Identification of key residues for efficient glucose transport by the hexose transporter CgHxt4 in high sugar fermentation yeast Candida glycerinogenes

Qiao, Yanming; Li, Cuili; Lu, Xiaoyun; Zong, Hong; Zhuge, Bin

doi:10.1007/s00253-021-11567-6

Cited by 5 publications

(3 citation statements)

References 56 publications

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“…23 Hyperosmotic tolerance and high temperature resistance are the most important physiological characteristics of C. glycerinogenes. 2,24 High osmotic pressure or high temperature are ideal conditions, which can reduce the risk of bacterial infections. The osmoresponsive promoters P STL1 , P Hxt4 , P YWP1 , P ZWF , and high temperature inducible promoters P AAC , P CWP1 , and P Hsp12 from C. glycerinogenes were chosen to construct the toxin assay vectors (Figure S8).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Candida glycerinogenes (WL2002−5) is an industrial glycerolproducing strain 1 with the advantages of fast glucose transport and short fermentation cycle. 2 In addition, it can withstand environmental stress such as ultrahigh osmotic pressure, 3 high temperature, and a low pH, 4 making it an excellent candidate to be the industrial technology host. Based on this, C. glycerinogenes can produce a variety of high valued compounds, such as 2-phenylethanol, 5 caffeic acid, 6 pinene, 7 etc.…”

Section: ■ Introductionmentioning

confidence: 99%

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Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette

Lv,

Lu,

Zhuge

et al. 2024

ACS Synth. Biol.

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Candida glycerinogenes is an industrial yeast with excellent multistress resistance. However, due to the diploid genome and the lack of meiosis and screening markers, its molecular genetic operation is limited. Here, a gene editing system using the toxin−antitoxin pair relBE from the type II toxin−antitoxin system in Escherichia coli as a screening marker was constructed. The RelBE complex can specifically and effectively regulate cell growth and arrest through a conditionally controlled toxin RelE switch, thereby achieving the selection of positive recombinants. The constructed editing system achieved precise gene deletion, replacement, insertion, and gene episomal expression in C. glycerinogenes. Compared with the traditional amino acid deficiency complementation editing system, this editing system produced higher biomass and the gene deletion efficiency was increased by 3.5 times. Using this system, the production of 2-phenylethanol by C. glycerinogenes was increased by 11.5−13.5% through metabolic engineering and tolerance engineering strategies. These results suggest that the stable gene editing system based on toxin−antitoxin pairs can be used for gene editing of C. glycerinogenes to modify metabolic pathways and promote industrial applications. Therefore, the constructed gene editing system is expected to provide a promising strategy for polyploid industrial microorganisms lacking gene manipulation methods.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette

Lv,

Lu,

Zhuge

et al. 2024

ACS Synth. Biol.

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“…As a high glycerol-producing industrial strain, C. glycerinogenes exhibits many advantages, such as a fast sugar consumption rate, high conversions, and a short fermentation cycle. , Moreover, it can tolerate environmental stresses such as ultra-high osmotic pressure, oxidation, high heat, and a low pH, making it an excellent candidate for a chassis cell production platform.…”

Section: Introductionmentioning

confidence: 99%

Candida glycerinogenes-Promoted α-Pinene and Squalene Co-production Strategy Based on α-Pinene Stress

Zong

et al. 2023

J. Agric. Food Chem.

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α-Pinene is a naturally occurring monoterpene, which is widely used in fragrances, cosmetics, and foods. Due to the high cellular toxicity of α-pinene, this work considered the application of Candida glycerinogenes, an effective industrial strain with high resistance, in α-pinene synthesis. It was found that α-pinene-induced stress resulted in an intracellular accumulation of reactive oxygen species with an increased formation of squalene as a cytoprotective compound. As squalene is a downstream product in the mevalonate (MVA) pathway for α-pinene synthesis, a strategy based on the promotion of α-pinene and squalene co-production under α-pinene stress is proposed. By introducing the α-pinene synthesis pathway and enhancing the MVA pathway, the production of both α-pinene and squalene is increased. We have demonstrated that intracellular synthesis of α-pinene is effective in promoting squalene synthesis. The generation of intercellular reactive oxygen that accompanies α-pinene synthesis promotes squalene synthesis with a resultant cellular protection and upregulation of MVA pathway genes that facilitate α-pinene production. In addition, we have overexpressed phosphatase and introduced NPP as a substrate to synthesize α-pinene, where co-dependent fermentation yielded 208 mg/L squalene and 12.8 mg/L α-pinene. This work establishes a viable strategy to promote terpene-co-dependent fermentation based on stress.

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Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway

Zong

et al. 2022

J Microbiol.

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Identification of key residues for efficient glucose transport by the hexose transporter CgHxt4 in high sugar fermentation yeast Candida glycerinogenes

Cited by 5 publications

References 56 publications

Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette

Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette

Candida glycerinogenes-Promoted α-Pinene and Squalene Co-production Strategy Based on α-Pinene Stress

Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway

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