential role of the RNA interference (RNAi) pathway was observed in the resistant genotype based on differential expression of eukaryotic initiation factor (eIF), translin, DICER, ribosome inactivating proteins, RNA-dependent RNA polymerase (RDR), and Argonaute (AGO) genes after inoculation. The significant differential expression of hormone-related genes, including those involved in the ethylene, jasmonic acid, auxin, cytokinin, gibberellin, and salicylic acid signaling pathways, was observed, emphasizing their regulatory roles in the defense response. Genes regulating pectin metabolism, cellulose synthesis, cell growth and development, xenobiotic metabolism, and lignin biosynthesis were overexpressed in the susceptible genotype, suggesting that alterations in cell wall integrity and growth processes result in disease symptom development. These findings will be helpful for further functional studies and the development of SqVYV-resistant watermelon cultivars.

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Identification of Markers Associated with Wheat Dwarf Virus (WDV) Tolerance/Resistance in Barley (Hordeum vulgare ssp. vulgare) Using Genome-Wide Association Studies

Cited by 4 publications

References 50 publications

The Past, Present, and Future of Wheat Dwarf Virus Management—A Review

The Past, Present, and Future of Wheat Dwarf Virus Management—A Review

Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat

Comparative transcriptome analysis of resistant and susceptible watermelon genotypes reveals the role of RNAi, callose, proteinase, and cell wall in squash vein yellowing virus resistance

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