Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion

Abstract

The identification of the expression patterns of long non-coding RNAs (lncRNAs) and mRNAs in the spinal cord under normal and cardiac ischemia/reperfusion (I/R) conditions is essential for understanding the genetic mechanisms underlying the pathogenesis of cardiac I/R injury. The present study used high-throughput RNA sequencing to investigate differential gene and lncRNA expression patterns in the spinal cords of rats during I/R-induced cardiac injury. Male Sprague Dawley rats were assigned to the following groups: i) Control; ii) 2 h (2 h post-reperfusion); and iii) 0.5 h (0.5 h post-reperfusion). Further mRNA/lncRNA microarray analysis revealed that the expression profiles of lncRNA and mRNA in the spinal cords differed markedly between the control and 2 h groups, and in total 7,980 differentially expressed (>2-fold) lncRNAs (234 upregulated, 7,746 downregulated) and 3,428 mRNAs (767 upregulated, 2,661 downregulated) were identified. Reverse transcription-quantitative polymerase chain reaction analysis was performed to determine the expression patterns of several lncRNAs. The results indicated that the expression levels of lncRNA NONRATT025386 were significantly upregulated in the 2 and 0.5 h groups when compared with those in the control group, whereas the expression levels of NONRATT016113, NONRATT018298 and NONRATT018300 were elevated in the 2 h group compared with those in the control group; however, there was no statistically significant difference between the 0.5 h and control groups. Furthermore, the expression of lncRNA NONRATT002188 was significantly downregulated in the 0.5 and 2 h groups when compared with the control group. The present study determined the expression pattern of lncRNAs and mRNAs in rat spinal cords during cardiac I/R. It was suggested that lncRNAs and mRNAs from spinal cords may be novel therapeutic targets for the treatment of I/R-induced cardiac injury.

Investigation of long non‑coding RNA expression profiles in patients with post‑menopausal osteoporosis by RNA sequencing

“…The results suggested that the differentially expressed lncRNAs were accumulated in PMOP-associated signaling pathways, including osteoclast differentiation, as well as TNF and MAPK signaling pathways. Aberrant gene expression profiles have been observed in various diseases, suggesting their potential as diagnostic biomarkers and therapeutic targets (15)(16)(17). The gene expression profiles in osteoporosis remain poorly investigated.…”

Neurochemical alterations of different cerebral regions in rats with myocardial ischemia-reperfusion injury based on proton nuclear magnetic spectroscopy analysis

“…Myocardial IR surgeries were performed as previously described [ 9 , 24 , 25 ]. Briefly, prior to surgery, each rat was deeply anaesthetized and given atropine (0.25-0.3 ml of a 50 mg/ml solution intraperitoneally) to reduce salivation.…”

“…response to internal and external cues [6,7]. Myocardial ischemia-reperfusion injury (MIRI) is known to be associated with comorbid nociceptive disorders and increased mortality risk [8][9][10][11][12]. The mechanisms through which MIRI is linked to these negative outcomes remains unclear, in part due to limited knowledge of the pathophysiology of MIRI itself.…”

Quantitative proteomics reveal the alterations in the spinal�cord after myocardial ischemia‑reperfusion injury in rats

“…The author's previous study showed that transcriptomics and metabolomics enable the examination of spinal biological systems in unprecedented detail (32)(33)(34)(35)(36). More recently, different patterns were revealed in the metabolic and transcriptional levels of the thoracic spinal cord under myocardial ischemia-reperfusion injury (37)(38)(39). Variations in metabolomics and transcriptomics are closely related to proteomics.…”