Identification of Long Noncoding <scp>RNA</scp> Associated with Osteoarthritis in Humans

Xing, Dan; Liang, Jinqian; Li, Yan; Lü, Jun; Jia, Haobo; Xu, Liyan; Ma, Xiaofang

doi:10.1111/os.12147

Cited by 123 publications

(111 citation statements)

References 35 publications

(49 reference statements)

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“…As Steck et al [23] revealed that lncRNA H19 acted as a key regulator in chondrocyte metabolism process in OA. Xing et al [14] reported that lncRNA RP11-445H22.4 was up-regulated in OA compared with normal cartilage tissues. Based on these previous studies, we further explored the effects of lncRNA RP11-445H22.4 on LPS-induced cell injuries in OA.…”

Section: Discussionmentioning

confidence: 99%

“…Recent study demonstrated that lncRNA RP11-445H22.4 was highly expressed in breast cancer cells, and it was related to the occurrence of tumor induced inflammatory response [13]. Furthermore, high expression lncRNA RP11-445H22.4 have been found in OA cartilage cells, and it might be associated with the pathogenesis of OA [14]. But, little is known about the function of lncRNA RP11-445H22.4 in OA and its potential targets for therapy.…”

Section: Introductionmentioning

confidence: 99%

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Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

Sun

Han

et al. 2018

Cell Physiol Biochem

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Background/Aims: Several long non-coding RNAs (lncRNAs) play vital roles in osteoarthritis (OA), whereas the role of lncRNA RP11-445H22.4 in OA remains unclear. The study aimed to investigate the effect of lncRNA RP11-445H22.4 on lipopolysaccharide (LPS)-induced cell viability, apoptosis and inflammatory injury of OA. Methods: The expression of RP11-445H22.4, miR-301a and CXCR4 in human cartilage ATDC5 cells were altered by transfection, and then cells were exposed to 5 µg/ml LPS for 12 h. Then cell viability, apoptosis, apoptosis-related factors and inflammatory cytokines were analyzed by CCK-8, flow cytometry, western blot, RT-qPCR and ELISA, respectively. Dual-luciferase reporter assay was performed to assess the binging sites of RP11-445H22.4 and miR-301a. The signal pathways of NF-κB and MAPK/ ERK were determined by western blot. Results: LPS reduced cell viability, increased apoptosis and stimulated release of IL-1β, IL-6, IL-8 and TNF-α. However, RP11-445H22.4 inhibition significantly rescued LPS-induced injuries by promoting cell viability, suppressing apoptosis and inflammatory cytokines secretions in ATDC5 cells. In addition, miR-301a directly bound to RP11-445H22.4, and suppression of miR-301a inversed the effects of RP11-445H22.4 inhibition. Furthermore, CXCR4 was a direct target of miR-301a, and CXCR4 silencing increased cell viability, decreased apoptosis and inflammatory cytokines secretions in LPS-treated ATDC5 cells. Besides, we found that CXCR4 silencing blocked LPS-activated NF-κB and MAPK/ERK pathways. Conclusions: The study indicated that lncRNA RP11-445H22.4-miR-301a-CXCR4 axis played an important role in cartilage ATDC5 cells and provided a theoretical basis of lncRNA RP11-445H22.4 in OA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

Sun

Han

et al. 2018

Cell Physiol Biochem

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“…Finally, a recent report revealed a new function for H19 in regulating DNMT3B-mediated DNA methylation (137). This is interesting from the standpoint that changes in DNA methylation patterns have been detected in human OA cartilage and that H19 levels have been reported to be higher in OA (134,138). …”

Section: Incrnas In Cartilage Homeostasis and Osteoarthritismentioning

confidence: 90%

“…Other microarray-based studies have been published reporting IncRNA expression changes in OA chondrocytes compared to healthy control cells (138,140,141). One of these studies reported 121 differentially expressed IncRNAs, 73 of which were significantly upregulated including HOTAIR, GAS5, PMS2L2, RP11-445H22.4, H19 , and CTD-2574D22.4 (138).…”

Section: Incrnas In Cartilage Homeostasis and Osteoarthritismentioning

confidence: 99%

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Emerging roles for long noncoding RNAs in skeletal biology and disease

Huynh

Anderson

Guilak

et al. 2016

Connective Tissue Research

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Normal skeletal development requires tight coordination of transcriptional networks, signaling pathways, and biomechanical cues, and many of these pathways are dysregulated in pathological conditions affecting cartilage and bone. Recently, a significant role has been identified for long noncoding RNAs (IncRNAs) in developing and maintaining cellular phenotypes, and improvements in sequencing technologies have led to the identification of thousands of IncRNAs across diverse cell types, including the cells within cartilage and bone. It is clear that IncRNAs play critical roles in regulating gene expression. For example, they can function as epigenetic regulators in the nucleus via chromatin modulation to control gene transcription, or in the cytoplasm, where they can function as scaffolds for protein-binding partners or modulate the activity of other coding and noncoding RNAs. In this review, we discuss the growing list of IncRNAs involved in normal development and/or homeostasis of the skeletal system, the potential mechanisms by which these IncRNAs might function, and recent improvements in the methodologies available to study IncRNA functions in vitro and in vivo. Finally, we address the likely utility of IncRNAs as biomarkers and therapeutic targets for diseases of the skeletal system, including osteoarthritis, osteoporosis, and in cancers of the skeletal system.

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Review: Long Noncoding RNAs in the Regulation of Inflammatory Pathways in Rheumatoid Arthritis and Osteoarthritis

Pearson

Jones

2016

Arthritis & Rheumatology

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Identification of Long Noncoding RNA Associated with Osteoarthritis in Humans

Abstract: The differently expressed lncRNAs may be associated with the pathogenesis of OA. Further functional studies are critical to confirming the function of lncRNAs in OA and to exploring new potential targets for therapy.

Cited by 123 publications

References 35 publications

Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

Emerging roles for long noncoding RNAs in skeletal biology and disease

Review: Long Noncoding RNAs in the Regulation of Inflammatory Pathways in Rheumatoid Arthritis and Osteoarthritis

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