Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette
Wen Lv,
Xinyao Lu,
Bin Zhuge
et al.
Abstract:Candida glycerinogenes is an industrial yeast with excellent multistress resistance. However, due to the diploid genome and the lack of meiosis and screening markers, its molecular genetic operation is limited. Here, a gene editing system using the toxin−antitoxin pair relBE from the type II toxin−antitoxin system in Escherichia coli as a screening marker was constructed. The RelBE complex can specifically and effectively regulate cell growth and arrest through a conditionally controlled toxin RelE switch, the… Show more
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