Identification of lactic acid bacteria in salted Chinese cabbage by SDS-PAGE and PCR-DGGE

Hong, Young Ho; Yang, Hee-Seok; Li, Jingmei; Han, Seon-Kyeong; Chang, Hae-Choon; Kim, Hae‐Yeong

doi:10.1002/jsfa.6257

Cited by 37 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To overcome these problems associated with spontaneous fermentation, inoculation fermentation is currently being investigated. Several studies have attempted to isolate bacterial strains exhibiting excellent fermentation characteristics from NCTFP (Bi, Liu, & Meng, 2000;Hong et al, 2014). Additionally, several species of LABs isolated from naturally fermented products have been used as starter cultures for controlling the fermentation process and improving the quality of the final product during laboratory-scale production in China.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the safety and applications of bacteriocinogenic Enterococcus faecium Y31 as an adjunct culture in North-eastern Chinese traditional fermentation paocai

et al. 2015

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Assessment of the safety and applications of bacteriocinogenic Enterococcus faecium Y31 as an adjunct culture in North-eastern Chinese traditional fermentation paocai

et al. 2015

View full text Add to dashboard Cite

“…16S rRNA sequencing is commonly used for bacterial identification, including the identification of Lactobacillus species [13][14][15]. Metagenome sequencing and DGGE based on 16S rRNA gene sequences are useful analytical methods for investigating complex microbial communities without previous isolation of individual bacteria [16][17][18]. However, 16S rRNA gene sequences in many Lactobacillus species are too similar to be readily distinguished.…”

Section: Introductionmentioning

confidence: 99%

Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene sequences. In this study, we developed a rapid and accurate method based on comparative genomic analysis for the identification of 37 Lactobacillus species that are commonly used in probiotics and fermented foods. Results: To select species-specific sequences or genes, a total of 180 Lactobacillus genome sequences were compared using Python scripts. In 14 out of 37 species, species-specific sequences could not be found due to the similarity of the 16S-23S rRNA gene. Selected unique genes were obtained using comparative genomic analysis and all genes were confirmed to be specific for 52,478,804 genomes via in silico analysis; they were found not to be strain-specific, but to exist in all strains of the same species. Species-specific primer pairs were designed from the selected 16S-23S rRNA gene sequences or unique genes of species. The specificity of the species-specific primer pairs was confirmed using reference strains, and the accuracy and efficiency of the polymerase chain reaction (PCR) with the standard curve were confirmed. The PCR method developed in this study is able to accurately differentiate species that were not distinguishable using the 16S rRNA gene alone. This PCR assays were designed to detect and identify 37 Lactobacillus species. The developed method was then applied in the monitoring of 19 probiotics and 12 dairy products. The applied tests confirmed that the species detected in 17 products matched those indicated on their labels, whereas the remaining products contained species other than those appearing on the label. Conclusions: The method developed in this study is able to rapidly and accurately distinguish different species of Lactobacillus, and can be used to monitor specific Lactobacillus species in foods such as probiotics and dairy products.

show abstract

“…SDS-PAGE method has a discriminatory resolution to the level of species and subspecies (Vandamme et al 1996). Finger printing of total protein using SDS-PAGE has been applied to differentiate strains of Xanthomonas (Vandamme et al 1996;Vauterin et al 1993), members of the genus Bacillus (Berber 2004;Berber & Yenidunya 2005), members of Staphylococci (Santos et al 2012), and the group of Lactic Acid Bacteria (Ghazi et al 2009;Hong et al 2014).…”

Section: Identification Of the Amylolytic Bacteria Producing Of Phb Bmentioning

confidence: 99%

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

Yanti¹,

Sembiring²,

Margino³

et al. 2019

JSM

View full text Add to dashboard Cite

The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data set based on phenotypic characteristics, namely morphological, physiological, biochemical and chemical character, namely whole cells protein profiles using SDS-PAGE method, together with phylogenetic studies based on 16S rRNA sequences was used to identified by polyphasic approach. Phenotypic characteristics of 3 local bacterial isolates and 4 reference strains to members of genus Bacillus was analyzed by numerical analysis using MVSP 3,1 program to determine the value of similarity. Based on the preliminary characterization of the profile matching method showed that the three isolates of bacteria producing PHB namely PSA10, PPK5 and PPK6 are members of the genus Bacillus. The results of numerical analysis based on phenotypic characteristic and chemical character of the three bacterial isolates producing PHB with reference strains showed that the PSA10 isolate bacterial identical with Bacillus megaterium, PPK5 isolate identical with Bacillus subtilis and PPK6 isolate identical with Bacillus cereus, and these results also support by the molecular phylogenetic analysis. Therefore, the polyphasic taxonomy is an effective approach to uncover the identity of the novel bacterial isolates. ABSTRAKMatlamat akhir kajian ini adalah untuk membuat inventori berasaskan sistematik moden daripada pencilan bakteria amilolisis yang menghasilkan bioplastik Poli-β-hidroksibutirat (PHB) daripada substrat kanji sagu. Identiti tiga pencilan bakteria tempatan telah diperiksa dalam kajian ini, menggunakan pendekatan polifasa. Satu set data berdasarkan ciriciri fenotip, iaitu sifat morfologi, fisiologi, biokimia dan kimia, iaitu profil protein sel keseluruhan menggunakan kaedah SDS-PAGE, bersama-sama dengan kajian filogenetik berdasarkan urutan 16S rRNA digunakan untuk mengenal pasti dengan pendekatan polifasa. Ciri-ciri fenotip 3 pencilan bakteria tempatan dan 4 rujukan kepada ahli genus Bacillus dianalisis dengan analisis berangka menggunakan MVSP 3,1 program untuk menentukan nilai keserupaan. Berdasarkan pencirian permulaan kaedah pencocokan profil menunjukkan bahawa tiga pencilan bakteria yang menghasilkan PHB iaitu PSA10, PPK5 dan PPK6 adalah ahli genus Bacillus. Hasil analisis berangka berdasarkan pencirian fenotip dan sifat kimia ketiga pencilan bakteria yang menghasilkan PHB dengan strain rujukan menunjukkan bahawa PSA10 seiras dengan Bacillus megaterium, PPK5 seiras dengan Bacillus subtilis dan PPK6 seiras dengan Bacillus cereus, dan keputusan ini juga disokong oleh analisis filogenetik molekul. Oleh itu, taksonomi polifasa adalah pendekatan yang berkesan untuk mendedahkan identiti novel pencilan bakteria.

show abstract

Identification of lactic acid bacteria in salted Chinese cabbage by SDS-PAGE and PCR-DGGE

Cited by 37 publications

References 17 publications

Assessment of the safety and applications of bacteriocinogenic Enterococcus faecium Y31 as an adjunct culture in North-eastern Chinese traditional fermentation paocai

Assessment of the safety and applications of bacteriocinogenic Enterococcus faecium Y31 as an adjunct culture in North-eastern Chinese traditional fermentation paocai

Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

Contact Info

Product

Resources

About